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    Med8 mediator complex subunit 8 [ Mus musculus (house mouse) ]

    Gene ID: 80509, updated on 13-Jan-2025

    Summary

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    Official Symbol
    Med8provided by MGI
    Official Full Name
    mediator complex subunit 8provided by MGI
    Primary source
    MGI:MGI:1915269
    See related
    Ensembl:ENSMUSG00000006392 AllianceGenome:MGI:1915269
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    ARC32; 2210021A15Rik
    Summary
    Predicted to enable RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and transcription coregulator activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Predicted to be part of core mediator complex and mediator complex. Is expressed in brain; cerebral cortex; telencephalon ventricular layer; tibialis anterior; and tongue. Orthologous to human MED8 (mediator complex subunit 8). [provided by Alliance of Genome Resources, Jan 2025]
    Expression
    Ubiquitous expression in CNS E11.5 (RPKM 17.5), liver E14 (RPKM 15.3) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Med8 in Genome Data Viewer
    Location:
    4 D2.1; 4 54.61 cM
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (118266495..118273021)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (118409282..118415824)

    Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene protein tyrosine phosphatase receptor type F Neighboring gene predicted gene, 52705 Neighboring gene SZT2 subunit of KICSTOR complex Neighboring gene hydroxypyruvate isomerase (putative) Neighboring gene STARR-positive B cell enhancer ABC_E1281 Neighboring gene STARR-positive B cell enhancer ABC_E6202 Neighboring gene ELOVL fatty acid elongase 1 Neighboring gene cell division cycle 20 Neighboring gene myeloproliferative leukemia virus oncogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Szt2, a novel gene for seizure threshold in mice. Frankel WN, et al. Genes Brain Behav, 2009 Jul. PMID 19624305, Free PMC Article
    2. Mediator complex proximal Tail subunit MED30 is critical for Mediator core stability and cardiomyocyte transcriptional network. Tan C, et al. PLoS Genet, 2021 Sep. PMID 34506481, Free PMC Article
    3. Temporal regulation of the Mediator complex during muscle proliferation, differentiation, regeneration, aging, and disease. Kolonay DW, et al. Front Cell Dev Biol, 2024. PMID 38690566, Free PMC Article
    4. Novel critical role of a human Mediator complex for basal RNA polymerase II transcription. Mittler G, et al. EMBO Rep, 2001 Sep. PMID 11559591, Free PMC Article
    5. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. Tomomori-Sato C, et al. J Biol Chem, 2004 Feb 13. PMID 14638676

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Clone Names

    • MNCb-2386

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coregulator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 8
    Names
    activator-recruited cofactor 32 kDa component
    mediator of RNA polymerase II transcription, subunit 8 homolog

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001290688.1 → NP_001277617.1  mediator of RNA polymerase II transcription subunit 8 isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site in te 5' coding region, resulting in initiation of translation at a downstream start site, compared to variant 1. It encodes isoform 3, which is shorter at the N-terminus, compared to isoform 1.
      Source sequence(s)
      AK008759, AK011080, AK148025, BC051683, BY429746
      Consensus CDS
      CCDS71459.1
      UniProtKB/TrEMBL
      Q9CY05
      Related
      ENSMUSP00000081346.5, ENSMUST00000084319.11
      Conserved Domains (1) summary
      pfam10232
      Location:1 → 176
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    2. NM_020000.3 → NP_064384.2  mediator of RNA polymerase II transcription subunit 8 isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AK008759, AK011080, AK148025, BY429746
      Consensus CDS
      CCDS38854.1
      UniProtKB/Swiss-Prot
      Q3UGB4, Q99LM4, Q9D7W5, Q9JJ84
      Related
      ENSMUSP00000019229.9, ENSMUST00000019229.15
      Conserved Domains (2) summary
      pfam09606
      Location:196 → 253
      Med15; ARC105 or Med15 subunit of Mediator complex non-fungal
      pfam10232
      Location:1 → 265
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    3. NM_173719.3 → NP_776067.1  mediator of RNA polymerase II transcription subunit 8 isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) includes an alternate in-frame exon in the 5' coding region and uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter, compared to isoform 1.
      Source sequence(s)
      AK005422, AK011080, AK148025, BY429746
      Consensus CDS
      CCDS18548.1
      UniProtKB/TrEMBL
      Q9DAY7
      Related
      ENSMUSP00000101992.4, ENSMUST00000106384.10
      Conserved Domains (1) summary
      pfam10232
      Location:1 → 231
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000070.7 Reference GRCm39 C57BL/6J

      Range
      118266495..118273021
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006503491.5 → XP_006503554.1  mediator of RNA polymerase II transcription subunit 8 isoform X1

      Conserved Domains (2) summary
      pfam09606
      Location:212 → 269
      Med15; ARC105 or Med15 subunit of Mediator complex non-fungal
      pfam10232
      Location:1 → 281
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    2. XM_006503492.4 → XP_006503555.1  mediator of RNA polymerase II transcription subunit 8 isoform X2

      UniProtKB/TrEMBL
      Q9CY05
      Conserved Domains (1) summary
      pfam10232
      Location:1 → 176
      Med8; Mediator of RNA polymerase II transcription complex subunit 8

    3. XM_011240659.4 → XP_011238961.1  mediator of RNA polymerase II transcription subunit 8 isoform X2

      UniProtKB/TrEMBL
      Q9CY05
      Conserved Domains (1) summary
      pfam10232
      Location:1 → 176
      Med8; Mediator of RNA polymerase II transcription complex subunit 8
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9D7W5.1 GenPept UniProtKB/Swiss-Prot:Q9D7W5

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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