U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    THI1 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 835567, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    THI1
    Official Full Name
    thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
    Primary source
    TAIR:AT5G54770
    Locus tag
    AT5G54770
    See related
    Araport:AT5G54770
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    MBG8.3; MBG8_3; THI1 PROTEIN; THI4; THIAMINE4; THIAZOLE REQUIRING; TZ
    Summary
    Encodes a thiamine biosynthetic gene that has a dual function in thiamine biosynthesis and mitochondrial DNA damage tolerance. It appears to be involved in producing the thiazole portion of thiamine (vitamin B1). A crystal structure of the protein reveals that it forms a 2-ring homo-octamer.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See THI1 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    3
    Sequence:
    Chromosome: 5; NC_003076.8 (22246519..22248332)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene Transport protein particle (TRAPP) component Neighboring gene Translation initiation factor SUI1 family protein Neighboring gene Ypt/Rab-GAP domain of gyp1p superfamily protein Neighboring gene ncRNA Neighboring gene ncRNA

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s. Joshi J, et al. Biochem J, 2020 Jun 12. PMID 32441748
    2. Thi1, a thiamine biosynthetic gene in Arabidopsis thaliana, complements bacterial defects in DNA repair. Machado CR, et al. Plant Mol Biol, 1996 Jun. PMID 8790291
    3. Dual targeting properties of the N-terminal signal sequence of Arabidopsis thaliana THI1 protein to mitochondria and chloroplasts. Chabregas SM, et al. Plant Mol Biol, 2001 Aug. PMID 11575719
    4. Point mutation is responsible for Arabidopsis tz-201 mutant phenotype affecting thiamin biosynthesis. Papini-Terzi FS, et al. Plant Cell Physiol, 2003 Aug. PMID 12941878
    5. Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana. Godoi PH, et al. J Biol Chem, 2006 Oct 13. PMID 16912043

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
      Title: Metabolic engineering provides insight into the regulation of thiamin biosynthesis in plants.
    2. Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s.
      Title: Bioinformatic and experimental evidence for suicidal and catalytic plant THI4s.
    3. Consistent with their contrasting regulatory role over stomatal closure, THI1 suppressed CPK33 kinase activity in vitro.
      Title: THI1, a Thiamine Thiazole Synthase, Interacts with Ca2+-Dependent Protein Kinase CPK33 and Modulates the S-Type Anion Channels and Stomatal Closure in Arabidopsis.
    4. The THI1 alanine to valine substitution directly affects the rate of the thiazole biosynthesis.
      Title: THI1, a protein involved in the biosynthesis of thiamin in Arabidopsis thaliana: structural analysis of THI1(A140V) mutant.
    5. Data indicate that the THI4 (AT5G54770) and the ThiM (AT3G24030)/THI4 mutants did not grow, as was expected, and the ThiM single mutant grew normally.
      Title: Identification of the thiamin salvage enzyme thiazole kinase in Arabidopsis and maize.
    6. THI1 is targeted to both chloroplasts and mitochondria and is expressed in both shoots and roots during vegetative growth but more active in shoot tissues of mature plants. Expression is affected by high salinity, sugar deprivation and flooding.
      Title: Functional characterization of the thi1 promoter region from Arabidopsis thaliana.
    7. A point mutation in the thi1 gene, involved in the synthesis of thiamin is responsible for the tz phenotype where the cotyledon is green but the leaves are white, and the plants die early during development.
      Title: Point mutation is responsible for Arabidopsis tz-201 mutant phenotype affecting thiamin biosynthesis.
    8. analysis of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana
      Title: Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana.
    9. Localization to endosomes.
      Title: Functional differentiation of endosomes in Arabidopsis cells.
    Submit: New GeneRIF Correction

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help
    NM_124858.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding HDA PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within oxazole or thiazole biosynthetic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within oxazole or thiazole biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		  
    acts_upstream_of_or_within response to cold IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within thiamine biosynthetic process IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within thiamine biosynthetic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  
    located_in chloroplast envelope HDA PubMed 
    located_in chloroplast stroma HDA PubMed 
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plastid HDA PubMed 
    located_in stromule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in thylakoid HDA PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)
    NP_200288.1
    • THI1; FUNCTIONS IN: protein homodimerization activity, zinc ion binding; INVOLVED IN: oxazole or thiazole biosynthetic process, response to cold, thiamin biosynthetic process, response to DNA damage stimulus; LOCATED IN: in 6 components; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine biosynthesis Thi4 protein (InterPro:IPR002922); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      22246519..22248332
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_124858.4 → NP_200288.1  thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_200288.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q38814
      UniProtKB/TrEMBL
      A0A178UNL2, A0A5S9YEA7
      Conserved Domains (1) summary
      PLN02661
      Location:2 → 349
      PLN02661; Putative thiazole synthesis
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q38814.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Other Literature Sources
    Molecular Biology Databases
    Research Materials
    Tools