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    PREP1 presequence protease 1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 821451, updated on 18-Sep-2024

    Summary

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    Official Symbol
    PREP1
    Official Full Name
    presequence protease 1
    Primary source
    TAIR:AT3G19170
    Locus tag
    AT3G19170
    See related
    Araport:AT3G19170
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATPREP1; ATZNMP; presequence protease 1
    Summary
    Zinc metalloprotease pitrilysin subfamily A. Signal peptide degrading enzyme targeted to mitochondria and chloroplasts. Expressed only in siliques and flowers
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    Genomic context

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    See PREP1 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    20
    Sequence:
    Chromosome: 3; NC_003074.8 (6625377..6632164, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene KIP-related protein 6 Neighboring gene ATP/ADP isopentenyltransferase Neighboring gene plastid division protein Neighboring gene AP2/B3-like transcriptional factor family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Characterization of a novel zinc metalloprotease involved in degrading targeting peptides in mitochondria and chloroplasts. Moberg P, et al. Plant J, 2003 Dec. PMID 14617063
    2. Isolation and identification of a novel mitochondrial metalloprotease (PreP) that degrades targeting presequences in plants. Stahl A, et al. J Biol Chem, 2002 Nov 1. PMID 12138166
    3. Binding of divalent cations is essential for the activity of the organellar peptidasome in Arabidopsis thaliana, AtPreP. Bäckman HG, et al. FEBS Lett, 2009 Sep 3. PMID 19646442
    4. Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2. Bhushan S, et al. Plant Cell Physiol, 2005 Jun. PMID 15827031
    5. Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2. Glaser E, et al. Biol Chem, 2006 Oct-Nov. PMID 17081117

    See all (19) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. AtPrePI and AtPrePII are metalloproteases that degrade targeting peptides in mitochondria and chloroplasts. AtPrePI showed a preference for the N-terminal amphiphilic alpha-helix and positively charged amino acid residues.
      Title: Catalysis, subcellular localization, expression and evolution of the targeting peptides degrading protease, AtPreP2.
    2. The Arg/N-degron pathway targets transcription factors and regulates specific genes.
      Title: The Arg/N-degron pathway targets transcription factors and regulates specific genes.
    3. Data show that the AtPreP presequence has the capacity to target GFP into the mitochondrial matrix of all three species.
      Title: Targeting capacity and conservation of PreP homologues localization in mitochondria of different species.
    4. AtPreP functions as a peptidasome that degrades targeting peptides and other small unstructured peptides. In addition to Zn located in the catalytic site, AtPreP also contains two Mg-binding sites. [PreP1]
      Title: Binding of divalent cations is essential for the activity of the organellar peptidasome in Arabidopsis thaliana, AtPreP.
    5. The AtPreP peptidasome is required for efficient plant growth and organelle function particularly during early development.
      Title: Deletion of an organellar peptidasome PreP affects early development in Arabidopsis thaliana.
    6. AtPreP1 and AtPreP2 genes are differentially expressed in Arabidopsis
      Title: Two novel mitochondrial and chloroplastic targeting-peptide-degrading peptidasomes in A. thaliana, AtPreP1 and AtPreP2.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_001203003.1
    NM_112804.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metalloendopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within protein processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in apoplast HDA PubMed 
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast envelope HDA PubMed 
    located_in chloroplast stroma HDA PubMed 
    located_in cytosol HDA PubMed 
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    presequence protease 1
    NP_001189932.1
    • presequence protease 1 (PREP1); CONTAINS InterPro DOMAIN/s: Peptidase M16, C-terminal (InterPro:IPR007863), Peptidase M16C associated (InterPro:IPR013578), Peptidase M16, N-terminal (InterPro:IPR011765), Metalloenzyme, LuxS/M16 peptidase-like, metal-binding (InterPro:IPR011249), Peptidase M16, core (InterPro:IPR011237); BEST Arabidopsis thaliana protein match is: presequence protease 2 (TAIR:AT1G49630.2).
    NP_188548.2
    • presequence protease 1 (PREP1); FUNCTIONS IN: metalloendopeptidase activity; INVOLVED IN: response to cadmium ion, proteolysis, protein maturation by peptide bond cleavage; LOCATED IN: mitochondrion, apoplast, chloroplast stroma, chloroplast, chloroplast envelope; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Peptidase M16, C-terminal (InterPro:IPR007863), Peptidase M16C associated (InterPro:IPR013578), Peptidase M16, N-terminal (InterPro:IPR011765), Metalloenzyme, LuxS/M16 peptidase-like, metal-binding (InterPro:IPR011249), Peptidase M16, core (InterPro:IPR011237); BEST Arabidopsis thaliana protein match is: presequence protease 2 (TAIR:AT1G49630.2); Has 4222 Blast hits to 4196 proteins in 1321 species: Archae - 9; Bacteria - 2812; Metazoa - 224; Fungi - 268; Plants - 81; Viruses - 0; Other Eukaryotes - 828 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      6625377..6632164 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_112804.5 → NP_188548.2  presequence protease 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_188548.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q8RUN6, Q9LJL3
      UniProtKB/TrEMBL
      A0A178VHU1, A0A5S9XDK2, A0A654F8L9
      Conserved Domains (1) summary
      COG1026
      Location:106 → 1080
      Cym1; Zn-dependent peptidase, M16 (insulinase) family [Posttranslational modification, protein turnover, chaperones]

    2. NM_001203003.1 → NP_001189932.1  presequence protease 1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001189932.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A5S9XDK2, A0A654F8L9, F4JA10
      Conserved Domains (4) summary
      COG1026
      Location:106 → 1069
      Cym1; Zn-dependent peptidase, M16 (insulinase) family [Posttranslational modification, protein turnover, chaperones]
      pfam00675
      Location:154 → 248
      Peptidase_M16; Insulinase (Peptidase family M16)
      pfam05193
      Location:290 → 476
      Peptidase_M16_C; Peptidase M16 inactive domain
      pfam08367
      Location:557 → 801
      M16C_assoc; Peptidase M16C associated
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9LJL3.2

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