CYT1 Glucose-1-phosphate adenylyltransferase family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: CYT1
Official Full Name: Glucose-1-phosphate adenylyltransferase family protein
Primary source: TAIR:AT2G39770
Locus tag: AT2G39770
See related: Araport:AT2G39770
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: CYTOKINESIS DEFECTIVE 1; EMB101; EMBRYO DEFECTIVE 101; GDP-MANNOSE PYROPHOSPHORYLASE 1; GMP1; SENSITIVE TO OZONE 1; SOZ1; T5I7.7; T5I7_7; VITAMIN C DEFECTIVE 1; VTC1
Summary: Encodes a GDP-mannose pyrophosphorylase/ mannose-1-pyrophosphatase. This enzyme provides GDP-mannose, which is used for cell wall carbohydrate biosynthesis and protein glycosylation as well as for ascorbate (vitamin C) biosynthesis. Mutations in this gene confer hypersensitivity to NH4+.
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Genomic context

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See CYT1 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 7

Sequence:: Chromosome: 2; NC_003071.7 (16588556..16591032)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1 have been described.
Title: Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1.
Data reveal that the 27th amino acid of VTC1 confers a key regulation in the interaction with CSN5B and AsA biosynthesis, as well as in Arabidopsis seedling growth.
Title: D27E mutation of VTC1 impairs the interaction with CSN5B and enhances ascorbic acid biosynthesis and seedling growth in Arabidopsis.
VTC1 is degraded in the dark through the 26S proteasome pathway, and this degradation requires CSN5B.
Title: Arabidopsis CSN5B interacts with VTC1 and modulates ascorbic acid synthesis.
Arabidopsis vtc1 and vtc2 mutants, that have low levels of ascorbate, were more susceptible to Alternaria brassicicola.
Title: Metabolite profiling of Arabidopsis inoculated with Alternaria brassicicola reveals that ascorbate reduces disease severity.
A mutation in vtc1 causes hypersensitivity to ammonium, resulting in root growth inhibition, altered ammonium metabolism, and hormone homeostasis.
Title: A mutation in GDP-mannose pyrophosphorylase causes conditional hypersensitivity to ammonium, resulting in Arabidopsis root growth inhibition, altered ammonium metabolism, and hormone homeostasis.
Programmed cell death symptoms such as nuclear chromatin condensation, the presence of multivesicular bodies, and extensive degradation and disorganization of the grana stacks were observed in 10-week-old vtc1 leaves.
Title: Modulation of plant morphology, root architecture, and cell structure by low vitamin C in Arabidopsis thaliana.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001202784.1

Supported by ESTs:

NM_001336797.1

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables mannose-1-phosphate guanylyltransferase (GTP) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables mannose-1-phosphate guanylyltransferase (GTP) activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in GDP-mannose biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within L-ascorbic acid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellulose biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to ammonium ion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to jasmonic acid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to ozone	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to salt stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Glucose-1-phosphate adenylyltransferase family protein

NP_001189713.1

CYTOKINESIS DEFECTIVE 1 (CYT1); CONTAINS InterPro DOMAIN/s: Trimeric LpxA-like (InterPro:IPR011004), Bacterial transferase hexapeptide repeat (InterPro:IPR001451), Nucleotidyl transferase (InterPro:IPR005835); BEST Arabidopsis thaliana protein match is: Glucose-1-phosphate adenylyltransferase family protein (TAIR:AT3G55590.1).

NP_181507.1

CYTOKINESIS DEFECTIVE 1 (CYT1); CONTAINS InterPro DOMAIN/s: Trimeric LpxA-like (InterPro:IPR011004), Bacterial transferase hexapeptide repeat (InterPro:IPR001451), Nucleotidyl transferase (InterPro:IPR005835); BEST Arabidopsis thaliana protein match is: Glucose-1-phosphate adenylyltransferase family protein (TAIR:AT3G55590.1); Has 29262 Blast hits to 29252 proteins in 3030 species: Archae - 1046; Bacteria - 20499; Metazoa - 437; Fungi - 382; Plants - 510; Viruses - 0; Other Eukaryotes - 6388 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

16588556..16591032

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_129535.4 → NP_181507.1 Glucose-1-phosphate adenylyltransferase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_181507.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O22287, Q9C5B8

UniProtKB/TrEMBL

A0A178VW16, A0A5S9X5H6

Conserved Domains (2) summary

cd06425
Location:1 → 234

M1P_guanylylT_B_like_N; N-terminal domain of the M1P-guanylyltransferase B-isoform like proteins

cl00160
Location:256 → 335

LbetaH; Left-handed parallel beta-Helix (LbetaH or LbH) domain: The alignment contains 5 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X). Proteins containing hexapeptide repeats are often enzymes ...
NM_001202784.1 → NP_001189713.1 Glucose-1-phosphate adenylyltransferase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001189713.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O22287, Q9C5B8

UniProtKB/TrEMBL

A0A178VW16, A0A5S9X5H6

Conserved Domains (2) summary

cd06425
Location:1 → 234

M1P_guanylylT_B_like_N; N-terminal domain of the M1P-guanylyltransferase B-isoform like proteins

cl00160
Location:256 → 335

LbetaH; Left-handed parallel beta-Helix (LbetaH or LbH) domain: The alignment contains 5 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X). Proteins containing hexapeptide repeats are often enzymes ...
NM_001336797.1 → NP_001325272.1 Glucose-1-phosphate adenylyltransferase family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

O22287, Q9C5B8

UniProtKB/TrEMBL

A0A178VW16, A0A5S9X5H6

Conserved Domains (2) summary

cd06425
Location:1 → 234

M1P_guanylylT_B_like_N; N-terminal domain of the M1P-guanylyltransferase B-isoform like proteins

cl00160
Location:256 → 335

LbetaH; Left-handed parallel beta-Helix (LbetaH or LbH) domain: The alignment contains 5 turns, each containing three imperfect tandem repeats of a hexapeptide repeat motif (X-[STAV]-X-[LIV]-[GAED]-X). Proteins containing hexapeptide repeats are often enzymes ...