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    OTP51 endonuclease [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 816078, updated on 18-Sep-2024

    Summary

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    Official Symbol
    OTP51
    Official Full Name
    endonuclease
    Primary source
    TAIR:AT2G15820
    Locus tag
    AT2G15820
    See related
    Araport:AT2G15820
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    F19G14.18; F19G14_18; ORGANELLE TRANSCRIPT PROCESSING 51
    Summary
    Encodes a protein that promotes splicing of type II introns. otp51 mutants fail to splice intron 2 of plastid ycf3 transcripts, a factor required for the assembly of Photosystem I. Therefore, homozygous otp51 mutants have profound photosynthetic defects and can only survive in sucrose-supplemented in vitro cultures under low light conditions. OTP51 may also be involved in splicing several other transcripts and precursor forms of the trnL, trnG, trnI, and trnA transcripts also accumulate in otp51 mutants. Although OTP51 shares some homology with DNA endonucleases, it lacks key catalytic residues suggesting that it does not participate in DNA cleavage.
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    Genomic context

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    See OTP51 in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    2
    Sequence:
    Chromosome: 2; NC_003071.7 (6888540..6891409, complement)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene pseudo Neighboring gene uncharacterized protein Neighboring gene miscRNA Neighboring gene miscRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The pentatricopeptide repeat gene OTP51 with two LAGLIDADG motifs is required for the cis-splicing of plastid ycf3 intron 2 in Arabidopsis thaliana. de Longevialle AF, et al. Plant J, 2008 Oct. PMID 18557832
    2. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana. Lin X, et al. Nature, 1999 Dec 16. PMID 10617197

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. OTP51 is an organelle splicing factor required for splicing of plastid ycf3 intron 2, a photosystem I assembly protein.
      Title: The pentatricopeptide repeat gene OTP51 with two LAGLIDADG motifs is required for the cis-splicing of plastid ycf3 intron 2 in Arabidopsis thaliana.
    Submit: New GeneRIF Correction

    General gene information

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    NM_127144.7

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within Group II intron splicing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within chloroplast mRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within photosystem I assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within tRNA splicing, via endonucleolytic cleavage and ligation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    endonuclease
    NP_565382.4
    • ORGANELLE TRANSCRIPT PROCESSING 51 (OTP51); FUNCTIONS IN: endonuclease activity; INVOLVED IN: photosystem I assembly, nuclear mRNA cis splicing, via spliceosome, Group II intron splicing; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pentatricopeptide repeat (InterPro:IPR002885), LAGLIDADG DNA endonuclease (InterPro:IPR004860); BEST Arabidopsis thaliana protein match is: Pentatricopeptide repeat (PPR) superfamily protein (TAIR:AT5G61990.1); Has 8373 Blast hits to 4744 proteins in 190 species: Archae - 0; Bacteria - 4; Metazoa - 37; Fungi - 65; Plants - 8172; Viruses - 0; Other Eukaryotes - 95 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      6888540..6891409 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_127144.7 → NP_565382.4  endonuclease [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_565382.4

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q0WM50, Q93ZT4, Q9XIL5
      UniProtKB/TrEMBL
      A0A5S9WYB7, A0A654ET70
      Conserved Domains (4) summary
      COG0457
      Location:375 → 555
      TPR; Tetratricopeptide (TPR) repeat [General function prediction only]
      sd00004
      Location:464 → 490
      PPR; PPR repeat [structural motif]
      pfam03161
      Location:648 → 795
      LAGLIDADG_2; LAGLIDADG DNA endonuclease family
      pfam13041
      Location:533 → 576
      PPR_2; PPR repeat family
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9XIL5.3

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