CDPK6 calcium-dependent protein kinase 6 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: CDPK6
Official Full Name: calcium-dependent protein kinase 6
Primary source: TAIR:AT4G23650
Locus tag: AT4G23650
See related: Araport:AT4G23650
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtCDPK6; Calcium dependent protein kinase 3; calcium-dependent protein kinase 6; CALCIUM-DEPENDENT PROTEIN KINASE 6; CPK3; F9D16.120; F9D16_120
Summary: Encodes calcium dependent protein kinase 3 (CPK3), a member of the Arabidopsis CDPK gene family. CDPKs contain an intrinsic Ca2+-activation domain with four EF hand Ca2+-binding sites. CDPKs protein kinases have been proposed to function in multiple plant signal transduction pathways downstream of [Ca2+]cyt elevations, thus transducing various physiological responses. CPK3 is expressed in both guard cells and mesophyll cells. Functions in guard cell ion channel regulation. ABA and Ca(2+) activation of slow-type anion channels and, interestingly, ABA activation of plasma membrane Ca(2+)-permeable channels were impaired in independent alleles of single and double cpk3cpk6 mutant guard cells. Furthermore, ABA- and Ca(2+)-induced stomatal closing were partially impaired in these cpk3cpk6 mutant alleles. CPK6 is also a member of the Arabidopsis CDPK family.
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Genomic context

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See CDPK6 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 9

Sequence:: Chromosome: 4; NC_003075.7 (12324758..12327651, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity.
Title: Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity.
Results indicate that Ca(2+)-dependent protein kinases CPK3 and CPK6 protein kinases function downstream of reactive oxygen species (ROS) production in guard cell salicylic acid (SA) signaling.
Title: Guard Cell Salicylic Acid Signaling Is Integrated into Abscisic Acid Signaling via the Ca(2+)/CPK-Dependent Pathway.
In darkness, expression of the PHR1 and UVR3 photolyase genes, responsible for photoreactivation, is maintained at a basal level through the positive action of HY5 and HYH photomorphogenesis-promoting transcription factors
Title: The Ca(2+) -dependent protein kinase CPK3 is required for MAPK-independent salt-stress acclimation in Arabidopsis.
These results demonstrate a regulatory function of Arabidopsis CDPK6 in the N-terminal phosphorylation of AtADF1.
Title: Arabidopsis CDPK6 phosphorylates ADF1 at N-terminal serine 6 predominantly.
an essential role of the vacuolar potassium channel TPK1 in salt-stress adaptation
Title: Salt stress triggers phosphorylation of the Arabidopsis vacuolar K+ channel TPK1 by calcium-dependent protein kinases (CDPKs).
Results suggest that GDI1 regulation in morphogenesis of seedlings and leaf pavement cells could be undergone through modulating the ROP signalling pathways and the phosphorylation of GDI1 by CPK3 was required for the developmental modulation.
Title: CPK3-phosphorylated RhoGDI1 is essential in the development of Arabidopsis seedlings and leaf epidermal cells.
cpk3 and cpk13 mutants showed decreased transcript levels of defense genes in S. littoralis-damaged leaves. CPK3 is also involved in a negative feedback regulation of the cytosolic Ca2+ levels after herbivory and wounding damage.
Title: Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling.
CPK3 functions in guard cell ion channel regulation and provide genetic evidence for calcium sensors that transduce stomatal ABA signaling.
Title: CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion- and Ca(2+)-permeable channels and stomatal closure.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_118496.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	HDA more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within abscisic acid-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein autophosphorylation	HDA more info	PubMed
acts_upstream_of_or_within regulation of stomatal movement	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to salt stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in cytosol	HDA more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: calcium-dependent protein kinase 6

NP_194096.1

calcium-dependent protein kinase 6 (CDPK6); FUNCTIONS IN: calmodulin-dependent protein kinase activity, protein kinase activity, kinase activity; INVOLVED IN: in 6 processes; LOCATED IN: plasma membrane, vacuole, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 17 (TAIR:AT5G12180.1); Has 137650 Blast hits to 128872 proteins in 4025 species: Archae - 155; Bacteria - 13983; Metazoa - 52031; Fungi - 17582; Plants - 29890; Viruses - 500; Other Eukaryotes - 23509 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

12324758..12327651 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_118496.4 → NP_194096.1 calcium-dependent protein kinase 6 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_194096.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q42479, Q941A2

UniProtKB/TrEMBL

A0A178V4I3, A0A5S9XVK7, A0A7G2F5G5

Conserved Domains (2) summary

PTZ00184
Location:372 → 515

PTZ00184; calmodulin; Provisional

cd05117
Location:78 → 335

STKc_CAMK; The catalytic domain of CAMK family Serine/Threonine Kinases