keap1a kelch-like ECH-associated protein 1a [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: keap1aprovided by ZNC
Official Full Name: kelch-like ECH-associated protein 1aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-030131-556
See related: Ensembl:ENSDARG00000016132 AllianceGenome:ZFIN:ZDB-GENE-030131-556
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: keap1; wu:fb36f11
Summary: Predicted to enable ubiquitin-like ligase-substrate adaptor activity. Involved in protein ubiquitination and ubiquitin-dependent protein catabolic process. Acts upstream of or within cellular response to prostaglandin stimulus; cellular response to xenobiotic stimulus; and larval feeding behavior. Predicted to be located in inclusion body. Predicted to be part of Cul3-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. Is expressed in several structures, including digestive system; eye; gill; heart; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in gallbladder cancer. Orthologous to human KEAP1 (kelch like ECH associated protein 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: chromosome: 2

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	2	NC_007113.7 (41835025..41861040, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	2	NC_007113.6 (41985607..42011622, complement)

Chromosome 2 - NC_007113.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Genetic hyperactivation of Nrf2 causes larval lethality in Keap1a and Keap1b-double-knockout zebrafish.
Title: Genetic hyperactivation of Nrf2 causes larval lethality in Keap1a and Keap1b-double-knockout zebrafish.
The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling.
Title: The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling.
Generation and characterization of keap1a- and keap1b-knockout zebrafish.
Title: Generation and characterization of keap1a- and keap1b-knockout zebrafish.
Fucoxanthin Prevents 6-OHDA-Induced Neurotoxicity by Targeting Keap1.
Title: Fucoxanthin Prevents 6-OHDA-Induced Neurotoxicity by Targeting Keap1.
These results suggest that alpha,beta-unsaturated carbonyl entity and catechol moiety of 6S derivatives may react with the cysteine residues of Keap1, disrupting the Keap1-Nrf2 complex, thereby liberating and activating Nrf2. Our findings of natural product-derived Nrf2 activators lead to design options of potent Nrf2 activators for further optimization.
Title: Synthesis, evaluation, and metabolism of novel [6]-shogaol derivatives as potent Nrf2 activators.
study reports that the Keap1-Nrf2 system comprises discrete sensor sites, including the Keap1 cysteines Cys-151 and Cys-273, for a variety of Nrf2-activating compounds
Title: The antioxidant defense system Keap1-Nrf2 comprises a multiple sensing mechanism for responding to a wide range of chemical compounds.
mutation of either residual cysteine residue in Keap1a and Keap1b disrupted the ability of Keap1 to repress Nrf2, indicating that the presence of either Cys-273 or Cys-288 is sufficient for fish Keap1 molecules to fully function
Title: Molecular evolution of Keap1. Two Keap1 molecules with distinctive intervening region structures are conserved among fish.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI437003 (e-PCR)
- UniSTS:206599

fb36f11.x1 (e-PCR)
- UniSTS:190485

fi25a09.x1 (e-PCR)
- UniSTS:197097

fb36f11 (e-PCR)
- UniSTS:187117

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ubiquitin-like ligase-substrate adaptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cellular response to oxidative stress	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within cellular response to prostaglandin stimulus	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cellular response to xenobiotic stimulus	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within larval feeding behavior	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of autophagy	ISS Inferred from Sequence or Structural Similarity more info
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cul3-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in inclusion body	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: kelch-like ECH-associated protein 1A

Names: kelch-like ECH-associated protein 1; klhl19

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_182864.2 → NP_878284.2 kelch-like ECH-associated protein 1A

See identical proteins and their annotated locations for NP_878284.2

Status: PROVISIONAL

Source sequence(s)

BC117613

UniProtKB/Swiss-Prot

Q1ECZ2, Q8JIM0

Related

ENSDARP00000045762.6, ENSDART00000045763.7

Conserved Domains (3) summary

PHA03098
Location:54 → 571

PHA03098; kelch-like protein; Provisional

sd00038
Location:386 → 429

Kelch; KELCH repeat [structural motif]

cd18248
Location:33 → 156

BTB_POZ_KLHL19_KEAP1; BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ (poxvirus and zinc finger) domain found in Kelch-like ECH-associated protein 1 (KEAP1)