U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    ENGase85B Glycosyl hydrolase family 85 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 820275, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    ENGase85B
    Official Full Name
    Glycosyl hydrolase family 85
    Primary source
    TAIR:AT3G11040
    Locus tag
    AT3G11040
    See related
    Araport:AT3G11040
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    AtENGase85B; Endo-beta-N-acetyglucosaminidase 85B
    Summary
    Encodes a cytosolic beta-endo-N-acetyglucosaminidase (ENGase). ENGases N-glycans cleave the O-glycosidic linkage between the two GlcNAc residues of the N-glycan core structure and thus generate a protein with a single GlcNAc attached to asparagine.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See ENGase85B in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    11
    Sequence:
    Chromosome: 3; NC_003074.8 (3459939..3463484)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene DCD (Development and Cell Death) domain protein Neighboring gene receptor like protein 34 Neighboring gene DRE/CRT-binding protein 2B Neighboring gene TRICHOME BIREFRINGENCE-LIKE 32 Neighboring gene ferritin 2 Neighboring gene uncharacterized protein Neighboring gene Outer membrane OMP85 family protein

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. The two endo-β-N-acetylglucosaminidase genes from Arabidopsis thaliana encode cytoplasmic enzymes controlling free N-glycan levels. Fischl RM, et al. Plant Mol Biol, 2011 Oct. PMID 21796445
    2. Double-knockout of putative endo-β-N-acetylglucosaminidase (ENGase) genes in Arabidopsis thaliana: loss of ENGase activity induced accumulation of high-mannose type free N-glycans bearing N,N'-acetylchitobiosyl unit. Kimura Y, et al. Biosci Biotechnol Biochem, 2011. PMID 21597164
    3. Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana. Salanoubat M, et al. Nature, 2000 Dec 14. PMID 11130713

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data indicated that At5g05460 and At3g11040 were referred to as AtENGase85A and AtENGase85B respectively, reflecting their classification as Cazy family number 85 enzymes.
      Title: The two endo-β-N-acetylglucosaminidase genes from Arabidopsis thaliana encode cytoplasmic enzymes controlling free N-glycan levels.
    Submit: New GeneRIF Correction

    General gene information

    Go to the top of the page Help

    Markers

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within N-glycan processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within protein deglycosylation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    Glycosyl hydrolase family 85
    NP_001319520.1
    • Glycosyl hydrolase family 85; FUNCTIONS IN: hydrolase activity, acting on glycosyl bonds, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity; INVOLVED IN: biological_process unknown; LOCATED IN: cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 85 (InterPro:IPR005201), Glycoside hydrolase, catalytic core (InterPro:IPR017853); BEST Arabidopsis thaliana protein match is: Glycosyl hydrolase family 85 (TAIR:AT5G05460.1); Has 486 Blast hits to 477 proteins in 213 species: Archae - 0; Bacteria - 256; Metazoa - 108; Fungi - 38; Plants - 49; Viruses - 0; Other Eukaryotes - 35 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      3459939..3463484
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001337912.1 → NP_001327589.1  Glycosyl hydrolase family 85 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1I9LSS7, A0A654F600

    2. NM_111941.2 → NP_187715.2  Glycosyl hydrolase family 85 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1I9LSS8, A0A654F600

    3. NM_001337911.1 → NP_001319520.1  Glycosyl hydrolase family 85 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9SRL4
      UniProtKB/TrEMBL
      A0A2H1ZEI4
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.