FAB1B phosphatidylinositol-4-phosphate 5-kinase family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: FAB1B
Official Full Name: phosphatidylinositol-4-phosphate 5-kinase family protein
Primary source: TAIR:AT3G14270
Locus tag: AT3G14270
See related: Araport:AT3G14270
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: FORMS APLOID AND BINUCLEATE CELLS 1B
Summary: Encodes a protein that is predicted to act as a 1-phosphatidylinositol-3-phosphate (PtdIns3P) 5-kinase based on its homology to Fab1 from yeast. It contains an FYVE domain required for binding to PtdIns3P-containing membranes in yeast, as well as a Cpn60_TCP1 homology domain plus a kinase domain. fab1a/fab1b pollen grains not viable and have defective vacuolar organization. FAB1A and FAB1B complement the enlarged vacuolar phenotype of the fission yeast ste12delta mutant.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See FAB1B in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 12

Sequence:: Chromosome: 3; NC_003074.8 (4753705..4761456)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The function of the Arabidopsis genes encoding FAB1B and FAB1D, both highly expressed in pollen, were investigated.
Title: Characterization of FAB1 phosphatidylinositol kinases in Arabidopsis pollen tube growth and fertilization.
Data indicate that single and double mutants of phosphatidylinositol 3-phosphate 5-kinases (PI3P5Ks) FAB1A, FAB1B, FAB1C and FAB1D exhibited slow stomatal closure upon ABA treatment.
Title: Rapid structural changes and acidification of guard cell vacuoles during stomatal closure require phosphatidylinositol 3,5-bisphosphate.
Results suggest that impairment of the function of Arabidopsis FAB1A/B might cause a defect in the membrane recycling capabilities of the auxin transporters and inhibit proper auxin transport into the cells in Arabidopsis.
Title: Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters.
Imbalanced expression of FAB1A/B impairs endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification.
Title: Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis.
Activity of FAB1 proteins is essential for the development of viable pollen. [FAB1B]
Title: Arabidopsis FAB1/PIKfyve proteins are essential for development of viable pollen.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

General gene information

Go to the top of the page Help

NM_001338116.1

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables 1-phosphatidylinositol-3-phosphate 5-kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables phosphatidylinositol binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within endomembrane system organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylinositol metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within pollen development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within signal transduction	ISS Inferred from Sequence or Structural Similarity more info	PubMed
acts_upstream_of_or_within stomatal closure	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vacuole organization	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: phosphatidylinositol-4-phosphate 5-kinase family protein

NP_001319548.1

FORMS APLOID AND BINUCLEATE CELLS 1B (FAB1B); CONTAINS InterPro DOMAIN/s: Chaperonin Cpn60/TCP-1 (InterPro:IPR002423), Zinc finger, FYVE-type (InterPro:IPR000306), Zinc finger, FYVE-related (InterPro:IPR017455), Zinc finger, FYVE/PHD-type (InterPro:IPR011011), Phosphatidylinositol-4-phosphate 5-kinase, core (InterPro:IPR002498); BEST Arabidopsis thaliana protein match is: 1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases (TAIR:AT4G33240.2); Has 8765 Blast hits to 8134 proteins in 458 species: Archae - 587; Bacteria - 13; Metazoa - 3836; Fungi - 1744; Plants - 978; Viruses - 3; Other Eukaryotes - 1604 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

4753705..4761456

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_112285.4 → NP_188044.1 phosphatidylinositol-4-phosphate 5-kinase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_188044.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WPY8, Q9LUM0

UniProtKB/TrEMBL

A0A5S9XC86, A0A654F727

Conserved Domains (3) summary

cd03334
Location:386 → 658

Fab1_TCP; TCP-1 like domain of the eukaryotic phosphatidylinositol 3-phosphate (PtdIns3P) 5-kinase Fab1. Fab1p is important for vacuole size regulation, presumably by modulating PtdIns(3,5)P2 effector activity. In the human homolog p235/PIKfyve deletion of this ...

cl28890
Location:35 → 102

FYVE_like_SF; FYVE domain like superfamily

cd17300
Location:1495 → 1758

PIPKc_PIKfyve; Phosphatidylinositol phosphate kinase (PIPK) catalytic domain found in 1-phosphatidylinositol-3-phosphate 5-kinase and similar proteins
NM_001338116.1 → NP_001319548.1 phosphatidylinositol-4-phosphate 5-kinase family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WPY8, Q9LUM0

UniProtKB/TrEMBL

A0A5S9XC86, A0A654F727

Conserved Domains (3) summary

cd03334
Location:386 → 658

Fab1_TCP; TCP-1 like domain of the eukaryotic phosphatidylinositol 3-phosphate (PtdIns3P) 5-kinase Fab1. Fab1p is important for vacuole size regulation, presumably by modulating PtdIns(3,5)P2 effector activity. In the human homolog p235/PIKfyve deletion of this ...

cl28890
Location:35 → 102

FYVE_like_SF; FYVE domain like superfamily

cd17300
Location:1495 → 1758

PIPKc_PIKfyve; Phosphatidylinositol phosphate kinase (PIPK) catalytic domain found in 1-phosphatidylinositol-3-phosphate 5-kinase and similar proteins