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    HINT4 histidine triad nucleotide-binding 4 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 827357, updated on 18-Sep-2024

    Summary

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    Official Symbol
    HINT4
    Official Full Name
    histidine triad nucleotide-binding 4
    Primary source
    TAIR:AT4G16566
    Locus tag
    AT4G16566
    See related
    Araport:AT4G16566
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    histidine triad nucleotide-binding 4
    Summary
    Encodes a protein that has an unexpected bifunctional capability in vitro. The purified enzyme has adenylylsulfate sulfohydrolase activity (E.C. 3.6.2.1) and ADP-sulfurylase activity (E.C. 2.7.7.5). The latter is activated at low pH. The enzyme can exert it phosphorylase activity on a range of related substrates in vitro, but it acts best with APS (adenosine 5'-phsophosulfate). This protein appears to function as a homodimer.
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    Genomic context

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    See HINT4 in Genome Data Viewer
    Location:
    chromosome: 4
    Exon count:
    7
    Sequence:
    Chromosome: 4; NC_003075.7 (9333620..9336072, complement)

    Chromosome 4 - NC_003075.7Genomic Context describing neighboring genes Neighboring gene HSP20-like chaperone Neighboring gene Eukaryotic aspartyl protease family protein Neighboring gene protein arginine methyltransferase 7 Neighboring gene Protein phosphatase 2C family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003075.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Dual activity of certain HIT-proteins: A. thaliana Hint4 and C. elegans DcpS act on adenosine 5'-phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP). Guranowski A, et al. FEBS Lett, 2010 Jan 4. PMID 19896942
    2. In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes. Reumann S, et al. Plant Physiol, 2009 May. PMID 19329564, Free PMC Article
    3. Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms. Reumann S, et al. Plant Cell, 2007 Oct. PMID 17951448, Free PMC Article
    4. Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling. McBride Z, et al. Mol Cell Proteomics, 2017 Nov. PMID 28887381, Free PMC Article
    5. Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana. Mayer K, et al. Nature, 1999 Dec 16. PMID 10617198

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. dual catalytic activity of Hint4 as a hydrolase and a phosphorylase was discovered.
      Title: Dual activity of certain HIT-proteins: A. thaliana Hint4 and C. elegans DcpS act on adenosine 5'-phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP).
    Submit: New GeneRIF Correction

    General gene information

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    NM_001341116.1
    NM_117757.4

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables adenylylsulfatase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sulfate adenylyltransferase (ADP) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within purine ribonucleotide metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within sulfur compound metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytosol HDA PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  
    located_in peroxisome HDA PubMed 
    located_in peroxisome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    histidine triad nucleotide-binding 4
    NP_001319963.1
    • histidine triad nucleotide-binding 4 (HINT4); FUNCTIONS IN: adenylylsulfatase activity, sulfate adenylyltransferase (ADP) activity, nucleotide binding; INVOLVED IN: sulfur metabolic process, purine ribonucleotide metabolic process, nucleotide metabolic process; LOCATED IN: peroxisome; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Histidine triad-like motif (InterPro:IPR011146), Histidine triad (HIT) protein (InterPro:IPR001310), Histidine triad motif (InterPro:IPR011151); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003075.7 Reference assembly

      Range
      9333620..9336072 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001341117.1 → NP_001329381.1  histidine triad nucleotide-binding 4 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A1P8B7J6, A0A5S9XTA6

    2. NM_001341116.1 → NP_001319963.1  histidine triad nucleotide-binding 4 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A2H1ZEM4

    3. NM_117757.4 → NP_567507.1  histidine triad nucleotide-binding 4 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_567507.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      O23500, Q84VV6, Q8L9E2
      UniProtKB/TrEMBL
      A0A178UVT4
      Conserved Domains (1) summary
      cl00228
      Location:8 → 108
      HIT_like; HIT family: HIT (Histidine triad) proteins, named for a motif related to the sequence HxHxH/Qxx (x, a hydrophobic amino acid), are a superfamily of nucleotide hydrolases and transferases, which act on the alpha-phosphate of ribonucleotides. On the basis ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q84VV6.1

    Gene LinkOut

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