Kcnj3 potassium inwardly-rectifying channel, subfamily J, member 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Kcnj3provided by RGD
Official Full Name: potassium inwardly-rectifying channel, subfamily J, member 3provided by RGD
Primary source: RGD:2958
See related: EnsemblRapid:ENSRNOG00000005369 AllianceGenome:RGD:2958
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables G-protein activated inward rectifier potassium channel activity; phosphatidylinositol-4,5-bisphosphate binding activity; and voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential. Contributes to inward rectifier potassium channel activity. Involved in response to electrical stimulus. Located in T-tubule and external side of plasma membrane. Orthologous to human KCNJ3 (potassium inwardly rectifying channel subfamily J member 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Heart (RPKM 234.7), Brain (RPKM 186.3) and 1 other tissue See more
Orthologs: human mouse all
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Genomic context

Location:: 3q21

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (60353990..60518110)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (39944896..40106646)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (41019898..41181070)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study is first to show direct cleavage of GIRK1 and GIRK2 subunits by caspase-3, with cleavage sites in GIRK1 (387ECLD390) and GIRK2 (349YEVD352) and suggests the possible role of caspase-3 mediated down-regulation of GIRK channel function and expression in hippocampal neuronal injury during prolonged epileptic seizures.
Title: Prolonged seizure activity causes caspase dependent cleavage and dysfunction of G-protein activated inwardly rectifying potassium channels.
Transient activation of mGluRs decreased the GIRK current evoked by GABAB and D2 receptors, although less efficaciously for D2.
Title: Calcium Release from Stores Inhibits GIRK.
Study provides evidence that Kir3.1 is involved in the pathogenesis of sinus bradycardia since its ihibition with siRNA can reverse the sinus bradycardia.
Title: Knockdown of cardiac Kir3.1 gene with siRNA can improve bradycardia in an experimental sinus bradycardia rat model.
inwardly rectifying K+ channels coupled to 5-HT1A autoreceptors display pharmacological properties generally expected for neuronal GIRK channels, but different from GIRK1-GIRK2 heteromers
Title: Pharmacological Characterization of 5-HT1A Autoreceptor-Coupled GIRK Channels in Rat Dorsal Raphe 5-HT Neurons.
Fourteen days after axotomy, GIRK1 and GIRK2 were down-regulated in DRG neurons at the mRNA and protein levels.
Title: G protein-gated inwardly rectifying potassium channel subunits 1 and 2 are down-regulated in rat dorsal root ganglion neurons and spinal cord after peripheral axotomy.
Findings point to the unique vulnerability of the PFC to the nutritional insult during early brain development, with enduring effects in adulthood on KCNJ3 expression and baseline metabolic activity.
Title: Prenatal protein malnutrition decreases KCNJ3 and 2DG activity in rat prefrontal cortex.
Basal Kir3 current in a native environment is unrelated to G-protein signaling or agonist-independent free G-beta-gamma.
Title: Differential effects of genetically-encoded Gβγ scavengers on receptor-activated and basal Kir3.1/Kir3.4 channel current in rat atrial myocytes.
Atrium-specific Kir3.x determines inducibility, dynamics, and termination of fibrillation by regulating restitution-driven alternans.
Title: Atrium-specific Kir3.x determines inducibility, dynamics, and termination of fibrillation by regulating restitution-driven alternans.
siRNA knock-down of NgR1 resulted in a selective increase of GABAB R1 and GABAB R2 protein and an increase in GIRK1.
Title: Post-transcriptional regulation of GABAB receptor and GIRK1 channels by Nogo receptor 1.
The mechanisms of regulation of GIRK by Galpha(i/o) using wild-type Galpha(i3) (Galpha(i3)WT) and Galpha(i3), were investigated.
Title: Two distinct aspects of coupling between Gα(i) protein and G protein-activated K+ channel (GIRK) revealed by fluorescently labeled Gα(i3) protein subunits.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Kcnj3 (e-PCR), detects polymorphism
- UniSTS:143660

RH142429 (e-PCR)
- UniSTS:231676

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables G-protein activated inward rectifier potassium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables G-protein activated inward rectifier potassium channel activity	IEA Inferred from Electronic Annotation more info
enables G-protein activated inward rectifier potassium channel activity	ISO Inferred from Sequence Orthology more info
enables inward rectifier potassium channel activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to inward rectifier potassium channel activity	IDA Inferred from Direct Assay more info	PubMed
contributes_to inward rectifier potassium channel activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
enables voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
enables voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in potassium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion import across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transmembrane transport	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of presynaptic membrane potential	IEA Inferred from Electronic Annotation more info
involved_in response to electrical stimulus	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
part_of I(KACh) inward rectifier potassium channel complex	ISO Inferred from Sequence Orthology more info
located_in T-tubule	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
part_of monoatomic ion channel complex	IEA Inferred from Electronic Annotation more info
is_active_in parallel fiber to Purkinje cell synapse	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
part_of voltage-gated potassium channel complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: G protein-activated inward rectifier potassium channel 1

Names: GIRK-1; GIRK1; KGA; KGB1; inward rectifier K(+) channel Kir3.1; potassium channel inwarding rectifying channel subfamily J member 3; potassium channel subunit Kir3.1 type 3 delta; potassium channel, inwardly rectifying subfamily J member 3; potassium voltage-gated channel subfamily J member 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031610.3 → NP_113798.1 G protein-activated inward rectifier potassium channel 1

See identical proteins and their annotated locations for NP_113798.1

Status: PROVISIONAL

Source sequence(s)

L25264

UniProtKB/Swiss-Prot

P63251

UniProtKB/TrEMBL

A6JF43

Related

ENSRNOP00000082842.1, ENSRNOT00000116585.2

Conserved Domains (2) summary

pfam01007
Location:47 → 187

IRK; Inward rectifier potassium channel

pfam17655
Location:194 → 364

IRK_C; Inward rectifier potassium channel C-terminal domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086021.1 Reference GRCr8

Range

60353990..60518110

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039105734.2 → XP_038961662.1 G protein-activated inward rectifier potassium channel 1 isoform X4

UniProtKB/TrEMBL

A0A8L2Q345

Related

ENSRNOP00000007335.2, ENSRNOT00000007335.6

Conserved Domains (2) summary

pfam01007
Location:47 → 187

IRK; Inward rectifier potassium channel

pfam17655
Location:194 → 234

IRK_C; Inward rectifier potassium channel C-terminal domain
XM_039105731.2 → XP_038961659.1 G protein-activated inward rectifier potassium channel 1 isoform X1

Conserved Domains (2) summary

pfam01007
Location:47 → 187

IRK; Inward rectifier potassium channel

pfam17655
Location:194 → 306

IRK_C; Inward rectifier potassium channel C-terminal domain
XM_039105732.2 → XP_038961660.1 G protein-activated inward rectifier potassium channel 1 isoform X2

UniProtKB/TrEMBL

A6JF44

Conserved Domains (2) summary

pfam01007
Location:47 → 187

IRK; Inward rectifier potassium channel

pfam17655
Location:194 → 307

IRK_C; Inward rectifier potassium channel C-terminal domain
XM_039105733.2 → XP_038961661.1 G protein-activated inward rectifier potassium channel 1 isoform X3

Conserved Domains (2) summary

pfam01007
Location:47 → 187

IRK; Inward rectifier potassium channel

pfam17655
Location:194 → 234

IRK_C; Inward rectifier potassium channel C-terminal domain