AP-2alpha Adaptor Protein complex 2, alpha subunit [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: AP-2alphaprovided by FlyBase
Official Full Name: Adaptor Protein complex 2, alpha subunitprovided by FlyBase
Primary source: FLYBASE:FBgn0264855
Locus tag: Dmel_CG4260
See related: AllianceGenome:FB:FBgn0264855
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: ada; Ada; alpha; alpha-ada; alpha-Ada; alpha-adaptin; alpha-Adaptin; Alpha-Adaptin; alphaada; alphaAda; AP-2; Ap-2alpha; AP2; AP2alpha; apl3; CG31654; cg4260; CG4260; D-Ada; D-alphaAda; dAP-2a; Dmel\CG4260; l(2)06694; l(2)SH0460; l(2)SH2 0460; MENE (2L)-A; MENE(2L)-A
Summary: Enables clathrin adaptor activity. Involved in several processes, including endocytosis; follicle cell of egg chamber development; and mitotic cleavage furrow ingression. Located in clathrin-coated pit and plasma membrane. Part of AP-2 adaptor complex. Is expressed in several structures, including embryonic/larval midgut primordium; ganglia; garland cell; germ layer; and presumptive embryonic/larval system. Orthologous to human AP2A1 (adaptor related protein complex 2 subunit alpha 1) and AP2A2 (adaptor related protein complex 2 subunit alpha 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 21C2-21C2; 2-0.7 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (408030..414777, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (408030..414777, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Prd1, alpha-Adaptin, and Unc-104 act in a common pathway to promote dendrite pruning by down-regulating Neuroglian protein.
Title: Prd1 associates with the clathrin adaptor α-Adaptin and the kinesin-3 Imac/Unc-104 to govern dendrite pruning in Drosophila.
The adaptor protein 2 (AP-2) complex binds to a motif in the cytoplasmic tail of Crumbs that overlaps with the binding site of Stardust, a protein known to stabilize Crumbs on the surface.
Title: AP-2-complex-mediated endocytosis of Drosophila Crumbs regulates polarity by antagonizing Stardust.
Interaction of Notch signaling modulator Numb with alpha-Adaptin regulates endocytosis of Notch pathway components and cell fate determination of neural stem cells.
Title: Interaction of Notch signaling modulator Numb with α-Adaptin regulates endocytosis of Notch pathway components and cell fate determination of neural stem cells.
Numb polarizes the distribution of alpha-Adaptin, involved in receptor-mediated endocytosis. Alpha-Adaptin mutations not binding to Numb don't localize asymmetrically and cause defects in asymmetric cell division.
Title: The endocytic protein alpha-Adaptin is required for numb-mediated asymmetric cell division in Drosophila.
Numb and alpha-Adaptin regulate Sanpodo endocytosis to specify cell fate in Drosophila external sensory organs.
Title: Numb and alpha-Adaptin regulate Sanpodo endocytosis to specify cell fate in Drosophila external sensory organs.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0349 (e-PCR)
- UniSTS:12585

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables clathrin adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables clathrin adaptor activity	IEA Inferred from Electronic Annotation more info
enables clathrin adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in asymmetric cell division	TAS Traceable Author Statement more info	PubMed
involved_in clathrin-dependent endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in clathrin-dependent endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
involved_in mitotic cleavage furrow ingression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of signal transduction by receptor internalization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IDA Inferred from Direct Assay more info	PubMed
involved_in receptor internalization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle transport	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of AP-2 adaptor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of AP-2 adaptor complex	IEA Inferred from Electronic Annotation more info
part_of AP-2 adaptor complex	IMP Inferred from Mutant Phenotype more info	PubMed
located_in clathrin-coated pit	IDA Inferred from Direct Assay more info	PubMed
part_of membrane coat	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: adaptor protein complex 2, alpha subunit

Names: AP-2alpha-PA; AP-2alpha-PB; Alpha-adaptin; CG4260-PA; CG4260-PB; alpha adaptin; alphaAdaptin; clathrin AP-2 complex; lethal (2) SH0460

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

408030..414777 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057471.6 → NP_476819.2 adaptor protein complex 2, alpha subunit, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476819.2

Status: REVIEWED

UniProtKB/Swiss-Prot

B3DN51, O01937, P91926, Q7KTZ5, Q86P64, Q9VPP4

Related

FBpp0088490

Conserved Domains (3) summary

smart00809
Location:718 → 827

Alpha_adaptinC2; Adaptin C-terminal domain

pfam01602
Location:28 → 591

Adaptin_N; Adaptin N terminal region

pfam02296
Location:827 → 935

Alpha_adaptin_C; Alpha adaptin AP2, C-terminal domain
NM_205885.2 → NP_995607.1 adaptor protein complex 2, alpha subunit, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995607.1

Status: REVIEWED

UniProtKB/TrEMBL

E8NH12

Conserved Domains (3) summary

smart00809
Location:730 → 839

Alpha_adaptinC2; Adaptin C-terminal domain

pfam01602
Location:40 → 598

Adaptin_N; Adaptin N terminal region

pfam02296
Location:839 → 947

Alpha_adaptin_C; Alpha adaptin AP2, C-terminal domain