ETT Transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-like protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: ETT
Official Full Name: Transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-like protein
Primary source: TAIR:AT2G33860
Locus tag: AT2G33860
See related: Araport:AT2G33860
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARF3; AUXIN RESPONSE TRANSCRIPTION FACTOR 3; ETTIN; T1B8.30; T1B8_30
Summary: ettin (ett) mutations have pleiotropic effects on Arabidopsis flower development, causing increases in perianth organ number, decreases in stamen number and anther formation, and apical-basal patterning defects in the gynoecium. The ETTIN gene encodes a protein with homology to DNA binding proteins which bind to auxin response elements. ETT transcript is expressed throughout stage 1 floral meristems and subsequently resolves to a complex pattern within petal, stamen and carpel primordia. ETT probably functions to impart regional identity in floral meristems that affects perianth organ number spacing, stamen formation, and regional differentiation in stamens and the gynoecium. During stage 5, ETT expression appears in a ring at the top of the floral meristem before morphological appearance of the gynoecium, consistent with the proposal that ETT is involved in prepatterning apical and basal boundaries in the gynoecium primordium. It is a target of the ta-siRNA tasiR-ARF.
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Genomic context

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See ETT in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 10

Sequence:: Chromosome: 2; NC_003071.7 (14325206..14328978, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Immediate targets of ETTIN suggest a key role for pectin methylesterase inhibitors in the control of Arabidopsis gynecium development.
Title: Immediate targets of ETTIN suggest a key role for pectin methylesterase inhibitors in the control of Arabidopsis gynecium development.
High-throughput library screening identified an almost full-length soluble C-terminal ETT specific domain in Arabidopsis ETTIN protein that did not bind auxin directly but exhibited a dose-dependent response in a yeast two-hybrid system against the Arabidopsis INDEHISCENT (IND) transcription factor. Circular dichroism confirmed the domain was disordered.
Title: Auxin sensing is a property of an unstructured domain in the Auxin Response Factor ETTIN of Arabidopsis thaliana.
ETT controls the elongation of the valve tissues of the gynoecium through the positive regulation of pectin methylesterase (PME) activity in the cell wall.
Title: Evidence for the Regulation of Gynoecium Morphogenesis by ETTIN via Cell Wall Dynamics.
Epistatic interaction of CTR1 and ALF3 genes in Arabidopsis roots has been reported. [ALF3]
Title: Features inheritance of root system Arabidopsis thaliana (L.) Heynh. the interaction of genes CTR1 AND ALF3, NPH4 and IAR2.
ETT controls developmental processes in the Arabidopsis shoot through its auxin-sensing property.
Title: Auxin-Induced Modulation of ETTIN Activity Orchestrates Gene Expression in Arabidopsis.
Auxin Response Factor 3 (ARF3) was identified as a mediator of cross-talk between self-incompatibility signaling and pistil development.
Title: Non-cell-autonomous regulation of crucifer self-incompatibility by Auxin Response Factor ARF3.
ATS and ETT were found to share an overlapping expression pattern during Arabidopsis ovule development and loss of either gene resulted in congenital fusion of the integuments and altered seed morphology.
Title: ETTIN (ARF3) physically interacts with KANADI proteins to form a functional complex essential for integument development and polarity determination in Arabidopsis.
Mutations in ETTIN were isolated as second site suppressors and found to ameliorate ectopic KANADI activity throughout the plant as well. Mutant phenotypes of ett are restricted to flowers.
Title: Auxin response factors mediate Arabidopsis organ asymmetry via modulation of KANADI activity.
Mutations delay the expression of adult traits, demonstrating that these genes have non-redundant roles in shoot maturation.
Title: Trans-acting siRNA-mediated repression of ETTIN and ARF4 regulates heteroblasty in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Miscellaneous features

Loc: c14328976-14328698 AT2G33860.uORF1

NM_001336475.1

Supported by ESTs:

NM_128946.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables DNA-binding transcription factor activity	TAS Traceable Author Statement more info	PubMed
enables chromatin DNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription cis-regulatory region binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within abaxial cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within auxin metabolic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within floral meristem determinacy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of response to water deprivation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within response to auxin	ISS Inferred from Sequence or Structural Similarity more info	PubMed
acts_upstream_of_or_within vegetative phase change	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within vegetative phase change	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: Transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-like protein

NP_180942.1

ETTIN (ETT); FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: response to auxin stimulus, vegetative phase change, regulation of transcription, DNA-dependent, abaxial cell fate specification, auxin metabolic process; LOCATED IN: nucleus; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Transcriptional factor B3 (InterPro:IPR003340), Auxin response factor (InterPro:IPR010525); BEST Arabidopsis thaliana protein match is: auxin response factor 4 (TAIR:AT5G60450.1); Has 1211 Blast hits to 1200 proteins in 55 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 0; Plants - 1202; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

14325206..14328978 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_128946.4 → NP_180942.1 Transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_180942.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O23661, O23670, O81087, Q33DU9

UniProtKB/TrEMBL

A0A178VTM6, A0A5S9X3R2, A0A654F998

Conserved Domains (2) summary

pfam02362
Location:159 → 261

B3; B3 DNA binding domain

pfam06507
Location:285 → 366

Auxin_resp; Auxin response factor
NM_001336475.1 → NP_001324363.1 Transcriptional factor B3 family protein / auxin-responsive factor AUX/IAA-like protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AZW3