NIA1 nitrate reductase 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: NIA1
Official Full Name: nitrate reductase 1
Primary source: TAIR:AT1G77760
Locus tag: AT1G77760
See related: Araport:AT1G77760
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: GNR1; NITRATE REDUCTASE; nitrate reductase 1; NITRATE REDUCTASE 1; NR1; T32E8.9; T32E8_9
Summary: Encodes the cytosolic minor isoform of nitrate reductase (NR). Involved in the first step of nitrate assimilation, it contributes about 15% of the nitrate reductase activity in shoots. Similar to molybdopterin oxidoreductases at the N-terminus, and to FAD/NAD-binding cytochrome reductases at the C-terminus. Cofactors: FAD, heme iron (cytochrome B-557), and molybdenum-pterin.
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Genomic context

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See NIA1 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 4

Sequence:: Chromosome: 1; NC_003070.9 (29235721..29239484, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The NAC056 transcription factor confers freezing tolerance by positively regulating expression of CBFs and NIA1 in Arabidopsis.
Title: The NAC056 transcription factor confers freezing tolerance by positively regulating expression of CBFs and NIA1 in Arabidopsis.
Pseudomonas aeruginosa LasI-dependent plant growth promotion requires the host nitrate transceptor AtNRT1.1/CHL1 and the nitrate reductases NIA1 and NIA2.
Title: Pseudomonas aeruginosa LasI-dependent plant growth promotion requires the host nitrate transceptor AtNRT1.1/CHL1 and the nitrate reductases NIA1 and NIA2.
Study show that transcripts of both genes are present throughout the life cycle of Arabidopsis thaliana plants with NIA1 being predominantly active in leaves and NIA2 in meristematic tissues.
Title: Tissue-specific NIA1 and NIA2 expression in Arabidopsis thaliana.
Findings showed that each NR gene, NIA1 and NIA2, differentially contributes to reaching the optimum level of NO during the gravitropic response. In seedlings NIA1 accounts for 10%of the total NR activity. In roots, NIA1 is expressed at higher levels than NIA2.
Title: Nitrate reductase mediates nitric oxide-dependent gravitropic response in Arabidopsis thaliana roots.
Cell wall damage enhances cytokinin degradation rates through a NIA1/2-mediated process, leading to attenuation of cell cycle gene expression.
Title: Cell wall integrity modulates Arabidopsis thaliana cell cycle gene expression in a cytokinin- and nitrate reductase-dependent manner.
Genetic and biochemical evidences not only show that MEK1-MPK6 cascade, AtRBOHD/F-dependent H2O2 and NIA1-dependent NO are all involved in dark-induced stomatal closure in Arabidopsis, also indicate that MEK1-MPK6 cascade functions via working downstream of H2O2 and upstream of NO.
Title: Role and interrelationship of MEK1-MPK6 cascade, hydrogen peroxide and nitric oxide in darkness-induced stomatal closure.
mutating NIA1 and NIA2 impaired nearly all the key components of guard cell abscissic acid signaling pathway in Arabidopsis.
Title: Loss of nitrate reductases NIA1 and NIA2 impairs stomatal closure by altering genes of core ABA signaling components in Arabidopsis.
gene expression and abscissic acid signalling transduction were compared in wild-type Columbia-0 (Col-0) and the nitrate reductase mutant nia1nia2.
Title: Nitrate reductase mutation alters potassium nutrition as well as nitric oxide-mediated control of guard cell ion channels in Arabidopsis.
in response to carbon dioxide elevation, either the NR activity induction in low-nitrate plants or the NR activity inhibition in high-nitrate plants is regulated by NOS-generated nitric oxide.
Title: Elevated CO2-induced production of nitric oxide (NO) by NO synthase differentially affects nitrate reductase activity in Arabidopsis plants under different nitrate supplies.
Data suggest that regulation of NIA1 and NIA2 nitrate reductases (NR) activity by sugars does not involve HXK1 as a glucose sensor.
Title: Regulation of nitrate reduction in Arabidopsis WT and hxk1 mutant under C and N metabolites.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_106425.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables FAD binding	ISS Inferred from Sequence or Structural Similarity more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables molybdenum ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables molybdopterin cofactor binding	IEA Inferred from Electronic Annotation more info
enables nitrate reductase (NADH) activity	IEA Inferred from Electronic Annotation more info
enables nitrate reductase (NADPH) activity	IEA Inferred from Electronic Annotation more info
enables nitrate reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables nitrate reductase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within nitrate assimilation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within nitric oxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to light stimulus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in cytosol	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: nitrate reductase 1

NP_177899.1

nitrate reductase 1 (NIA1); FUNCTIONS IN: nitrate reductase activity, protein binding; INVOLVED IN: nitric oxide biosynthetic process, nitrate assimilation, response to light stimulus; LOCATED IN: cytosol; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome b5, heme-binding site (InterPro:IPR018506), Nitrate reductase NADH dependent (InterPro:IPR012137), Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Immunoglobulin E-set (InterPro:IPR014756), Cytochrome b5 (InterPro:IPR001199), NADH:cytochrome b5 reductase (CBR) (InterPro:IPR001834), Oxidoreductase, molybdopterin-binding domain (InterPro:IPR000572), Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Oxidoreductase, molybdopterin binding site (InterPro:IPR022407), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Eukaryotic molybdopterin oxidoreductase (InterPro:IPR008335), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709), Moybdenum cofactor oxidoreductase, dimerisation (InterPro:IPR005066); BEST Arabidopsis thaliana protein match is: nitrate reductase 2 (TAIR:AT1G37130.1); Has 14813 Blast hits to 14437 proteins in 2198 species: Archae - 168; Bacteria - 6976; Metazoa - 1728; Fungi - 2215; Plants - 1479; Viruses - 3; Other Eukaryotes - 2244 (source: NCBI BLink).