robo4 roundabout, axon guidance receptor, homolog 4 (Drosophila) [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: robo4provided by ZNC
Official Full Name: roundabout, axon guidance receptor, homolog 4 (Drosophila)provided by ZNC
Primary source: ZFIN:ZDB-GENE-020809-1
See related: Ensembl:ENSDARG00000009387 AllianceGenome:ZFIN:ZDB-GENE-020809-1
Gene type: protein coding
RefSeq status: MODEL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: sc:d0823; wu:fa16f09
Summary: Predicted to enable cell-cell adhesion mediator activity. Acts upstream of or within several processes, including plasma membrane bounded cell projection assembly; positive regulation of Rac protein signal transduction; and positive regulation of cell-matrix adhesion. Located in plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; neural tube; optic cup; and vestibuloauditory system. Human ortholog(s) of this gene implicated in aortic valve disease 3. Orthologous to human ROBO4 (roundabout guidance receptor 4). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 10

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	10	NC_007121.7 (31244615..31282033)
105	previous assembly	GRCz10 (GCF_000002035.5)	10	NC_007121.6 (31357688..31395455)

Chromosome 10 - NC_007121.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sox7 and Sox18-mediated transcriptional regulation of Robo4 is important in the developing embryonic vasculature
Title: Sox factors transcriptionally regulate ROBO4 gene expression in developing vasculature in zebrafish.
robo4 is essential for coordinated symmetric and directed sprouting of intersomitic vessels.
Title: roundabout4 is essential for angiogenesis in vivo.
Robo4 mediates attraction-signaling mechanisms through Rho GTPases in vertebrate vascular guidance
Title: Robo4 signaling in endothelial cells implies attraction guidance mechanisms.

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General gene information

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Markers

Ndufb11 (e-PCR), detects polymorphism
- UniSTS:498527

fa16f09.s1 (e-PCR)
- UniSTS:202404

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables cell-cell adhesion mediator activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	IBA Inferred from Biological aspect of Ancestor more info
involved_in dendrite self-avoidance	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within endothelial cell migration	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within filopodium assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in homophilic cell adhesion via plasma membrane adhesion molecules	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lamellipodium assembly	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of Rac protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of Rac protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
NOT acts_upstream_of_or_within positive regulation of Rho protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cell-matrix adhesion	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of small GTPase mediated signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of small GTPase mediated signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: roundabout homolog 4

Names: magic roundabout

NCBI Reference Sequences (RefSeq)

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RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

NC_007121.7 Reference GRCz11 Primary Assembly

Range

31244615..31282033

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_684163.9 → XP_689255.3 roundabout homolog 4 isoform X1

UniProtKB/TrEMBL

A0A8N7UV64, F1RE66

Related

ENSDARP00000112841.1, ENSDART00000145562.2

Conserved Domains (6) summary

cd05724
Location:177 → 261

Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:70 → 169

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

smart00060
Location:373 → 448

FN3; Fibronectin type 3 domain

cd00063
Location:472 → 560

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:175 → 261

I-set; Immunoglobulin I-set domain

cl11960
Location:282 → 350

Ig; Immunoglobulin domain
XM_005172710.5 → XP_005172767.1 roundabout homolog 4 isoform X2

UniProtKB/TrEMBL

A0A8M2BK13

Conserved Domains (6) summary

cd05724
Location:136 → 220

Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd07693
Location:29 → 128

Ig1_Robo; First immunoglobulin (Ig)-like domain in Robo (roundabout) receptors and similar proteins

smart00060
Location:332 → 407

FN3; Fibronectin type 3 domain

cd00063
Location:431 → 519

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:134 → 220

I-set; Immunoglobulin I-set domain

cl11960
Location:241 → 309

Ig; Immunoglobulin domain