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    Ano4 anoctamin 4 [ Mus musculus (house mouse) ]

    Gene ID: 320091, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Ano4provided by MGI
    Official Full Name
    anoctamin 4provided by MGI
    Primary source
    MGI:MGI:2443344
    See related
    Ensembl:ENSMUSG00000035189 AllianceGenome:MGI:2443344
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Gm65; Tmem16d; A330096O15Rik
    Summary
    Enables phospholipid scramblase activity. Acts upstream of or within calcium activated phospholipid scrambling and establishment of localization in cell. Predicted to be active in plasma membrane. Is expressed in dorsal root ganglion and trigeminal ganglion. Orthologous to human ANO4 (anoctamin 4). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in ovary adult (RPKM 7.1), frontal lobe adult (RPKM 4.2) and 8 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Ano4 in Genome Data Viewer
    Location:
    10 C1- C2; 10 44.42 cM
    Exon count:
    32
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 10 NC_000076.7 (88784794..89181059, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 10 NC_000076.6 (88948932..89345205, complement)

    Chromosome 10 - NC_000076.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_27512 Neighboring gene UTP20 small subunit processome component Neighboring gene STARR-seq mESC enhancer starr_27513 Neighboring gene solute carrier family 5 (iodide transporter), member 8 Neighboring gene STARR-seq mESC enhancer starr_27514 Neighboring gene STARR-seq mESC enhancer starr_27515 Neighboring gene predicted gene, 30769 Neighboring gene STARR-seq mESC enhancer starr_27517 Neighboring gene STARR-positive B cell enhancer ABC_E6898 Neighboring gene STARR-seq mESC enhancer starr_27520 Neighboring gene STARR-positive B cell enhancer ABC_E1404 Neighboring gene STARR-seq mESC enhancer starr_27521 Neighboring gene growth arrest-specific 2 like 3 Neighboring gene nuclear receptor subfamily 1, group H, member 4

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Anoctamin 4 channel currents activate glucose-inhibited neurons in the mouse ventromedial hypothalamus during hypoglycemia. Tu L, et al. J Clin Invest, 2023 Jul 17. PMID 37261917, Free PMC Article
    2. Anoctamin-4 is a bona fide Ca(2+)-dependent non-selective cation channel. Reichhart N, et al. Sci Rep, 2019 Feb 19. PMID 30783137, Free PMC Article
    3. ANOs 3-7 in the anoctamin/Tmem16 Cl- channel family are intracellular proteins. Duran C, et al. Am J Physiol Cell Physiol, 2012 Feb 1. PMID 22075693, Free PMC Article
    4. Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. Suzuki J, et al. J Biol Chem, 2013 May 10. PMID 23532839, Free PMC Article
    5. Wnt signaling regulates ion channel expression to promote smooth muscle and cartilage formation in developing mouse trachea. Russell NX, et al. Am J Physiol Lung Cell Mol Physiol, 2023 Dec 1. PMID 37873566, Free PMC Article

    See all (15) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Anoctamin 4 channel currents activate glucose-inhibited neurons in the mouse ventromedial hypothalamus during hypoglycemia.
      Title: Anoctamin 4 channel currents activate glucose-inhibited neurons in the mouse ventromedial hypothalamus during hypoglycemia.
    2. Anoctamin-4 is a bona fide Ca(2+)-dependent non-selective cation channel.
      Title: Anoctamin-4 is a bona fide Ca(2+)-dependent non-selective cation channel.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (5) 

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables chloride channel activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    NOT enables intracellularly calcium-gated chloride channel activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables intracellularly calcium-gated chloride channel activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables phospholipid scramblase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein dimerization activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within calcium activated galactosylceramide scrambling IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within calcium activated phosphatidylcholine scrambling IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within calcium activated phosphatidylserine scrambling IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in chloride transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in chloride transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chloride transmembrane transport ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within establishment of localization in cell IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    anoctamin-4
    Names
    transmembrane protein 16D (eight membrane-spanning domains)

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001277188.1 → NP_001264117.1  anoctamin-4

      See identical proteins and their annotated locations for NP_001264117.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the supported protein.
      Source sequence(s)
      AI463477, AK078364, BC065114, CF535597
      Consensus CDS
      CCDS24114.2
      UniProtKB/Swiss-Prot
      Q8C5H1, Q8CA37
      Related
      ENSMUSP00000138193.2, ENSMUST00000182341.8
      Conserved Domains (2) summary
      pfam04547
      Location:339 → 920
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:111 → 336
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    RNA

    1. NR_102339.1 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an internal exon and uses an alternate splice site in the 5' region, compared to variant 1. This variant is represented as non-coding because the use of the expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AI463477, BC065114
      Related
      ENSMUST00000182462.8

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000076.7 Reference GRCm39 C57BL/6J

      Range
      88784794..89181059 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006513757.5 → XP_006513820.1  anoctamin-4 isoform X3

      Conserved Domains (2) summary
      pfam04547
      Location:503 → 1084
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:275 → 500
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    2. XM_006513756.5 → XP_006513819.1  anoctamin-4 isoform X2

      Conserved Domains (2) summary
      pfam04547
      Location:523 → 1104
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:310 → 520
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    3. XM_006513755.5 → XP_006513818.1  anoctamin-4 isoform X1

      Conserved Domains (2) summary
      pfam04547
      Location:538 → 1119
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:310 → 535
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    4. XM_036155818.1 → XP_036011711.1  anoctamin-4 isoform X6

      Conserved Domains (2) summary
      pfam04547
      Location:168 → 749
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:26 → 165
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    5. XM_011243478.3 → XP_011241780.1  anoctamin-4 isoform X6

      See identical proteins and their annotated locations for XP_011241780.1

      Conserved Domains (2) summary
      pfam04547
      Location:168 → 749
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:26 → 165
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    6. XM_006513759.5 → XP_006513822.1  anoctamin-4 isoform X5

      UniProtKB/TrEMBL
      S4R1L3
      Related
      ENSMUSP00000138268.2, ENSMUST00000182613.8
      Conserved Domains (2) summary
      pfam04547
      Location:304 → 885
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:76 → 301
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    7. XM_030245124.1 → XP_030100984.1  anoctamin-4 isoform X6

      Conserved Domains (2) summary
      pfam04547
      Location:168 → 749
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:26 → 165
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    8. XM_030245123.2 → XP_030100983.1  anoctamin-4 isoform X4

      UniProtKB/Swiss-Prot
      Q8C5H1, Q8CA37
      Conserved Domains (2) summary
      pfam04547
      Location:339 → 920
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:111 → 336
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    9. XM_036155817.1 → XP_036011710.1  anoctamin-4 isoform X5

      UniProtKB/TrEMBL
      S4R1L3
      Related
      ENSMUSP00000138325.2, ENSMUST00000182790.8
      Conserved Domains (2) summary
      pfam04547
      Location:304 → 885
      Anoctamin; Calcium-activated chloride channel
      pfam16178
      Location:76 → 301
      Anoct_dimer; dimerization domain of Ca+-activated chloride-channel, anoctamin

    RNA

    1. XR_003948705.2 RNA Sequence

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_178773.4: Suppressed sequence

      Description
      NM_178773.4: This RefSeq was permanently suppressed because the transcript is a nonsense-mediated mRNA decay (NMD) candidate when it uses a supported upstream start codon.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8C5H1.2 GenPept UniProtKB/Swiss-Prot:Q8C5H1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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