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    HEN1 double-stranded RNA binding protein-related / DsRBD protein-like protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 827839, updated on 18-Sep-2024

    Summary

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    Official Symbol
    HEN1
    Official Full Name
    double-stranded RNA binding protein-related / DsRBD protein-like protein
    Primary source
    TAIR:AT4G20910
    Locus tag
    AT4G20910
    See related
    Araport:AT4G20910
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    CORYMBOSA 2; CRM2; HUA ENHANCER 1; T13K14.70; T13K14_70
    Summary
    Encodes an enhancer of hua1 and hua2 tha acts to specify reproductive organ identities and to repress A gene function. HEN1 also shares AG's non-homeotic function in controlling floral determinacy. Mutants display corymb-like inflorescences. HEN1 is a methyltransferase that methylates miRNAs and siRNAs on the ribose of the last nucleotide. The 3'-end methylation probably protects the 3' ends of the small RNAs from uridylation.
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    Genomic context

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    See HEN1 in Genome Data Viewer
    Location:
    chromosome: 4
    Exon count:
    10
    Sequence:
    Chromosome: 4; NC_003075.7 (11185936..11191123, complement)

    Chromosome 4 - NC_003075.7Genomic Context describing neighboring genes Neighboring gene ethylene-responsive nuclear protein / ethylene-regulated nuclear protein (ERT2) Neighboring gene tubulin beta-9 chain Neighboring gene Tetratricopeptide repeat (TPR)-like superfamily protein Neighboring gene double-stranded RNA-binding domain (DsRBD)-containing protein Neighboring gene 6-phosphogluconate dehydrogenase family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003075.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. HEN1 functions pleiotropically in Arabidopsis development and acts in C function in the flower. Chen X, et al. Development, 2002 Mar. PMID 11874905, Free PMC Article
    2. Formation of corymb-like inflorescences due to delay in bolting and flower development in the corymbosa2 mutant of Arabidopsis. Suzuki M, et al. Plant Cell Physiol, 2002 Mar. PMID 11917084
    3. Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance. Boutet S, et al. Curr Biol, 2003 May 13. PMID 12747833, Free PMC Article
    4. Mechanistic insights into small RNA recognition and modification by the HEN1 methyltransferase. Plotnikova A, et al. Biochem J, 2013 Jul 15. PMID 23621770
    5. Methylation as a crucial step in plant microRNA biogenesis. Yu B, et al. Science, 2005 Feb 11. PMID 15705854, Free PMC Article

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Investigating the Viral Suppressor HC-Pro Inhibiting Small RNA Methylation through Functional Comparison of HEN1 in Angiosperm and Bryophyte.
      Title: Investigating the Viral Suppressor HC-Pro Inhibiting Small RNA Methylation through Functional Comparison of HEN1 in Angiosperm and Bryophyte.
    2. De Novo Shoot Regeneration Controlled by HEN1 and TCP3/4 in Arabidopsis.
      Title: De Novo Shoot Regeneration Controlled by HEN1 and TCP3/4 in Arabidopsis.
    3. we show HESO1 may also act redundantly with URT1 in miRNA uridylation when HEN1 is fully competent
      Title: Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis.
    4. Light-activated HEN1 expression depended on the photoreceptors phytochrome A (phyA), phyB, cryptochrome 1 (cry1), and cry2 and key transcriptional regulators ELONGATED HYPOCOTYL5 (HY5) and HY5-HOMOLOG.
      Title: HUA ENHANCER1 is involved in posttranscriptional regulation of positive and negative regulators in Arabidopsis photomorphogenesis.
    5. Data suggest a mechanism of plant miRNA maturation which involves binding of the HEN1 methyltransferase to the DICER-LIKE 1 ribonuclease DCL1*RNA-binding protein HYL1*miRNA complex.
      Title: Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins.
    6. Data indicate that the ago1 mutation in hen1 suppressed micriRNA 3' modifications.
      Title: Plant microRNAs display differential 3' truncation and tailing modifications that are ARGONAUTE1 dependent and conserved across species.
    7. AGO1, HYL1, and HEN1 function in proximal-distal and vascular patterning of leaves.
      Title: The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis.
    8. AGO2 and HEN1 participate in the DCL2-mediated antiviral defense to ensure the survival of Turnip crinkle virus-infected plants at high temperature.
      Title: Temperature-dependent survival of Turnip crinkle virus-infected arabidopsis plants relies on an RNA silencing-based defense that requires dcl2, AGO2, and HEN1.
    9. Motif mapping analysis revealed that the amino acids located between positions 139 and 320 of Zucchini yellow mosaic virus HC-Pro were associated with Arabidopsis HEN1 interaction.
      Title: The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro.
    10. A mutation in RNA-dependent RNA polymerase 2 rescued the defects in miRNA methylation of hen1-2, revealing a previously unsuspected, negative influence of siRNAs on HEN1-mediated miRNA methylation.
      Title: siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    NM_001203853.2
    NM_118209.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables RNA methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA methyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables peptidyl-prolyl cis-trans isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within RNAi-mediated antiviral immune response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within leaf proximal/distal pattern formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within leaf vascular tissue pattern formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within miRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within miRNA processing NAS
    Non-traceable Author Statement
    more info
    		  
    acts_upstream_of_or_within miRNA-mediated gene silencing by mRNA destabilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of flower development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within siRNA processing IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within specification of floral organ identity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm ISM
    Inferred from Sequence Model
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    double-stranded RNA binding protein-related / DsRBD protein-like protein
    NP_001190782.1
    • HUA ENHANCER 1 (HEN1); FUNCTIONS IN: RNA methyltransferase activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus, cytoplasm; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA-binding protein Lupus La (InterPro:IPR006630), Methyltransferase type 12 (InterPro:IPR013217); BEST Arabidopsis thaliana protein match is: double-stranded RNA-binding domain (DsRBD)-containing protein (TAIR:AT4G20920.1).
    NP_567616.1
    • HUA ENHANCER 1 (HEN1); FUNCTIONS IN: RNA methyltransferase activity; INVOLVED IN: in 6 processes; LOCATED IN: nucleus, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA-binding protein Lupus La (InterPro:IPR006630), Methyltransferase type 12 (InterPro:IPR013217); BEST Arabidopsis thaliana protein match is: double-stranded RNA-binding domain (DsRBD)-containing protein (TAIR:AT4G20920.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003075.7 Reference assembly

      Range
      11185936..11191123 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001203853.2 → NP_001190782.1  double-stranded RNA binding protein-related / DsRBD protein-like protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001190782.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O65309, Q945R3, Q9C5Q8, Q9SUC2
      UniProtKB/TrEMBL
      A0A178UWW8
      Conserved Domains (1) summary
      pfam12847
      Location:715 → 827
      Methyltransf_18; Methyltransferase domain

    2. NM_118209.3 → NP_567616.1  double-stranded RNA binding protein-related / DsRBD protein-like protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_567616.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O65309, Q945R3, Q9C5Q8, Q9SUC2
      UniProtKB/TrEMBL
      A0A178UWW8
      Conserved Domains (1) summary
      pfam12847
      Location:715 → 827
      Methyltransf_18; Methyltransferase domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9C5Q8.1

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