CHLI1 P-loop containing nucleoside triphosphate hydrolases superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: CHLI1
Official Full Name: P-loop containing nucleoside triphosphate hydrolases superfamily protein
Primary source: TAIR:AT4G18480
Locus tag: AT4G18480
See related: Araport:AT4G18480
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: CH-42; CH42; CHL11; CHLI-1; CHLORINA 42; F28J12.140; F28J12_140; LOST1; low temperature with open-stomata 1; PROTOPORPHYRIN-IX MG-CHELATASE
Summary: Encodes the CHLI subunit of magnesium chelatase which is required for chlorophyll biosynthesis. All four cysteine residues of the protein form two disulfide bonds (Cys102-Cys193 and Cys354-Cys396) under oxidized conditions but are fully reduced by reduction. It was suggested that the redox state of CHLI is regulated in vivo by the change of the redox environment in the chloroplasts probably via the Trx system.
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Genomic context

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See CHLI1 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 3

Sequence:: Chromosome: 4; NC_003075.7 (10201672..10203469, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The chlorophyll synthesis enzymes, Mg-chelatase complex and Mg-protoporphyrin IX methyltransferase, specifically affect abscisic acid (ABA) signaling in the control of stomatal aperture and have no effect on ABA-induced gene expression.
Title: Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana.
Paths of evolutionary dynamics for CHLI 1 and CHLI 2 genes determining different forms of subunit I of Mg-chelatase complex of Arabidopsis thaliana are proposed.
Title: [Evolution aspects of intraspecific polymorphysm of Arabidopsis thaliana genes coding subunit I of Mg-chelatase complex].
GUN4 stimulating chlorophyll biosynthesis not only by activating Mg-chelatase but also by promoting interactions between ChlH and chloroplast membranes
Title: Porphyrins promote the association of GENOMES UNCOUPLED 4 and a Mg-chelatase subunit with chloroplast membranes.
CHLI2 could support some chlorophyll biosynthesis in the complete absence of CHLI1.
Title: Arabidopsis CHLI2 can substitute for CHLI1.
CHLI2 also has ATPase activity but with a lower Vmax and higher Km ATP than CHLI1
Title: Functional analysis of Arabidopsis thaliana isoforms of the Mg-chelatase CHLI subunit.
Comparison of the amino acid sequence of CHLI 1 and CHLI 2 encoded in the genome of acifluorfen herbicide resistant mutant aci5 and wild type revealed in particular alterations of the C-terminal end.
Title: The analysis of the ChlI 1 and ChlI 2 genes using acifluorfen-resistant mutant of Arabidopsis thaliana.
Gun4 is involved in the stimulation of Mg-chelatase activity.
Title: Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_117962.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables magnesium chelatase activity	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in chlorophyll biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chlorophyll biosynthetic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast stroma	HDA more info	PubMed
located_in chloroplast stroma	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
part_of magnesium chelatase complex	TAS Traceable Author Statement more info	PubMed
located_in plant-type cell wall	HDA more info	PubMed

General protein information

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Preferred Names: P-loop containing nucleoside triphosphate hydrolases superfamily protein

NP_193583.1

CHLI1; FUNCTIONS IN: magnesium chelatase activity, ATPase activity; INVOLVED IN: chlorophyll biosynthetic process; LOCATED IN: magnesium chelatase complex, cell wall, chloroplast, chloroplast stroma; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), Magnesium chelatase, ChlI subunit (InterPro:IPR000523), Magnesium chelatase, ATPase subunit I (InterPro:IPR011775); BEST Arabidopsis thaliana protein match is: magnesium chelatase i2 (TAIR:AT5G45930.1); Has 6584 Blast hits to 6581 proteins in 1594 species: Archae - 309; Bacteria - 5009; Metazoa - 2; Fungi - 0; Plants - 206; Viruses - 0; Other Eukaryotes - 1058 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

10201672..10203469 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_117962.3 → NP_193583.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_193583.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P16127, Q0WUK7

UniProtKB/TrEMBL

A0A178UXM0, A0A5S9XUB5, A0A7G2F450

Conserved Domains (1) summary

CHL00081
Location:77 → 424

chlI; Mg-protoporyphyrin IX chelatase