SR45 arginine/serine-rich 45 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: SR45
Official Full Name: arginine/serine-rich 45
Primary source: TAIR:AT1G16610
Locus tag: AT1G16610
See related: Araport:AT1G16610
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: arginine/serine-rich 45; ARGININE/SERINE-RICH PROTEIN SR45; AtSR45; F19K19.9; F19K19_9; RNPS1
Summary: Encodes SR45, a member of the highly conserved family of serine/arginine-rich (SR) proteins, which play key roles in pre-mRNA splicing and other aspects of RNA metabolism. SR45 is a spliceosome protein, interacts with SR33 and the U1-70K protein of the U1 snRNP. Also involved in plant sugar response. sr45-1 mutation confers hypersensitivity to glucose during early seedling growth.
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Genomic context

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See SR45 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 12

Sequence:: Chromosome: 1; NC_003070.9 (5675495..5678873, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Arabidopsis SR45 splicing factor bridges the splicing machinery and the exon-exon junction complex.
Title: The Arabidopsis SR45 splicing factor bridges the splicing machinery and the exon-exon junction complex.
The loss of SR45 results in enhanced sensitivity to salt stress and changes in expression and splicing of genes involved in regulating salt stress response.Only the long isoform (SR45.1) rescued the salt-sensitive phenotype as well as the altered gene expression and splicing patterns in the mutant.
Title: The Arabidopsis splicing regulator SR45 confers salt tolerance in a splice isoform-dependent manner.
Data demonstrate that SR45 regulates alternative splicing of the Arabidopsis 5PTase13 gene, which encodes an inositol polyphosphate 5-phosphatase previously shown to interact with and regulate the stability of SnRK1 in vitro, thus providing a mechanistic link between SR45 function and the modulation of degradation of the SnRK1 energy sensor in response to sugars.
Title: The Arabidopsis SR45 Splicing Factor, a Negative Regulator of Sugar Signaling, Modulates SNF1-Related Protein Kinase 1 Stability.
SR45 plays an unexpected role in mRNA processing of intronless genes, and numerous abscisic acid (ABA) signaling genes are targeted for regulation at the posttranscriptional level.
Title: Transcriptome-Wide Identification of RNA Targets of Arabidopsis SERINE/ARGININE-RICH45 Uncovers the Unexpected Roles of This RNA Binding Protein in RNA Processing.
SR45 recruits U1snRNP and U2AF to 5' and 3' splice sites, respectively, by interacting with pre-mRNA, U1-70K and U2AF(35) and modulates alternative splicing (AS).
Title: Interactions of SR45, an SR-like protein, with spliceosomal proteins and an intronic sequence: insights into regulated splicing.
Subcellular and subnuclear distribution of atSR45a and atSR30
Title: Subcellular and subnuclear distribution of high-light responsive serine/arginine-rich proteins, atSR45a and atSR30, in Arabidopsis thaliana.
DNA methylation establishment and maintenance defects present in sr45-1 mutants are enhanced in dcl3-1 mutant background, suggesting a synergistic cooperation between SR45 and DICER-LIKE3 (DCL3) in the RdDM pathway.
Title: The splicing factor SR45 affects the RNA-directed DNA methylation pathway in Arabidopsis.
SR45 is a negative regulator of sugar signaling during early seedling development and is involved in the repression of glucose-induced ABA accumulation and down-regulation of ABA biosynthesis
Title: The plant-specific SR45 protein negatively regulates glucose and ABA signaling during early seedling development in Arabidopsis.
The two alternatively spliced isoforms of SR$% have distinct biological functions.
Title: Two alternatively spliced isoforms of the Arabidopsis SR45 protein have distinct roles during normal plant development.
atSR45a proteins help to form the bridge between 5' and 3' splice sites in the spliceosome assembly.
Title: Plant-specific SR-related protein atSR45a interacts with spliceosomal proteins in plant nucleus.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001198085.1

Supported by ESTs:

NM_001332238.1

Supported by ESTs:

NM_001332239.1

Supported by ESTs:

NM_001332240.1

Supported by ESTs:

NM_001332241.1

Supported by ESTs:

NM_101523.4

Supported by ESTs:

NM_202115.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within RNA splicing	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within mRNA splicing, via spliceosome	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of alternative mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to abscisic acid	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sugar mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sugar mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nuclear speck	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	HDA more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: arginine/serine-rich 45

NP_001185014.1

arginine/serine-rich 45 (SR45); CONTAINS InterPro DOMAIN/s: RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: arginine/serine-rich protein-related (TAIR:AT1G79100.1).

NP_173107.1

arginine/serine-rich 45 (SR45); FUNCTIONS IN: protein binding, RNA binding; INVOLVED IN: sugar mediated signaling pathway, nuclear mRNA splicing, via spliceosome, RNA splicing; LOCATED IN: nucleoplasm, nuclear speck, nucleolus; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: arginine/serine-rich protein-related (TAIR:AT1G79100.1); Has 62425 Blast hits to 38843 proteins in 1486 species: Archae - 39; Bacteria - 6019; Metazoa - 27409; Fungi - 8585; Plants - 6560; Viruses - 1329; Other Eukaryotes - 12484 (source: NCBI BLink).

NP_973844.1

arginine/serine-rich 45 (SR45); FUNCTIONS IN: protein binding, RNA binding; INVOLVED IN: sugar mediated signaling pathway, nuclear mRNA splicing, via spliceosome, RNA splicing; LOCATED IN: nucleoplasm, nuclear speck, nucleolus; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: arginine/serine-rich protein-related (TAIR:AT1G79100.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

5675495..5678873 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_202115.3 → NP_973844.1 arginine/serine-rich 45 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_973844.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W7Y0, A0A5S9UQN2

Conserved Domains (2) summary

COG0724
Location:96 → 199

RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]

cd12365
Location:100 → 172

RRM_RNPS1; RNA recognition motif in RNA-binding protein with serine-rich domain 1 (RNPS1) and similar proteins
NM_001332238.1 → NP_001321127.1 arginine/serine-rich 45 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8APW3
NM_101523.4 → NP_173107.1 arginine/serine-rich 45 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_173107.1

Status: REVIEWED

UniProtKB/Swiss-Prot

F4I4H8, Q5E925, Q94AS1, Q9SEE9

Conserved Domains (2) summary

COG0724
Location:96 → 199

RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]

cd12365
Location:100 → 172

RRM_RNPS1; RNA recognition motif in RNA-binding protein with serine-rich domain 1 (RNPS1) and similar proteins
NM_001332239.1 → NP_001321128.1 arginine/serine-rich 45 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8APZ4
NM_001332241.1 → NP_001321126.1 arginine/serine-rich 45 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8APZ9
NM_001332240.1 → NP_001321125.1 arginine/serine-rich 45 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8APZ9
NM_001198085.1 → NP_001185014.1 arginine/serine-rich 45 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001185014.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W7W5

Conserved Domains (2) summary

COG0724
Location:96 → 199

RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]

cd12365
Location:100 → 172

RRM_RNPS1; RNA recognition motif in RNA-binding protein with serine-rich domain 1 (RNPS1) and similar proteins