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    DRB4 double-stranded-RNA-binding protein 4 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 825455, updated on 18-Sep-2024

    Summary

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    Official Symbol
    DRB4
    Official Full Name
    double-stranded-RNA-binding protein 4
    Primary source
    TAIR:AT3G62800
    Locus tag
    AT3G62800
    See related
    Araport:AT3G62800
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATTIF3K1; double-stranded-RNA-binding protein 4
    Summary
    Encodes a nuclear dsRNA-binding protein DRB4 that interacts specifically with DCL4. May regulate DCL4 function and thereby affect miRNA biogenesis. Also has an impact on polymerase IV-dependent siRNA levels. DRB4 interacts with the P6 viral protein from Cauliflower mosaic virus and may be a target of viral silencing suppression.
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    Genomic context

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    See DRB4 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    4
    Sequence:
    Chromosome: 3; NC_003074.8 (23225526..23227512, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene Calcium-dependent lipid-binding (CaLB domain) family protein Neighboring gene NADH-ubiquinone oxidoreductase-like protein Neighboring gene complex 1 family protein / LVR family protein Neighboring gene Plant invertase/pectin methylesterase inhibitor superfamily protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway. Chiliveri SC, et al. Nucleic Acids Res, 2017 Aug 21. PMID 28575480, Free PMC Article
    2. Nuclear import of CaMV P6 is required for infection and suppression of the RNA silencing factor DRB4. Haas G, et al. EMBO J, 2008 Aug 6. PMID 18615098, Free PMC Article
    3. Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis. Pélissier T, et al. RNA, 2011 Aug. PMID 21700726, Free PMC Article
    4. The dsRNA-binding protein DRB4 interacts with the Dicer-like protein DCL4 in vivo and functions in the trans-acting siRNA pathway. Nakazawa Y, et al. Plant Mol Biol, 2007 Apr. PMID 17221360
    5. Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4. Fukudome A, et al. RNA, 2011 Apr. PMID 21270136, Free PMC Article

    See all (17) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. High DRB4 expression is associated with defense against turnip crinkle virus.
      Title: COP1, a negative regulator of photomorphogenesis, positively regulates plant disease resistance via double-stranded RNA binding proteins.
    2. Data indicate a potential role of the C-terminal region of double-stranded-RNA-binding protein 4 (DRB4) in protein:protein interaction as it possesses six PxxP motifs, binds to Zn2+ and contains a small structural domain.
      Title: DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway.
    3. The DRB7.1-DRB4 complex can antagonize RNase III activity and the production of siRNAs in plants.
      Title: A complex of Arabidopsis DRB proteins can impair dsRNA processing.
    4. Turnip crinkle virus infection promotes the cytosolic accumulation of the otherwise primarily nuclear DRB4, and this in turn inhibits HRT-DRB4 interaction.
      Title: RNA silencing components mediate resistance signaling against turnip crinkle virus.
    5. DCL4 and its interacting partner dsRNA-binding protein 4 (DRB4) participate in the antiviral response to Turnip yellow mosaic virus (TYMV), and that both proteins are required for TYMV-derived small RNA production.
      Title: Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection.
    6. APC10 interacts with DRB4 through the second dsRNA binding motif (dsRBD2) of DRB4, which is also required for its homodimerization and binding to its Dicer partner DCL4.
      Title: APC/C-mediated degradation of dsRNA-binding protein 4 (DRB4) involved in RNA silencing.
    7. show that the double-stranded RNA binding proteins DRB2 and DRB4 are required for proper accumulation of p4-siRNAs.
      Title: Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis.
    8. DRB4 is specifically required for DCL4 activity in vitro. dsRNA-binding domains are critical for DRB4 function.
      Title: Specific requirement of DRB4, a dsRNA-binding protein, for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4.
    9. a fraction of P6 immunoprecipitates with DRB4 in CaMV-infected cells (viral P6).
      Title: Nuclear import of CaMV P6 is required for infection and suppression of the RNA silencing factor DRB4.
    10. implicate DRB4 in antiviral RNA silencing
      Title: Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_001161214.2
    NM_116145.4
    NM_202751.1

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables double-stranded RNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNAi-mediated antiviral immune response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within defense response to virus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in defense response to virus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within miRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within ta-siRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm ISM
    Inferred from Sequence Model
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    double-stranded-RNA-binding protein 4
    NP_001154686.1
    • double-stranded-RNA-binding protein 4; CONTAINS InterPro DOMAIN/s: Double-stranded RNA-binding (InterPro:IPR001159), Double-stranded RNA-binding-like (InterPro:IPR014720); BEST Arabidopsis thaliana protein match is: dsRNA-binding protein 3 (TAIR:AT3G26932.2); Has 937 Blast hits to 871 proteins in 285 species: Archae - 0; Bacteria - 437; Metazoa - 161; Fungi - 4; Plants - 271; Viruses - 0; Other Eukaryotes - 64 (source: NCBI BLink).
    NP_191839.2
    • double-stranded-RNA-binding protein 4 (DRB4); FUNCTIONS IN: double-stranded RNA binding, protein binding; INVOLVED IN: production of ta-siRNAs involved in RNA interference; LOCATED IN: nucleus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Double-stranded RNA-binding (InterPro:IPR001159), Double-stranded RNA-binding-like (InterPro:IPR014720); BEST Arabidopsis thaliana protein match is: dsRNA-binding protein 3 (TAIR:AT3G26932.2); Has 942 Blast hits to 869 proteins in 284 species: Archae - 0; Bacteria - 434; Metazoa - 166; Fungi - 2; Plants - 266; Viruses - 0; Other Eukaryotes - 74 (source: NCBI BLink).
    NP_974480.1
    • double-stranded-RNA-binding protein 4 (DRB4); FUNCTIONS IN: double-stranded RNA binding, protein binding; INVOLVED IN: production of ta-siRNAs involved in RNA interference; LOCATED IN: nucleus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Double-stranded RNA-binding (InterPro:IPR001159), Double-stranded RNA-binding-like (InterPro:IPR014720); BEST Arabidopsis thaliana protein match is: dsRNA-binding protein 3 (TAIR:AT3G26932.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      23225526..23227512 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_116145.4 → NP_191839.2  double-stranded-RNA-binding protein 4 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_191839.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B5BUY7, Q8H1D4, Q8VXY2, Q9LZI5
      UniProtKB/TrEMBL
      A0A7G2EX21
      Conserved Domains (1) summary
      cd00048
      Location:83 → 148
      DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...

    2. NM_001161214.2 → NP_001154686.1  double-stranded-RNA-binding protein 4 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001154686.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A178VJ40, A0A7G2EX21
      Conserved Domains (1) summary
      cd00048
      Location:83 → 148
      DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...

    3. NM_202751.1 → NP_974480.1  double-stranded-RNA-binding protein 4 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_974480.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      B5BUY7, Q8H1D4, Q8VXY2, Q9LZI5
      UniProtKB/TrEMBL
      A0A7G2EX21
      Conserved Domains (1) summary
      cd00048
      Location:83 → 148
      DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8H1D4.1

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