PAD3 Cytochrome P450 superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PAD3
Official Full Name: Cytochrome P450 superfamily protein
Primary source: TAIR:AT3G26830
Locus tag: AT3G26830
See related: Araport:AT3G26830
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: CYP71B15; PHYTOALEXIN DEFICIENT 3
Summary: Mutations in pad3 are defective in biosynthesis of the indole derived phytoalexin camalexin. Encodes a cytochrome P450 enzyme that catalyzes the conversion of dihydrocamalexic acid to camalexin.
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Genomic context

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See PAD3 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 2

Sequence:: Chromosome: 3; NC_003074.8 (9887917..9889791)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PAD3 is also required for induced resistance to green peach aphid, independently of BAK1 and reactive oxygen species production.
Title: The leucine-rich repeat receptor-like kinase BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 and the cytochrome P450 PHYTOALEXIN DEFICIENT3 contribute to innate immunity to aphids in Arabidopsis.
MKS1 interacts with the transcription factor WRKY33, which in turn controls the regulation of PAD3 and CYP71A13, two genes, required for proper resistance to B. cinerea.
Title: Constitutive expression of MKS1 confers susceptibility to Botrytis cinerea infection independent of PAD3 expression.
Indole-3-acetonitrile is derivatized to the intermediate Cys(IAN), which serves as substrate of the multifunctional cytochrome P450 enzyme CYP71B15.
Title: The multifunctional enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) converts cysteine-indole-3-acetonitrile to camalexin in the indole-3-acetonitrile metabolic network of Arabidopsis thaliana.
PHYTOALEXIN DEFICIENT3 (PAD3) is strongly up-regulated by both oligogalacturonides and Botrytis infection independently of salicylic acid, jasmonate, and ethylene.
Title: Resistance to Botrytis cinerea induced in Arabidopsis by elicitors is independent of salicylic acid, ethylene, or jasmonate signaling but requires PHYTOALEXIN DEFICIENT3.
PAD3 catalyzes the conversion of dihydrocamalexic acid to camalexin, the final step in camalexin biosynthesis.
Title: CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_113595.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables dihydrocamalexic acid decarboxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables monooxygenase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within camalexin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within camalexin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to fungus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within indole phytoalexin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of systemic acquired resistance	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to abscisic acid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to insect	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to water deprivation	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info
located_in endoplasmic reticulum lumen	IDA Inferred from Direct Assay more info
located_in intracellular membrane-bounded organelle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Cytochrome P450 superfamily protein

NP_189318.1

PHYTOALEXIN DEFICIENT 3 (PAD3); FUNCTIONS IN: dihydrocamalexic acid decarboxylase activity, monooxygenase activity, oxygen binding; INVOLVED IN: in 6 processes; LOCATED IN: endoplasmic reticulum, microsome; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 71, subfamily B, polypeptide 28 (TAIR:AT1G13090.1); Has 31909 Blast hits to 31706 proteins in 1646 species: Archae - 46; Bacteria - 3053; Metazoa - 11727; Fungi - 6529; Plants - 9477; Viruses - 0; Other Eukaryotes - 1077 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

9887917..9889791

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_113595.4 → NP_189318.1 Cytochrome P450 superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_189318.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q541X4, Q9LW27

UniProtKB/TrEMBL

A0A178V9Z3, A0A5S9XFY4, A0A654FB33

Conserved Domains (1) summary

cd11072
Location:59 → 474

CYP71-like; cytochrome P450 family 71 and similar cytochrome P450s