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    rock2b rho-associated, coiled-coil containing protein kinase 2b [ Danio rerio (zebrafish) ]

    Gene ID: 321070, updated on 9-Dec-2024

    Summary

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    Official Symbol
    rock2bprovided by ZNC
    Official Full Name
    rho-associated, coiled-coil containing protein kinase 2bprovided by ZNC
    Primary source
    ZFIN:ZDB-GENE-060125-2
    See related
    Ensembl:ENSDARG00000004877 AllianceGenome:ZFIN:ZDB-GENE-060125-2
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Danio rerio
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
    Also known as
    cb64; sb:cb64; si:dkey-65b13.7; si:dkey-65b13.10
    Summary
    Predicted to enable Rho-dependent protein serine/threonine kinase activity. Acts upstream of or within Kupffer's vesicle development; epithelial cilium movement involved in determination of left/right asymmetry; and heart morphogenesis. Located in extracellular region. Is expressed in several structures, including EVL; Kupffer's vesicle; forerunner cell group; hindbrain; and somite. Orthologous to human ROCK2 (Rho associated coiled-coil containing protein kinase 2). [provided by Alliance of Genome Resources, Dec 2024]
    Orthologs
    all
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    Genomic context

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    See rock2b in Genome Data Viewer
    Location:
    chromosome: 20
    Exon count:
    45
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCz11 (GCF_000002035.6) 20 NC_007131.7 (33575452..33705224, complement)
    105 previous assembly GRCz10 (GCF_000002035.5) 20 NC_007131.6 (33672573..33804146, complement)

    Chromosome 20 - NC_007131.7Genomic Context describing neighboring genes Neighboring gene rho-associated protein kinase 2-like Neighboring gene si:dkey-65b13.8 Neighboring gene rho-associated protein kinase 2-like Neighboring gene golgin subfamily A member 6-like protein 24 Neighboring gene golgin subfamily A member 6-like protein 26 Neighboring gene golgin subfamily A member 6-like protein 24 Neighboring gene golgin subfamily A member 6-like protein 24 Neighboring gene si:ch211-199m3.1 Neighboring gene uncharacterized LOC110438183 Neighboring gene uncharacterized LOC103909328 Neighboring gene adenylate kinase 9

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
    • Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
    • BioProject: PRJEB1986
    • Analysis date: Fri Dec 8 19:48:10 2017

    Bibliography

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    Related articles in PubMed

    1. Analysis of gene function and visualization of cilia-generated fluid flow in Kupffer's vesicle. Wang G, et al. J Vis Exp, 2013 Mar 31. PMID 23567922, Free PMC Article
    2. Regional cell shape changes control form and function of Kupffer's vesicle in the zebrafish embryo. Wang G, et al. Dev Biol, 2012 Oct 1. PMID 22841644, Free PMC Article
    3. The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity. Lisowska J, et al. J Cell Sci, 2018 Aug 13. PMID 30030370
    4. Actin-Cytoskeleton- and Rock-Mediated INM Are Required for Photoreceptor Regeneration in the Adult Zebrafish Retina. Lahne M, et al. J Neurosci, 2015 Nov 25. PMID 26609156, Free PMC Article
    5. Yolk syncytial layer formation is a failure of cytokinesis mediated by Rock1 function in the early zebrafish embryo. Chu LT, et al. Biol Open, 2012 Aug 15. PMID 23213468, Free PMC Article

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by ZFIN

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables small GTPase binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within Kupffer's vesicle development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within Rho protein signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within actin cytoskeleton organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within epithelial cilium movement involved in determination of left/right asymmetry IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within heart morphogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of centrosome duplication IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within protein phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of actin cytoskeleton organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within smooth muscle contraction IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    rho-associated protein kinase 2b
    Names
    rho-kinase 2
    NP_001087216.1
    XP_017207922.1
    XP_017207923.1
    XP_021324031.1
    XP_021324032.1
    XP_021324035.1
    XP_021324036.1
    XP_068071478.1
    XP_068071479.1
    XP_068071480.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001093747.2 → NP_001087216.1  rho-associated protein kinase 2b

      See identical proteins and their annotated locations for NP_001087216.1

      Status: PROVISIONAL

      Source sequence(s)
      AL929220, BX571790
      UniProtKB/TrEMBL
      A0A8M1NDD8, A0A8M9PH50, F1R417
      Conserved Domains (7) summary
      smart00220
      Location:78 → 340
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      COG1196
      Location:432 → 1097
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      cd00029
      Location:1253 → 1307
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd01242
      Location:1145 → 1249
      PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain
      cd05596
      Location:51 → 402
      STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase
      pfam08912
      Location:967 → 1034
      Rho_Binding; Rho Binding
      cl00087
      Location:494 → 560
      HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

    RefSeqs of Annotated Genomes: GCF_000002035.6-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCz11 Primary Assembly

    Genomic

    1. NC_007131.7 Reference GRCz11 Primary Assembly

      Range
      33575452..33705224 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_021468361.2 → XP_021324036.1  rho-associated protein kinase 2b isoform X8

      UniProtKB/TrEMBL
      A0A8M6Z372, A0A8M9PP57
      Conserved Domains (3) summary
      cd05596
      Location:51 → 402
      STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase
      cl21453
      Location:1161 → 1346
      PKc_like; Protein Kinases, catalytic domain
      cl25732
      Location:432 → 1097
      SMC_N; RecF/RecN/SMC N terminal domain

    2. XM_021468360.2 → XP_021324035.1  rho-associated protein kinase 2b isoform X6

      UniProtKB/TrEMBL
      A0A8M9PH50, A0A8M9PSI1
      Conserved Domains (3) summary
      cd05596
      Location:51 → 402
      STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase
      cl21453
      Location:1228 → 1413
      PKc_like; Protein Kinases, catalytic domain
      cl25732
      Location:432 → 1097
      SMC_N; RecF/RecN/SMC N terminal domain

    3. XM_068215379.1 → XP_068071480.1  rho-associated protein kinase 2b isoform X9

      UniProtKB/TrEMBL
      A0A8M6Z372

    4. XM_068215377.1 → XP_068071478.1  rho-associated protein kinase 2b isoform X4

      UniProtKB/TrEMBL
      A0A8M9PH50

    5. XM_068215378.1 → XP_068071479.1  rho-associated protein kinase 2b isoform X7

      UniProtKB/TrEMBL
      A0A8M9PH50
      Related
      ENSDARP00000136393.1, ENSDART00000157427.2

    6. XM_017352434.3 → XP_017207923.1  rho-associated protein kinase 2b isoform X5

      UniProtKB/TrEMBL
      A0A8M6YWK7, A0A8M9PH50
      Conserved Domains (7) summary
      cd11638
      Location:494 → 560
      HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2
      smart00220
      Location:78 → 340
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      COG1196
      Location:432 → 1097
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      cd00029
      Location:1253 → 1307
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd01242
      Location:1145 → 1249
      PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain
      cd05596
      Location:51 → 402
      STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase
      pfam08912
      Location:967 → 1034
      Rho_Binding; Rho Binding

    7. XM_017352433.3 → XP_017207922.1  rho-associated protein kinase 2b isoform X3

      UniProtKB/TrEMBL
      A0A8M6YUR0, A0A8M9PH50
      Conserved Domains (7) summary
      cd11638
      Location:494 → 560
      HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2
      smart00220
      Location:78 → 340
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      COG1196
      Location:432 → 1097
      Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
      cd00029
      Location:1320 → 1374
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd01242
      Location:1212 → 1316
      PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain
      cd05596
      Location:51 → 402
      STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase
      pfam08912
      Location:967 → 1034
      Rho_Binding; Rho Binding

    8. XM_021468356.2 → XP_021324031.1  rho-associated protein kinase 2b isoform X1

      UniProtKB/TrEMBL
      A0A8M9PH50, A0A8M9PP53
      Conserved Domains (4) summary
      cd00029
      Location:1320 → 1374
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd01242
      Location:1212 → 1316
      PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain
      cd05596
      Location:51 → 402
      STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase
      cl25732
      Location:432 → 1097
      SMC_N; RecF/RecN/SMC N terminal domain

    9. XM_021468357.2 → XP_021324032.1  rho-associated protein kinase 2b isoform X2

      UniProtKB/TrEMBL
      A0A8M9PBK6, A0A8M9PH50
      Conserved Domains (4) summary
      cd00029
      Location:1320 → 1374
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd01242
      Location:1212 → 1316
      PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain
      cd05596
      Location:51 → 402
      STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase
      cl25732
      Location:432 → 1097
      SMC_N; RecF/RecN/SMC N terminal domain

    RNA

    1. XR_011007668.1 RNA Sequence

    2. XR_011007667.1 RNA Sequence

    3. XR_011007666.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version

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