Gckr glucokinase regulatory protein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gckrprovided by MGI
Official Full Name: glucokinase regulatory proteinprovided by MGI
Primary source: MGI:MGI:1096345
See related: Ensembl:ENSMUSG00000059434 AllianceGenome:MGI:1096345
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GKRP
Summary: Enables kinase inhibitor activity. Acts upstream of or within intracellular glucose homeostasis; negative regulation of glucokinase activity; and protein import into nucleus. Located in nucleus. Is expressed in diencephalon lateral wall mantle layer; liver left lobe; liver right lobe; midbrain mantle layer; and retina. Human ortholog(s) of this gene implicated in end stage renal disease and type 2 diabetes mellitus. Orthologous to human GCKR (glucokinase regulator). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in liver adult (RPKM 48.2), liver E18 (RPKM 19.1) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B1; 5 17.27 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (31454594..31484646)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (31297266..31327302)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hepatic glucokinase regulatory protein and carbohydrate response element binding protein attenuation reduce de novo lipogenesis but do not mitigate intrahepatic triglyceride accumulation in Aldob deficiency.
Title: Hepatic glucokinase regulatory protein and carbohydrate response element binding protein attenuation reduce de novo lipogenesis but do not mitigate intrahepatic triglyceride accumulation in Aldob deficiency.
The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326.
Title: The GCKR-P446L gene variant predisposes to raised blood cholesterol and lower blood glucose in the P446L mouse-a model for GCKR rs1260326.
This study showed that Sirt2-dependent GKRP deacetylation improves impaired HGU and suggest that it may be a therapeutic target for type 2 diabetes.
Title: Sirt2 facilitates hepatic glucose uptake by deacetylating glucokinase regulatory protein.
Given that acetylated GKRP may affiliate with type-2 diabetes mellitus (T2DM), understanding the mechanism of GKRP acetylation in the liver could reveal novel targets within the GK-GKRP pathway, for treating T2DM and other metabolic pathologies.
Title: Acetylation of glucokinase regulatory protein decreases glucose metabolism by suppressing glucokinase activity.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Gckr (e-PCR), detects polymorphism
- UniSTS:143188

26.mHAa8b2.seq (e-PCR)
- UniSTS:123767

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables carbohydrate binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables enzyme inhibitor activity	ISO Inferred from Sequence Orthology more info
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-6-phosphate binding	ISO Inferred from Sequence Orthology more info
enables fructose-6-phosphate binding	ISS Inferred from Sequence or Structural Similarity more info
enables glucose sensor activity	IBA Inferred from Biological aspect of Ancestor more info
enables glucose sensor activity	IEA Inferred from Electronic Annotation more info
enables glucose sensor activity	ISO Inferred from Sequence Orthology more info
enables kinase binding	IEA Inferred from Electronic Annotation more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables kinase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables kinase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in carbohydrate derivative metabolic process	IEA Inferred from Electronic Annotation more info
involved_in intracellular glucose homeostasis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within intracellular glucose homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of glucokinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of glucokinase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glucokinase activity	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein import into nucleus	ISO Inferred from Sequence Orthology more info
involved_in protein localization to nucleus	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein localization to nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to fructose	IEA Inferred from Electronic Annotation more info
involved_in response to fructose	ISO Inferred from Sequence Orthology more info
involved_in response to glucose	IEA Inferred from Electronic Annotation more info
involved_in response to glucose	ISO Inferred from Sequence Orthology more info
involved_in triglyceride homeostasis	ISO Inferred from Sequence Orthology more info
involved_in urate metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glucokinase regulatory protein

Names: glucokinase regulator

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001374741.1 → NP_001361670.1 glucokinase regulatory protein isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC114619

Consensus CDS

CCDS89900.1

UniProtKB/Swiss-Prot

A0A0J9YUI8, Q91X44

Related

ENSMUSP00000144202.2, ENSMUST00000201166.4

Conserved Domains (3) summary

PRK05441
Location:482 → 605

murQ; N-acetylmuramic acid-6-phosphate etherase; Reviewed

PRK12570
Location:30 → 277

PRK12570; N-acetylmuramic acid-6-phosphate etherase; Reviewed

cl00389
Location:293 → 555

SIS; SIS domain. SIS (Sugar ISomerase) domains are found in many phosphosugar isomerases and phosphosugar binding proteins. SIS domains are also found in proteins that regulate the expression of genes involved in synthesis of phosphosugars.
NM_144909.2 → NP_659158.1 glucokinase regulatory protein isoform 2

See identical proteins and their annotated locations for NP_659158.1

Status: VALIDATED

Source sequence(s)

AC114619

Consensus CDS

CCDS19182.1

UniProtKB/Swiss-Prot

Q91X44

Related

ENSMUSP00000072084.6, ENSMUST00000072228.9

Conserved Domains (2) summary

PRK12570
Location:30 → 277

PRK12570; N-acetylmuramic acid-6-phosphate etherase; Reviewed

cl00389
Location:293 → 366

SIS; SIS domain. SIS (Sugar ISomerase) domains are found in many phosphosugar isomerases and phosphosugar binding proteins. SIS domains are also found in proteins that regulate the expression of genes involved in synthesis of phosphosugars.

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

31454594..31484646

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006503882.5 → XP_006503945.1 glucokinase regulatory protein isoform X1

Conserved Domains (3) summary

PRK05441
Location:464 → 587

murQ; N-acetylmuramic acid-6-phosphate etherase; Reviewed

PRK12570
Location:30 → 259

PRK12570; N-acetylmuramic acid-6-phosphate etherase; Reviewed

cl00389
Location:275 → 537

SIS; SIS domain. SIS (Sugar ISomerase) domains are found in many phosphosugar isomerases and phosphosugar binding proteins. SIS domains are also found in proteins that regulate the expression of genes involved in synthesis of phosphosugars.
XM_006503883.5 → XP_006503946.1 glucokinase regulatory protein isoform X2

Conserved Domains (2) summary

PRK05441
Location:430 → 553

murQ; N-acetylmuramic acid-6-phosphate etherase; Reviewed

cl00389
Location:57 → 221

SIS; SIS domain. SIS (Sugar ISomerase) domains are found in many phosphosugar isomerases and phosphosugar binding proteins. SIS domains are also found in proteins that regulate the expression of genes involved in synthesis of phosphosugars.