Prkci protein kinase C, iota [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkciprovided by MGI
Official Full Name: protein kinase C, iotaprovided by MGI
Primary source: MGI:MGI:99260
See related: Ensembl:ENSMUSG00000037643 AllianceGenome:MGI:99260
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pkci; Pkcl; Prkcl; PKClambda; mKIAA4165; aPKClambda; 2310021H13Rik
Summary: Enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity. Involved in cellular response to insulin stimulus; positive regulation of D-glucose import; and positive regulation of protein localization to plasma membrane. Acts upstream of or within actin filament organization; establishment of apical/basal cell polarity; and eye photoreceptor cell development. Located in several cellular components, including Schmidt-Lanterman incisure; apical plasma membrane; and brush border. Is expressed in several structures, including alimentary system; brain; genitourinary system; lung; and sensory organ. Used to study abdominal obesity-metabolic syndrome; obesity; and type 2 diabetes mellitus. Orthologous to human PRKCI (protein kinase C iota). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in bladder adult (RPKM 26.7), placenta adult (RPKM 19.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 A3; 3 14.65 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (31049893..31106889)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (30995742..31052740)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Enhanced SREBP2-driven cholesterol biosynthesis by PKClambda/iota deficiency in intestinal epithelial cells promotes aggressive serrated tumorigenesis.
Title: Enhanced SREBP2-driven cholesterol biosynthesis by PKCλ/ι deficiency in intestinal epithelial cells promotes aggressive serrated tumorigenesis.
Identification of Arhgef12 and Prkci as genetic modifiers of retinal dysplasia in the Crb1rd8 mouse model.
Title: Identification of Arhgef12 and Prkci as genetic modifiers of retinal dysplasia in the Crb1rd8 mouse model.
Deficiency of PKClambda/iota alleviates the liver pathologic impairment of Schistosoma japonicum infection by thwarting Th2 response.
Title: Deficiency of PKCλ/ι alleviates the liver pathologic impairment of Schistosoma japonicum infection by thwarting Th2 response.
Atypical protein kinase C iota (PKClambda/iota) ensures mammalian development by establishing the maternal-fetal exchange interface.
Title: Atypical protein kinase C iota (PKCλ/ι) ensures mammalian development by establishing the maternal-fetal exchange interface.
LSL-Kras(G12D);Trp53(fl/fl) (KP)-mediated lung adenocarcinomas can develop through both PKCiota-dependent and PKCiota-independent pathways.
Title: Protein Kinase Cι and Wnt/β-Catenin Signaling: Alternative Pathways to Kras/Trp53-Driven Lung Adenocarcinoma.
these findings suggest that miR-29c is a negative regulator of axonal growth of dorsal root ganglia neurons by targeting PRKCI under hyperglycemia.
Title: MiR-29c/PRKCI Regulates Axonal Growth of Dorsal Root Ganglia Neurons Under Hyperglycemia.
PKClambda/iota emerges as a critical regulator of Th17 differentiation and allergic airway hyperresponsiveness.
Title: PKCλ/ι regulates Th17 differentiation and house dust mite-induced allergic airway inflammation.
This study demonstrated hat under physiological conditions, PKCiota/lambda is essential for hippocampal early-LTP and long-term memory.
Title: Learning-Induced Suboptimal Compensation for PKCι/λ Function in Mutant Mice.
the aPKC-CBP pathway is a homeostatic compensatory mechanism that modulates hippocampal neurogenesis and memory in an age-dependent manner in response to reduced CREB activity.
Title: The aPKC-CBP Pathway Regulates Adult Hippocampal Neurogenesis in an Age-Dependent Manner.
he oncogenic activity of PRKCI relates in part to the up-regulation of TNFalpha to promote an immune-suppressive tumor microenvironment characterized by an abundance of myeloid-derived suppressor cells and inhibition of cytotoxic T-cell infiltration
Title: PRKCI promotes immune suppression in ovarian cancer.

Submit: New GeneRIF Correction See all GeneRIFs (59)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (2)
Gene trapped (2)
Targeted (10) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D3Dcr2 (e-PCR), detects polymorphism
- UniSTS:507670

NoName (e-PCR)
- UniSTS:548156

Prkci (e-PCR), detects polymorphism
- UniSTS:507989

Prkci (e-PCR), detects polymorphism
- UniSTS:507990

Prkci (e-PCR), detects polymorphism
- UniSTS:507991

AI427505 (e-PCR)
- UniSTS:159103

D28577 (e-PCR)
- UniSTS:178820

Prkci (e-PCR), detects polymorphism
- UniSTS:527505

D28577 (e-PCR)
- UniSTS:124819

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in Golgi vesicle budding	IEA Inferred from Electronic Annotation more info
involved_in Golgi vesicle budding	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell migration	IEA Inferred from Electronic Annotation more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in cell-cell junction organization	ISO Inferred from Sequence Orthology more info
involved_in cell-cell junction organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to insulin stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to insulin stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of apical/basal cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of epithelial cell apical/basal polarity	IEA Inferred from Electronic Annotation more info
involved_in establishment or maintenance of epithelial cell apical/basal polarity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within eye photoreceptor cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of glial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glial cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of D-glucose import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of glial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glial cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron projection development	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	ISO Inferred from Sequence Orthology more info
involved_in response to interleukin-1	IEA Inferred from Electronic Annotation more info
involved_in response to interleukin-1	ISO Inferred from Sequence Orthology more info
involved_in response to peptide hormone	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
part_of PAR polarity complex	IEA Inferred from Electronic Annotation more info
part_of PAR polarity complex	ISO Inferred from Sequence Orthology more info
located_in Schaffer collateral - CA1 synapse	IEA Inferred from Electronic Annotation more info
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
located_in Schmidt-Lanterman incisure	IDA Inferred from Direct Assay more info	PubMed
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in bicellular tight junction	IEA Inferred from Electronic Annotation more info
located_in bicellular tight junction	ISO Inferred from Sequence Orthology more info
located_in brush border	IDA Inferred from Direct Assay more info	PubMed
located_in cell leading edge	IEA Inferred from Electronic Annotation more info
located_in cell leading edge	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in intercellular bridge	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: protein kinase C iota type

Names: aPKC-lambda/iota; atypical protein kinase C-lambda/iota; nPKC-iota; protein kinase C, lambda

NP_032883.2

EC 2.7.11.13

XP_006535474.2

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001428640.1 → NP_001415569.1 protein kinase C iota type isoform 2

Status: VALIDATED

Source sequence(s)

AC111093, AC117590

UniProtKB/TrEMBL

Q3TJJ5
NM_008857.4 → NP_032883.2 protein kinase C iota type isoform 1

See identical proteins and their annotated locations for NP_032883.2

Status: VALIDATED

Source sequence(s)

AC111093, AC117590

Consensus CDS

CCDS17289.2

UniProtKB/Swiss-Prot

Q62074

UniProtKB/TrEMBL

Q3TJJ5

Related

ENSMUSP00000103884.3, ENSMUST00000108249.9

Conserved Domains (3) summary

cd06404
Location:26 → 108

PB1_aPKC; PB1 domain is an essential modular domain of the atypical protein kinase C (aPKC) which in complex with Par6 and Par3 proteins is crucial for establishment of apical-basal polarity of animal cells. PB1 domain is a modular domain mediating specific ...

cd05618
Location:232 → 595

STKc_aPKC_iota; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota

pfam00130
Location:141 → 193

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

31049893..31106889

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006535411.5 → XP_006535474.2 protein kinase C iota type isoform X1

Conserved Domains (3) summary

cd05618
Location:202 → 565

STKc_aPKC_iota; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota

pfam00130
Location:111 → 161

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

cl02720
Location:26 → 79

PB1; The PB1 domain is a modular domain mediating specific protein-protein interactions which play a role in many critical cell processes, such as osteoclastogenesis, angiogenesis, early cardiovascular development, and cell polarity. A canonical PB1-PB1 ...