asp abnormal spindle [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: aspprovided by FlyBase
Official Full Name: abnormal spindleprovided by FlyBase
Primary source: FLYBASE:FBgn0000140
Locus tag: Dmel_CG6875
See related: AllianceGenome:FB:FBgn0000140
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-96Aa; anon-WO0118547.279; Asp; ASP; CG6875; Dm Asp; Dmel\CG6875
Summary: Enables calmodulin binding activity; microtubule binding activity; and myosin light chain binding activity. Involved in several processes, including brain morphogenesis; cytoskeleton organization; and germ cell development. Located in centrosome and spindle pole. Part of microtubule associated complex. Is expressed in several structures, including CNS glial cell; cardiogenic mesoderm; central brain primordium; ganglia; and larval nervous system. Used to study microcephaly and primary autosomal recessive microcephaly 5. Human ortholog(s) of this gene implicated in microcephaly; primary autosomal recessive microcephaly; primary autosomal recessive microcephaly 5; and visual epilepsy. Orthologous to human ASPM (assembly factor for spindle microtubules). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 96A19-96A20; 3-85 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (24753849..24760513)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (20579571..20586235)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mutations in abnormal spindle disrupt temporal transcription factor expression and trigger immune responses in the Drosophila brain.
Title: Mutations in abnormal spindle disrupt temporal transcription factor expression and trigger immune responses in the Drosophila brain.
The Multiple Mitotic Roles of the ASPM Orthologous Proteins: Insight into the Etiology of ASPM-Dependent Microcephaly.
Title: The Multiple Mitotic Roles of the ASPM Orthologous Proteins: Insight into the Etiology of ASPM-Dependent Microcephaly.
Dynein, Mud and Asp operate sequentially during the cell cycle to ensure efficient centrosome-spindle coupling in mitosis, thereby preventing centrosome mis-segregation to maintain centrosome number.
Title: Sequential activities of Dynein, Mud and Asp in centrosome-spindle coupling maintain centrosome number upon mitosis.
in this study we have provided evidence that ASPM controls spindle orientation by regulating the dynamics of astral MT and that CITK is a critical downstream partner of ASPM for this activity.
Title: ASPM and CITK regulate spindle orientation by affecting the dynamics of astral microtubules.
Asp at the minus ends of the microtubules cross-links MTs at the pole and within the spindle
Title: Microcephaly protein Asp focuses the minus ends of spindle microtubules at the pole and within the spindle.
Asp-CaM complex has a critical role in centrosome-pole cohesion and centrosome inheritance in neural stem cells
Title: An Asp-CaM complex is required for centrosome-pole cohesion and centrosome inheritance in neural stem cells.
propose that Asp regulates neuroepithelium morphogenesis through myosin-II-mediated structural and mechanical processes to maintain force balance and tissue cohesiveness
Title: The microcephaly protein Asp regulates neuroepithelium morphogenesis by controlling the spatial distribution of myosin II.
asp mutation leads to severe defects in spindle microtubule organization within the germarium. The mitotic spindles of the mutant cystocytes are composed by wavy microtubules and have abnormal poles that often lack gamma-tubulin.
Title: The abnormal spindle protein is required for germ cell mitosis and oocyte differentiation during Drosophila oogenesis.
dynein, abnormal spindle protein (Asp), and KLP10A have roles in Drosophila spindle pole organization
Title: Spindle pole organization in Drosophila S2 cells by dynein, abnormal spindle protein (Asp), and KLP10A.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables calmodulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables myosin light chain binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in astral microtubule nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in brain morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome duplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cystoblast division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of meiotic spindle localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of meiotic spindle localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female germ-line stem cell asymmetric division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in kinetochore organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ovarian fusome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
part_of microtubule associated complex	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule organizing center	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: abnormal spindle

Names: CG6875-PA; abnormal spindle protein; abnormal spindles; asp-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

24753849..24760513

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079764.3 → NP_524488.3 abnormal spindle [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524488.3

Status: REVIEWED

UniProtKB/Swiss-Prot

O01401, Q8SX66, Q9VC45

UniProtKB/TrEMBL

B8A3X9, H0RNM3

Related

FBpp0084071

Conserved Domains (4) summary

smart00015
Location:1689 → 1711

IQ; Calmodulin-binding motif

cd00014
Location:866 → 968

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

pfam13868
Location:1066 → 1435

TPH; Trichohyalin-plectin-homology domain

pfam15780
Location:26 → 122

ASH; Abnormal spindle-like microcephaly-assoc'd, ASPM-SPD-2-Hydin