Spock1 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Spock1provided by MGI
Official Full Name: sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1provided by MGI
Primary source: MGI:MGI:105371
See related: Ensembl:ENSMUSG00000056222 AllianceGenome:MGI:105371
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Spock; Ticn1; testican
Summary: Predicted to enable calcium ion binding activity; cysteine-type endopeptidase inhibitor activity; and metalloendopeptidase inhibitor activity. Involved in central nervous system neuron differentiation and neuron migration. Located in neuromuscular junction; node of Ranvier; and sarcoplasm. Is active in postsynaptic density. Is expressed in several structures, including jaw; nervous system; sensory organ; skeletal musculature; and thymus primordium. Human ortholog(s) of this gene implicated in hepatocellular carcinoma. Orthologous to human SPOCK1 (SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in cerebellum adult (RPKM 21.7), cortex adult (RPKM 19.5) and 7 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 B1; 13 30.5 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (57569008..58056194, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (57421195..57908332, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The secreted neuronal signal Spock1 promotes blood-brain barrier development.
Title: The secreted neuronal signal Spock1 promotes blood-brain barrier development.
Testican-1 blood level is related to the severity of sepsis and Testican-1 could be used as a biomarker to determine the severity of sepsis.
Title: Testican-1, as a novel diagnosis of sepsis.
results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury
Title: Altered expression pattern of testican-1 mRNA after brain injury.
Testican-1 is not critical for mouse development [review]
Title: Testican-1 is dispensable for mouse development.
limits the accumulation of adipose tissue in mice in part through its demonstrated effects on the regulation of cell shape and production of extracellular matrix
Title: SPARC-null mice exhibit increased adiposity without significant differences in overall body weight.
SPARC is counter-adhesive for murine lens epithelial cells. We also identify SPARC as a modulator of LN-1 secretion and deposition, an activity important in epithelial cell-ECM interactions in the ocular lens.
Title: Absence of SPARC in lens epithelial cells results in altered adhesion and extracellular matrix production in vitro.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1)
Targeted (1) 1 citation

General gene information

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Markers

1446 (e-PCR)
- UniSTS:45161

MHAa20b7.seq (e-PCR)
- UniSTS:125069

D13Mit52 (e-PCR), detects polymorphism
- UniSTS:127152

D13Mit65 (e-PCR), detects polymorphism
- UniSTS:127163

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables collagen binding	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables cysteine-type endopeptidase inhibitor activity	ISS Inferred from Sequence or Structural Similarity more info
enables extracellular matrix binding	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables metalloendopeptidase inhibitor activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in central nervous system neuron differentiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in negative regulation of cell-substrate adhesion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell-substrate adhesion	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of endopeptidase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of endopeptidase activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron projection development	ISS Inferred from Sequence or Structural Similarity more info
involved_in neurogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in neuron migration	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in extracellular space	ISS Inferred from Sequence or Structural Similarity more info
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in node of Ranvier	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in sarcoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: testican-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001166463.1 → NP_001159935.1 testican-1 isoform 3 precursor

See identical proteins and their annotated locations for NP_001159935.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an in-frame small exon in the 5' coding region, as compared to variant 1. The resulting isoform (3) is 3 aa shorter than isoform 1.

Source sequence(s)

AC154748, AK051191, BY232939

Consensus CDS

CCDS49279.1

UniProtKB/Swiss-Prot

E9PYW3, Q62288

UniProtKB/TrEMBL

Q8BKQ3

Related

ENSMUSP00000140755.2, ENSMUST00000186271.7

Conserved Domains (4) summary

smart00280
Location:137 → 178

KAZAL; Kazal type serine protease inhibitors

cd00191
Location:312 → 373

TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases

cd00252
Location:197 → 315

SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...

pfam10591
Location:196 → 302

SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region
NM_001166464.1 → NP_001159936.1 testican-1 isoform 4 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (4) has an alternate 3' sequence, as compared to variant 1. The resulting isoform (4) is shorter and has a distinct C-terminus, as compared to isoform 1.

Source sequence(s)

AK036189, AK039058

Consensus CDS

CCDS79194.1

UniProtKB/TrEMBL

A0A087WPP6, Q8BM19

Related

ENSMUSP00000139863.2, ENSMUST00000189373.8

Conserved Domains (3) summary

smart00280
Location:140 → 181

KAZAL; Kazal type serine protease inhibitors

cd00252
Location:200 → 318

SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...

pfam10591
Location:199 → 305

SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region
NM_001166465.1 → NP_001159937.1 testican-1 isoform 5 precursor

See identical proteins and their annotated locations for NP_001159937.1

Status: VALIDATED

Description

Transcript Variant: This variant (5) lacks an in-frame small exon in the 5' coding region, and has an alternate 3' sequence, as compared to variant 1. The resulting isoform (5) is shorter and has a distinct C-terminus, as compared to isoform 1.

Source sequence(s)

AK036189, AK039058, BY232939

Consensus CDS

CCDS79193.1

UniProtKB/TrEMBL

Q8BM07, Q8BM19

Related

ENSMUSP00000141130.2, ENSMUST00000187852.7

Conserved Domains (3) summary

smart00280
Location:137 → 178

KAZAL; Kazal type serine protease inhibitors

cd00252
Location:197 → 315

SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...

pfam10591
Location:196 → 302

SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region
NM_001166466.1 → NP_001159938.1 testican-1 isoform 2 precursor

See identical proteins and their annotated locations for NP_001159938.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate splice site, as compared to variant 1. The reading frame is not changed and the resulting isoform (2) lacks an internal aa, as compared to isoform 1.

Source sequence(s)

AC154748, BB628964, BC158020

UniProtKB/Swiss-Prot

E9PYW3, Q62288

UniProtKB/TrEMBL

B2RXY1

Conserved Domains (4) summary

smart00280
Location:140 → 181

KAZAL; Kazal type serine protease inhibitors

cd00191
Location:314 → 375

TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases

cd00252
Location:200 → 317

SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...

pfam10591
Location:199 → 304

SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region
NM_009262.3 → NP_033288.2 testican-1 isoform 1 precursor

See identical proteins and their annotated locations for NP_033288.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC154748, BC075625, BY241184

Consensus CDS

CCDS79195.1

UniProtKB/Swiss-Prot

E9PYW3, Q62288

UniProtKB/TrEMBL

Q6DIC9

Related

ENSMUSP00000140409.2, ENSMUST00000185502.7

Conserved Domains (4) summary

smart00280
Location:140 → 181

KAZAL; Kazal type serine protease inhibitors

cd00191
Location:315 → 376

TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases

cd00252
Location:200 → 318

SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...

pfam10591
Location:199 → 305

SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

57569008..58056194 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036157954.1 → XP_036013847.1 testican-1 isoform X1

UniProtKB/Swiss-Prot

E9PYW3, Q62288

Conserved Domains (4) summary

cd00191
Location:311 → 372

TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases

cd00252
Location:244 → 275

EFh_SPARC_EC; EF-hand motif [structural motif]

cl25349
Location:198 → 307

EFh_SPARC_EC; EF-hand, extracellular calcium-binding (EC) motif, found in secreted protein acidic and rich in cysteine (SPARC)-like proteins

pfam07648
Location:136 → 173

Kazal_2; Kazal-type serine protease inhibitor domain