Piwil1 piwi-like RNA-mediated gene silencing 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Piwil1provided by MGI
Official Full Name: piwi-like RNA-mediated gene silencing 1provided by MGI
Primary source: MGI:MGI:1928897
See related: Ensembl:ENSMUSG00000029423 AllianceGenome:MGI:1928897
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MIWI
Summary: Enables several functions, including RNA binding activity; mRNA cap binding complex binding activity; and protein kinase binding activity. Involved in regulatory ncRNA-mediated gene silencing and sperm DNA condensation. Located in chromatoid body; dense body; and nucleus. Is expressed in several structures, including central nervous system; genitourinary system; hemolymphoid system gland; and lung. Orthologous to human PIWIL1 (piwi like RNA-mediated gene silencing 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward testis adult (RPKM 111.5) See more
Orthologs: human all
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Genomic context

Location:: 5 G1.3; 5 67.86 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (128813135..128832538)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (128736071..128755474)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

piRNA loading triggers MIWI translocation from the intermitochondrial cement to chromatoid body during mouse spermatogenesis.
Title: piRNA loading triggers MIWI translocation from the intermitochondrial cement to chromatoid body during mouse spermatogenesis.
Regulation of Miwi-mediated mRNA stabilization by Ck137956/Tssa is essential for male fertility.
Title: Regulation of Miwi-mediated mRNA stabilization by Ck137956/Tssa is essential for male fertility.
Noncanonical functions of PIWIL1/piRNAs in animal male germ cells and human diseasesdagger.
Title: Noncanonical functions of PIWIL1/piRNAs in animal male germ cells and human diseases†.
Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression.
Title: Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression.
MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.
Title: MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.
The MIWI-piRNA complex may play a role in Schwann cell responses to nerve injury.
Title: Downregulation MIWI-piRNA regulates the migration of Schwann cells in peripheral nerve injury.
MIWI/piRNA activates mRNA translation via imperfect base-pairing interactions.
Title: A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.
Data show that ubiquitin-like, containing PHD and RING finger domains, 1 protein (UHRF1) interacts with protein arginine N-methyltransferase 5 (PRMT5) to regulate the repressive histone arginine modifications, and cooperates with the PIWI pathway during spermatogenesis.
Title: UHRF1 suppresses retrotransposons and cooperates with PRMT5 and PIWI proteins in male germ cells.
we show that mitochondria-anchored TDRKH controls multiple steps of pachytene piRNA biogenesis in mice. TDRKH specifically recruits MIWI, but not MILI, to engage the piRNA pathway. It is required for the production of the entire MIWI-bound piRNA population and enables trimming of MILI-bound piRNAs.
Title: Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis.
PIWIL1 augments protein translation with PABPC1 in the presence of 3'-UTRs of post-meiotic mRNAs. While both the N-terminal RNA recognition motif (RRM) domain and the central linker region of PABPC1 stimulate translation, only the PIWI Argonaute and Zwille (PAZ) domain of PIWIL1 positively affects translation of reporter mRNAs.
Title: Domain-functional analyses of PIWIL1 and PABPC1 indicate their synergistic roles in protein translation via 3'-UTRs of meiotic mRNAs.

Submit: New GeneRIF Correction See all GeneRIFs (32)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4) 1 citation
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Piwil1 (e-PCR), detects polymorphism
- UniSTS:479019

UniSTS:498764 (e-PCR)
- UniSTS:498764

Piwil1 (e-PCR), detects polymorphism
- UniSTS:498764

NoName (e-PCR)
- UniSTS:548221

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA cap binding complex binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables piRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables piRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables piRNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pachytene	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pachytene	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in piRNA-mediated gene silencing by mRNA destabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in piRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytoplasmic translation	NAS Non-traceable Author Statement more info	PubMed
involved_in regulatory ncRNA-mediated gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulatory ncRNA-mediated gene silencing	ISS Inferred from Sequence or Structural Similarity more info
involved_in sperm DNA condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatid development	ISO Inferred from Sequence Orthology more info
involved_in spermatogenesis	EXP Inferred from Experiment more info	PubMed
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transposable element silencing by piRNA-mediated heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
located_in P granule	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatoid body	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in dense body	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: piwi-like protein 1

Names: piwi-like homolog 1

NP_067286.1

EC 3.1.26.-

XP_006504381.1

EC 3.1.26.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021311.3 → NP_067286.1 piwi-like protein 1

See identical proteins and their annotated locations for NP_067286.1

Status: PROVISIONAL

Source sequence(s)

AC111089

Consensus CDS

CCDS19690.1

UniProtKB/Swiss-Prot

A1L324, A1L325, Q6NXX0, Q9JMB7

Related

ENSMUSP00000083222.4, ENSMUST00000086056.8

Conserved Domains (3) summary

smart00949
Location:278 → 416

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:402 → 845

Piwi_piwi-like_Euk; PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related ...

pfam05831
Location:16 → 107

GAGE; GAGE protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

128813135..128832538

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006504318.2 → XP_006504381.1 piwi-like protein 1 isoform X1

See identical proteins and their annotated locations for XP_006504381.1

UniProtKB/Swiss-Prot

A1L324, A1L325, Q6NXX0, Q9JMB7

Related

ENSMUSP00000142386.2, ENSMUST00000195959.2

Conserved Domains (3) summary

smart00949
Location:278 → 416

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:402 → 845

Piwi_piwi-like_Euk; PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related ...

pfam05831
Location:16 → 107

GAGE; GAGE protein