Ksr1 kinase suppressor of ras 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ksr1provided by MGI
Official Full Name: kinase suppressor of ras 1provided by MGI
Primary source: MGI:MGI:105051
See related: Ensembl:ENSMUSG00000018334 AllianceGenome:MGI:105051
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: hb; Ksr; RSU2; mKSR1; B-KSR1; D11Bhm183e; D11Bhm184e
Summary: Enables several functions, including 14-3-3 protein binding activity; ATP binding activity; and Hsp90 protein binding activity. Involved in intracellular signaling cassette; regulation of MAP kinase activity; and regulation of cell population proliferation. Acts upstream of or within positive regulation of MAPK cascade. Predicted to be located in cytosol and intracellular membrane-bounded organelle. Predicted to be part of protein-containing complex. Predicted to be active in endoplasmic reticulum and membrane. Is expressed in several structures, including brain; genitourinary system; gut; hemolymphoid system gland; and lung. Human ortholog(s) of this gene implicated in breast adenocarcinoma. Orthologous to human KSR1 (kinase suppressor of ras 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in large intestine adult (RPKM 15.8), small intestine adult (RPKM 14.1) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B5; 11 46.74 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (78904266..79037357, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (79013440..79146533, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Calmodulin influences MAPK signaling by binding KSR1.
Title: Calmodulin influences MAPK signaling by binding KSR1.
DiRas3 binds to KSR1 independently of its interaction with activated Ras and RAF.
Title: Stabilization of C-RAF:KSR1 complex by DiRas3 reduces availability of C-RAF for dimerization with B-RAF.
praja2 regulates KSR1 stability and mitogenic signaling.
Title: praja2 regulates KSR1 stability and mitogenic signaling.
These results suggest that Rap1 activation of ERKs requires PKA phosphorylation and KSR binding.
Title: Phosphorylation of Rap1 by cAMP-dependent Protein Kinase (PKA) Creates a Binding Site for KSR to Sustain ERK Activation by cAMP.
Data show that caveolin-1 is necessary for optimal KSR1-dependent ERK activation by growth factors and oncogenic Ras.
Title: Caveolin-1 is required for kinase suppressor of Ras 1 (KSR1)-mediated extracellular signal-regulated kinase 1/2 activation, H-RasV12-induced senescence, and transformation.
propose that phosphorylation of Tyr(728) may regulate the transition between the scaffolding and the catalytic function of KSR1 serving as a control point used to fine-tune cellular responses
Title: Tyr728 in the kinase domain of the murine kinase suppressor of RAS 1 regulates binding and activation of the mitogen-activated protein kinase kinase.
Data indicate that phosphorylation of BRAF by AMPK disrupts its association with KSR1.
Title: Phosphorylation of BRAF by AMPK impairs BRAF-KSR1 association and cell proliferation.
Despite its role in mTORC1 activation, KSR1 is not required in vivo for mTOR-dependent T cell differentiation.
Title: Kinase suppressor of Ras 1 is not required for the generation of regulatory and memory T cells.
Ksr1 plays a dispensable role in lipopolysaccharide (LPS)-induced ERK activation in alveolar macrophages and does not contribute to the development of acute lung injury in the LPS model.
Title: Role of kinase suppressor of ras-1 in lipopolysaccharide-induced acute lung injury.
the atypical C1 domain, the CC-SAM domain is required to target KSR-1 to the plasma membrane.
Title: A CC-SAM, for coiled coil-sterile α motif, domain targets the scaffold KSR-1 to specific sites in the plasma membrane.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW492498 (e-PCR)
- UniSTS:180265

U43585 (e-PCR)
- UniSTS:125750

D11Bhm89 (e-PCR), detects polymorphism
- UniSTS:141696

D11Bhm90 (e-PCR), detects polymorphism
- UniSTS:141698

D11Bhm91 (e-PCR), detects polymorphism
- UniSTS:141699

D11Bhm92 (e-PCR), detects polymorphism
- UniSTS:141700

D11Bhm93 (e-PCR), detects polymorphism
- UniSTS:141701

D11Bhm94 (e-PCR), detects polymorphism
- UniSTS:141702

D11Bhm95 (e-PCR), detects polymorphism
- UniSTS:141703

D11Bhm96 (e-PCR), detects polymorphism
- UniSTS:141704

D11Bhm97 (e-PCR), detects polymorphism
- UniSTS:141705

D11Bhm98 (e-PCR), detects polymorphism
- UniSTS:141706

Ksr1 (e-PCR), detects polymorphism
- UniSTS:536479

D11Mit91 (e-PCR), detects polymorphism
- UniSTS:126639

D11Mit96 (e-PCR), detects polymorphism
- UniSTS:126644

D11Seg44 (e-PCR), detects polymorphism
- UniSTS:141820

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 14-3-3 protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables 14-3-3 protein binding	ISO Inferred from Sequence Orthology more info
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Hsp90 protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables MAP-kinase scaffold activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP-kinase scaffold activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables mitogen-activated protein kinase kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables mitogen-activated protein kinase kinase binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	ISO Inferred from Sequence Orthology more info	PubMed
enables protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein-folding chaperone binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Ras protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in regulation of MAP kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISO Inferred from Sequence Orthology more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed
located_in ruffle membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: kinase suppressor of Ras 1

Names: protein Hb

NP_001335136.1

EC 2.7.11.1

NP_038599.1

EC 2.7.11.1

XP_006532397.1

EC 2.7.11.1

XP_006532398.1

EC 2.7.11.1

XP_011247072.1

EC 2.7.11.1

XP_011247073.1

EC 2.7.11.1

XP_011247074.1

EC 2.7.11.1

XP_011247075.1

EC 2.7.11.1

XP_011247076.1

EC 2.7.11.1

XP_011247077.1

EC 2.7.11.1

XP_036012252.1

EC 2.7.11.1

XP_036012253.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001348207.1 → NP_001335136.1 kinase suppressor of Ras 1 isoform 2

Status: VALIDATED

Source sequence(s)

AK137532, CX204281, U43585

Consensus CDS

CCDS88203.1

UniProtKB/TrEMBL

A0A2I3BR95, Q3UV75

Related

ENSMUSP00000154385.2, ENSMUST00000226282.2

Conserved Domains (3) summary

cd00029
Location:252 → 294

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:480 → 758

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:42 → 84

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1
NM_013571.3 → NP_038599.1 kinase suppressor of Ras 1 isoform 1

See identical proteins and their annotated locations for NP_038599.1

Status: VALIDATED

Source sequence(s)

AK137532, AK164833, CX204281, U43585

Consensus CDS

CCDS25117.1

UniProtKB/Swiss-Prot

Q61097, Q61648, Q78DX8

UniProtKB/TrEMBL

Q3UV75

Related

ENSMUSP00000018478.5, ENSMUST00000018478.11

Conserved Domains (4) summary

cd00029
Location:334 → 376

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:562 → 840

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam07714
Location:563 → 827

Pkinase_Tyr; Protein tyrosine kinase

pfam13543
Location:39 → 166

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

78904266..79037357 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011248771.4 → XP_011247073.1 kinase suppressor of Ras 1 isoform X2

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd00029
Location:334 → 376

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:562 → 840

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:39 → 166

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1
XM_011248770.4 → XP_011247072.1 kinase suppressor of Ras 1 isoform X1

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd00029
Location:334 → 376

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:576 → 854

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:39 → 166

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1
XM_036156359.1 → XP_036012252.1 kinase suppressor of Ras 1 isoform X3

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd14152
Location:576 → 854

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:39 → 166

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1

cd20872
Location:332 → 378

C1_KSR1; protein kinase C conserved region 1 (C1 domain) found in kinase suppressor of Ras 1 (KSR1) and similar proteins
XM_036156360.1 → XP_036012253.1 kinase suppressor of Ras 1 isoform X7

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd14152
Location:562 → 840

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:39 → 166

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1

cd20872
Location:332 → 378

C1_KSR1; protein kinase C conserved region 1 (C1 domain) found in kinase suppressor of Ras 1 (KSR1) and similar proteins
XM_006532334.5 → XP_006532397.1 kinase suppressor of Ras 1 isoform X5

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd00029
Location:334 → 376

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:576 → 854

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:39 → 166

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1
XM_006532335.5 → XP_006532398.1 kinase suppressor of Ras 1 isoform X6

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd00029
Location:334 → 376

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:576 → 854

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:39 → 166

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1
XM_011248775.3 → XP_011247077.1 kinase suppressor of Ras 1 isoform X4

See identical proteins and their annotated locations for XP_011247077.1

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (2) summary

cd00029
Location:199 → 241

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:441 → 719

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1
XM_011248772.3 → XP_011247074.1 kinase suppressor of Ras 1 isoform X4

See identical proteins and their annotated locations for XP_011247074.1

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (2) summary

cd00029
Location:199 → 241

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:441 → 719

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1
XM_011248774.4 → XP_011247076.1 kinase suppressor of Ras 1 isoform X9

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd00029
Location:220 → 262

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:462 → 740

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:1 → 52

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1
XM_011248773.4 → XP_011247075.1 kinase suppressor of Ras 1 isoform X8

UniProtKB/TrEMBL

Q3UV75

Conserved Domains (3) summary

cd00029
Location:252 → 294

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd14152
Location:494 → 772

STKc_KSR1; Catalytic domain of the Serine/Threonine Kinase, Kinase Suppressor of Ras 1

pfam13543
Location:42 → 84

KSR1-SAM; SAM like domain present in kinase suppressor RAS 1