FBXO3 F-box protein 3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: FBXO3provided by HGNC
Official Full Name: F-box protein 3provided by HGNC
Primary source: HGNC:HGNC:13582
See related: Ensembl:ENSG00000110429 MIM:609089; AllianceGenome:HGNC:13582
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: FBA; FBX3
Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in thyroid (RPKM 11.3), kidney (RPKM 10.4) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 11p13

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	11	NC_000011.10 (33740944..33774520, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	11	NC_060935.1 (33877954..33911529, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	11	NC_000011.9 (33762490..33796066, complement)

Chromosome 11 - NC_000011.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

FBXO3 stabilizes USP4 and Twist1 to promote PI3K-mediated breast cancer metastasis.
Title: FBXO3 stabilizes USP4 and Twist1 to promote PI3K-mediated breast cancer metastasis.
FBXO3 potentiates vascular inflammation and atherosclerosis that can be effectively mitigated by a small molecule inhibitor.
Title: The SCFFBXO3 ubiquitin E3 ligase regulates inflammation in atherosclerosis.
reveal that FBXO46 is a crucial proteasomal regulator of FBXO31 and thereby prevents senescence in normal growth conditions
Title: The SCF(FBXO46) ubiquitin ligase complex mediates degradation of the tumor suppressor FBXO31 and thereby prevents premature cellular senescence.
we report the X-ray structure of the human FBxo3 ApaG domain, residues 278-407, at 2.0 A resolution
Title: Crystal structure and interaction studies of the human FBxo3 ApaG domain.
AIRE, which is phosphorylated on two specific residues near its N terminus, then binds to the F-box protein 3 (FBXO3) E3 ubiquitin ligase. In turn, this SCF(FBXO3) (SKP1-CUL1-F box) complex ubiquitylates AIRE, increases its binding to the positive transcription elongation factor b (P-TEFb), and potentiates its transcriptional activity.
Title: FBXO3 Protein Promotes Ubiquitylation and Transcriptional Activity of AIRE (Autoimmune Regulator).
Fbxo3 promotes the proteasomal degradation of Smurf1. Fbxo3 promotes the poly-ubiquitination of Smurf1.
Title: F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation.
Virulence factor NSs of rift valley fever virus recruits the FBXO3 to degrade subunit p62 of general transcription factor TFIIH.
Title: Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH.
controls TRAF adaptor stability to regulate inflammation
Title: A combinatorial F box protein directed pathway controls TRAF adaptor stability to regulate inflammation.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
Fbx3 formed SCF(Fbx3) ubiquitin ligase and promoted the degradation of HIPK2 and p300 by the ubiquitin-proteasome pathway
Title: PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genetics of coronary artery calcification among African Americans, a meta-analysis. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	HIV-1 Tat is identified to have a physical interaction with F-box protein 3 (FBXO3) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

FBXO3_2462 (e-PCR)
- UniSTS:280666

FBXO3 (e-PCR)
- UniSTS:504684

SHGC-44450 (e-PCR)
- UniSTS:14723

D1S3695 (e-PCR)
- UniSTS:474273

D11S4719 (e-PCR)
- UniSTS:9911

SGC44450 (e-PCR)
- UniSTS:51159

A007J20 (e-PCR)
- UniSTS:22243

WI-11454 (e-PCR)
- UniSTS:20212

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin-like ligase-substrate adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in proteolysis	TAS Traceable Author Statement more info	PubMed
involved_in response to lipopolysaccharide	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of SCF ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info

General protein information

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Preferred Names: F-box only protein 3

Names: F-box protein FBX3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012175.4 → NP_036307.2 F-box only protein 3 isoform 1

See identical proteins and their annotated locations for NP_036307.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1) which includes a highly acidic D/E-rich domain and two poly-R pieces at the C-terminus.

Source sequence(s)

BC039291, BC046110

Consensus CDS

CCDS7887.1

UniProtKB/Swiss-Prot

B3KY16, D3DR05, Q86X90, Q9H0V2, Q9NUX2, Q9UK99

UniProtKB/TrEMBL

Q59GS0

Related

ENSP00000265651.3, ENST00000265651.8

Conserved Domains (3) summary

pfam04379
Location:294 → 383

DUF525; ApaG domain

pfam09346
Location:121 → 249

SMI1_KNR4; SMI1 / KNR4 family (SUKH-1)

pfam12937
Location:13 → 57

F-box-like
NM_033406.4 → NP_208385.1 F-box only protein 3 isoform 2

See identical proteins and their annotated locations for NP_208385.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) is divergent from variant 1 at the 3' terminus. It encodes a C-terminal truncated isoform, which lacks the D/E-rich domain and poly-Rs, as compared to isoform 1.

Source sequence(s)

AC113192, AF176702, AL049629, BC017107, BC039291

Consensus CDS

CCDS44566.1

UniProtKB/TrEMBL

Q59GS0

Related

ENSP00000408836.2, ENST00000448981.6

Conserved Domains (3) summary

smart00860
Location:121 → 249

SMI1_KNR4; SMI1 / KNR4 family

pfam12937
Location:13 → 57

F-box-like; F-box-like

cl01119
Location:279 → 404

DUF525; ApaG domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000011.10 Reference GRCh38.p14 Primary Assembly

Range

33740944..33774520 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011519980.2 → XP_011518282.1 F-box only protein 3 isoform X1

Conserved Domains (3) summary

pfam04379
Location:294 → 383

DUF525; Protein of unknown function (DUF525)

pfam09346
Location:121 → 249

SMI1_KNR4; SMI1 / KNR4 family (SUKH-1)

pfam12937
Location:13 → 57

F-box-like; F-box-like
XM_047426752.1 → XP_047282708.1 F-box only protein 3 isoform X3
XM_011519981.3 → XP_011518283.1 F-box only protein 3 isoform X2

See identical proteins and their annotated locations for XP_011518283.1

UniProtKB/TrEMBL

Q59GS0

Related

ENSP00000431745.1, ENST00000534136.5

Conserved Domains (3) summary

pfam04379
Location:294 → 383

DUF525; ApaG domain

pfam09346
Location:121 → 249

SMI1_KNR4; SMI1 / KNR4 family (SUKH-1)

pfam12937
Location:13 → 57

F-box-like