PIP2A plasma membrane intrinsic protein 2A [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PIP2A
Official Full Name: plasma membrane intrinsic protein 2A
Primary source: TAIR:AT3G53420
Locus tag: AT3G53420
See related: Araport:AT3G53420
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtPIP2;1; PIP2; PIP2;1; PLASMA MEMBRANE INTRINSIC PROTEIN 2; PLASMA MEMBRANE INTRINSIC PROTEIN 2;1; plasma membrane intrinsic protein 2A
Summary: a member of the plasma membrane intrinsic protein subfamily PIP2. localizes to the plasma membrane and exhibits water transport activity in Xenopus oocyte. expressed specifically in the vascular bundles and protein level increases slightly during leaf dev. When expressed in yeast cells can conduct hydrogen peroxide into those cells.
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Genomic context

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See PIP2A in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 5

Sequence:: Chromosome: 3; NC_003074.8 (19803473..19805730, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Arabidopsis plasma membrane intrinsic protein (AtPIP2;1) is implicated in a salinity conditional influence on seed germination.
Title: Arabidopsis plasma membrane intrinsic protein (AtPIP2;1) is implicated in a salinity conditional influence on seed germination.
ERAD-related E2 and E3 enzymes modulate the drought response by regulating the stability of PIP2 aquaporins.
Title: ERAD-related E2 and E3 enzymes modulate the drought response by regulating the stability of PIP2 aquaporins.
Phosphorylation of AtPIP2;1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H2O2 transport activities.
Title: Aquaporins facilitate hydrogen peroxide entry into guard cells to mediate ABA- and pathogen-triggered stomatal closure.
Transiently expressed RIN4, but not the plasma membrane (PM) protein aquaporin PIP2, recruits EXO70B1 to the plasma membrane (PM). Unlike EXO70B1, RIN4 does not recruit the core exocyst subunit SEC6 to the PM under these conditions.
Title: RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane.
PIP2;1 activity is regulated by by Ca(2+) and pH.
Title: Non-selective cation channel activity of aquaporin AtPIP2;1 regulated by Ca(2+) and pH.
Results identified 2 members of the receptor-like kinase (RLK) family (RKL1 (At1g48480) and Feronia (At3g51550)) differentially modulate PIP1;2 and PIP2;1 activity through distinct molecular mechanisms.
Title: Novel Aquaporin Regulatory Mechanisms Revealed by Interactomics.
study reveals that C-terminal phosphorylation of a water channel subunit, PIP2;1, is upregulated by an EIN3EIL1-independent ethylene signaling pathway and its water transport activity is enhanced by phosphorylation at the C-terminal S280 and S283 sites
Title: Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis.
This work supports a model whereby abscissic acid-triggered stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen peroxide, through OST1-dependent phosphorylation of PIP2;1 at Ser-121.
Title: Aquaporins Contribute to ABA-Triggered Stomatal Closure through OST1-Mediated Phosphorylation.
PIP2;1 molecules are heterogeneously distributed on the plasma membrane and that clathrin and membrane raft pathways cooperate to mediate the subcellular trafficking of PIP2;1.
Title: Single-molecule analysis of PIP2;1 dynamics and partitioning reveals multiple modes of Arabidopsis plasma membrane aquaporin regulation.
Co-expression of AtPIP2;1-GFP and AtPIP1;4-mCherry constructs suggested that ER-retained AtPIP2;1-GFP may interact with other PIPs to hamper their trafficking to the PM, thereby contributing to inhibition of root cell hydraulic conductivity
Title: Mechanisms and effects of retention of over-expressed aquaporin AtPIP2;1 in the endoplasmic reticulum.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001035774.1

Supported by ESTs:

NM_115202.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables mRNA binding	HDA more info	PubMed
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed
enables water channel activity	IDA Inferred from Direct Assay more info	PubMed
enables water channel activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within hydrogen peroxide transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to water deprivation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within water transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in plant-type vacuole	HDA more info	PubMed
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISM Inferred from Sequence Model more info
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: plasma membrane intrinsic protein 2A

NP_001030851.1

plasma membrane intrinsic protein 2A (PIP2A); FUNCTIONS IN: water channel activity; INVOLVED IN: response to water deprivation, response to salt stress, transport, hydrogen peroxide transmembrane transport, water transport; LOCATED IN: plasma membrane, vacuole, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Major intrinsic protein, conserved site (InterPro:IPR022357), Aquaporin (InterPro:IPR012269), Major intrinsic protein (InterPro:IPR000425); BEST Arabidopsis thaliana protein match is: plasma membrane intrinsic protein 2 (TAIR:AT2G37170.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_190910.1

plasma membrane intrinsic protein 2A (PIP2A); FUNCTIONS IN: water channel activity; INVOLVED IN: response to water deprivation, response to salt stress, transport, hydrogen peroxide transmembrane transport, water transport; LOCATED IN: plasma membrane, chloroplast, vacuole, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Major intrinsic protein, conserved site (InterPro:IPR022357), Aquaporin (InterPro:IPR012269), Major intrinsic protein (InterPro:IPR000425); BEST Arabidopsis thaliana protein match is: plasma membrane intrinsic protein 2 (TAIR:AT2G37170.1); Has 10900 Blast hits to 10884 proteins in 2233 species: Archae - 81; Bacteria - 5210; Metazoa - 1477; Fungi - 457; Plants - 2523; Viruses - 2; Other Eukaryotes - 1150 (source: NCBI BLink).