mira miranda [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: miraprovided by FlyBase
Official Full Name: mirandaprovided by FlyBase
Primary source: FLYBASE:FBgn0021776
Locus tag: Dmel_CG12249
See related: AllianceGenome:FB:FBgn0021776
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG12249; Dmel\CG12249; mir; Mir; MIR; Mira; MIRA; Miranda
Summary: Enables myosin binding activity. Involved in several processes, including asymmetric protein localization involved in cell fate determination; neuroblast proliferation; and regulation of pole plasm oskar mRNA localization. Located in several cellular components, including apical cortex; basal cortex; and basal plasma membrane. Colocalizes with aster. Is expressed in several structures, including CNS glial cell; anlage in statu nascendi; head epidermis primordium; sensory nervous system primordium; and somatic precursor cell. Used to study brain cancer and cancer. [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 92B9-92B9; 3-67 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (19931799..19935120, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (15757521..15760842, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression.
Title: Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression.
Therefore, phosphorylation of Miranda by aPKC and differential binding to the actomyosin network are required at distinct phases of the cell cycle to polarize fate determinant localization in neuroblasts.
Title: aPKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts.
An interdependence of a protein (miranda) and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells.
Title: Maintenance of Miranda Localization in Drosophila Neuroblasts Involves Interaction with the Cognate mRNA.
two-step process for the basal localization of Mira during neuroblast division is revealed: cortical association of Mira mediated by the Phosphotyrosyl phosphatase activator-Protein phosphatase 4 complex followed by apical aPKC-mediated basal restriction.
Title: Phosphotyrosyl phosphatase activator facilitates localization of Miranda through dephosphorylation in dividing neuroblasts.
The role of Miranda in the localization of Staufen during the division of neuroblasts is described.
Title: The structural basis of Miranda-mediated Staufen localization during Drosophila neuroblast asymmetric division.
Miranda mislocalizes oskar mRNA/Staufen complexes to the oocyte anterior by coupling them to the bicoid localization pathway.
Title: Anterior-posterior axis specification in Drosophila oocytes: identification of novel bicoid and oskar mRNA localization factors.
aPKC phosphorylates Miranda to polarize fate determinants during neuroblast asymmetric cell division.
Title: aPKC phosphorylates Miranda to polarize fate determinants during neuroblast asymmetric cell division.
identified the evolutionarily conserved Protein Phosphatase 4 (PP4) regulatory subunit PP4R3/Falafel (Flfl) as a key mediator specific for the localization of Miranda (Mira) and associated cell fate determinants during both interphase and mitosis
Title: Protein phosphatase 4 mediates localization of the Miranda complex during Drosophila neuroblast asymmetric divisions.
Miranda protein forms an apical crescent at interphase, but is ubiquitously localized at prophase in a Myosin-II-dependent manner.
Title: Asymmetric localization of the adaptor protein Miranda in neuroblasts is achieved by diffusion and sequential interaction of Myosin II and VI.
The first solution structure of Miranda central "cargo-binding" domain (residues 460-660) using small-angle X-ray scattering was reported.
Title: Miranda cargo-binding domain forms an elongated coiled-coil homodimer in solution: implications for asymmetric cell division in Drosophila.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables myosin binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in anterior/posterior axis specification, embryo	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric neuroblast division	TAS Traceable Author Statement more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	IPI Inferred from Physical Interaction more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	TAS Traceable Author Statement more info	PubMed
involved_in ganglion mother cell fate determination	TAS Traceable Author Statement more info	PubMed
involved_in neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein localization to cell cortex	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of pole plasm oskar mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of proteolysis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral cord development	HMP more info	PubMed

Component	Evidence Code	Pubs
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in apical part of cell	TAS Traceable Author Statement more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with aster	IDA Inferred from Direct Assay more info	PubMed
located_in basal cortex	IDA Inferred from Direct Assay more info	PubMed
located_in basal cortex	TAS Traceable Author Statement more info	PubMed
located_in basal part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in basal plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	TAS Traceable Author Statement more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: miranda

Names: CG12249-PA; CG12249-PB; CG12249-PC; mira-PA; mira-PB; mira-PC

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

19931799..19935120 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057944.3 → NP_477292.1 miranda, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477292.1

Status: REVIEWED

UniProtKB/TrEMBL

O44224, Q8IN63

Related

FBpp0083257, FBtr0083848

Conserved Domains (2) summary

TIGR04211
Location:163 → 272

SH3_and_anchor; SH3 domain protein

pfam00529
Location:515 → 672

HlyD; HlyD membrane-fusion protein of T1SS
NM_001275822.1 → NP_001262751.1 miranda, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262751.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KHS3, O44224

Conserved Domains (1) summary

TIGR04211
Location:163 → 272

SH3_and_anchor; SH3 domain protein
NM_057943.4 → NP_477291.1 miranda, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477291.1

Status: REVIEWED

UniProtKB/TrEMBL

O44224, Q9VDR7

Related

FBpp0083256

Conserved Domains (1) summary

TIGR04211
Location:163 → 272

SH3_and_anchor; SH3 domain protein