NADP-ME2 NADP-malic enzyme 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: NADP-ME2
Official Full Name: NADP-malic enzyme 2
Primary source: TAIR:AT5G11670
Locus tag: AT5G11670
See related: Araport:AT5G11670
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: Arabidopsis thaliana NADP-malic enzyme 2; ATNADP-ME2; NADP-malic enzyme 2; T22P22.60; T22P22_60
Summary: The malic enzyme (EC 1.1.1.40) encoded by AtNADP-ME2 is presumably a cytosolic enzyme involved in malate metabolism and possibly assisting the oxidative pentose phosphate pathway. AtNADP-ME2 counts for the major part of NADP-ME activity in mature tissues of Arabidopsis.
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Genomic context

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See NADP-ME2 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 19

Sequence:: Chromosome: 5; NC_003076.8 (3754091..3758342)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural insights into the allosteric site of Arabidopsis NADP-malic enzyme 2: role of the second sphere residues in the regulatory signal transmission.
Title: Structural insights into the allosteric site of Arabidopsis NADP-malic enzyme 2: role of the second sphere residues in the regulatory signal transmission.
Arabidopsis plants over-expressing NADP-ME2 exhibited reduced rosette and root sizes, delayed flowering time and increased sensitivity to mannitol and polyethylene glycol; they also presented a higher content of C4 organic acids and sugars.
Title: Enhanced cytosolic NADP-ME2 activity in A. thaliana affects plant development, stress tolerance and specific diurnal and nocturnal cellular processes.
NADP-ME2 transcripts accumulated in response to ozone or in mutants undergoing intracellular oxidative stress. Loss of NADP-ME2 function had little effect on cat2-triggered changes in glutathione or NADPH.
Title: Analysis of knockout mutants suggests that Arabidopsis NADP-MALIC ENZYME2 does not play an essential role in responses to oxidative stress of intracellular or extracellular origin.
NADP-ME2 is an important player in plant basal defence, where it appears to be involved in the generation of reactive oxygen species
Title: Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum.
although the active sites of NADP-ME2 and NADP-ME3 are highly conserved, both isoenzymes acquire different allosteric sites, leading to the creation of proteins with unique regulatory mechanisms
Title: Identification of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_121205.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables NAD binding	IEA Inferred from Electronic Annotation more info
enables malate dehydrogenase (decarboxylating) (NADP+) activity	IDA Inferred from Direct Assay more info	PubMed
enables oxaloacetate decarboxylase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within malate metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within malate metabolic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within pentose-phosphate shunt, oxidative branch	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within response to cadmium ion	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in apoplast	HDA more info	PubMed
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in cytosol	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in plant-type cell wall	HDA more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed
located_in plasma membrane	HDA more info	PubMed
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: NADP-malic enzyme 2

NP_196728.1

NADP-malic enzyme 2 (NADP-ME2); FUNCTIONS IN: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity, oxidoreductase activity, acting on NADH or NADPH, NAD or NADP as acceptor, malic enzyme activity; INVOLVED IN: response to cadmium ion, malate metabolic process, pentose-phosphate shunt, oxidative branch, protein homooligomerization; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NADP-malic enzyme 3 (TAIR:AT5G25880.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).