MTO1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: MTO1
Official Full Name: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein
Primary source: TAIR:AT3G01120
Locus tag: AT3G01120
See related: Araport:AT3G01120
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: A. THALIANA CYSTATHIONINE GAMMA-SYNTHASE 1; A. thaliana cystathionine gamma-synthetase 1; AtCGS1; AtCYS1; ATCYS1; CGS; CGS1; CYSTATHIONINE GAMMA-SYNTHASE; CYSTATHIONINE GAMMA-SYNTHASE 1; CYSTATHIONINE-GAMMA-SYNTHASE; METHIONINE OVERACCUMULATION 1; T4P13.19; T4P13_19
Summary: encodes a cystathionine gamma-synthase, which performs the first committed step in methionine biosynthesis. A conserved motif of 13 amino acids in the first exon is required for posttranscriptional autoregulation. This enzyme shares the same substrate as threonine synthase (TS) and its absence transcriptionally affects 8 genes in the genome.
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Genomic context

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See MTO1 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 11

Sequence:: Chromosome: 3; NC_003074.8 (38759..42159, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cystathionine gamma-synthase expression in seeds alters metabolic and DNA methylation profiles in Arabidopsis.
Title: Cystathionine γ-synthase expression in seeds alters metabolic and DNA methylation profiles in Arabidopsis.
Seed-specific repression of AtCGS in RNA interference seeds triggered the induction of genes operating in the SMM cycle of rosette leaves.
Title: Repression of CYSTATHIONINE γ-SYNTHASE in Seeds Recruits the S-Methylmethionine Cycle.
Cystathione-gamma-synthase stimulates metabolic and transcriptomic responses associated with desiccation stress in seeds.
Title: Seed-specific expression of a feedback-insensitive form of CYSTATHIONINE-γ-SYNTHASE in Arabidopsis stimulates metabolic and transcriptomic responses associated with desiccation stress.
Report shows that the poly(A) tail of the full-length CGS1 mRNA showed an apparent increase from 50-80 nucleotides (nt)to 140-150 nt after the induction of CGS1 mRNA degradation.
Title: A halt in poly(A) shortening during S-adenosyl-L-methionine-induced translation arrest in CGS1 mRNA of Arabidopsis thaliana.
Data indicate that stacked ribosomes play in the control of cystathionine gamma-synthase CGS1 mRNA degradation.
Title: Ribosomes in a stacked array: elucidation of the step in translation elongation at which they are stalled during S-adenosyl-L-methionine-induced translation arrest of CGS1 mRNA.
Light and sucrose up-regulate the expression level of cystathionine gamma-synthase.
Title: Light and sucrose up-regulate the expression level of Arabidopsis cystathionine γ-synthase, the key enzyme of methionine biosynthesis pathway.
These results show that S-adenosyl-L-methionine induces the stacking of ribosomes on CGS1 mRNA and that multiple mRNA degradation sites probably correspond to each stacked ribosome.
Title: Ribosome stacking defines CGS1 mRNA degradation sites during nascent peptide-mediated translation arrest.
CGS1 mRNA degradation involves a plant-specific mechanism.
Title: Nascent peptide-mediated translation elongation arrest of Arabidopsis thaliana CGS1 mRNA occurs autonomously.
The results of this study suggest that a metabolic effector, to date unidentified, can modulate CGS activity in vivo through an interaction with the N-terminal domain of the enzyme and that removal of this domain can suppress this regulation.
Title: Regulation of one-carbon metabolism in Arabidopsis: the N-terminal regulatory domain of cystathionine gamma-synthase is cleaved in response to folate starvation.
The transcript of the N-terminus region deleted form of CGS is insensitive to methionine application and its expression may be important for maintaining methionine metabolism even in the presence of a high level of methionine.
Title: An in vivo internal deletion in the N-terminus region of Arabidopsis cystathionine gamma-synthase results in CGS expression that is insensitive to methionine.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_110977.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables cystathionine gamma-synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables cystathionine gamma-synthase activity	IEA Inferred from Electronic Annotation more info
enables cystathionine gamma-synthase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables pyridoxal phosphate binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within methionine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within selenium compound metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in transsulfuration	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast stroma	HDA more info	PubMed

General protein information

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Preferred Names: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein

NP_186761.1

METHIONINE OVERACCUMULATION 1 (MTO1); FUNCTIONS IN: cystathionine gamma-synthase activity; INVOLVED IN: methionine biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cys/Met metabolism, pyridoxal phosphate-dependent enzyme (InterPro:IPR000277), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (InterPro:IPR015422); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT1G33320.1); Has 21364 Blast hits to 20702 proteins in 2452 species: Archae - 220; Bacteria - 11705; Metazoa - 241; Fungi - 842; Plants - 284; Viruses - 13; Other Eukaryotes - 8059 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

38759..42159 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_110977.3 → NP_186761.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_186761.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P55217, P92944, P93038, Q42550

UniProtKB/TrEMBL

A0A178VB36, A0A5S9X8A2, A0A7G2EL88

Conserved Domains (1) summary

pfam01053
Location:176 → 561

Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme