Fhl5 four and a half LIM domains 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Fhl5provided by MGI
Official Full Name: four and a half LIM domains 5provided by MGI
Primary source: MGI:MGI:1913192
See related: Ensembl:ENSMUSG00000028259 AllianceGenome:MGI:1913192
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Act; 1700027G07Rik
Summary: Enables transcription coactivator activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Predicted to be active in Z disc and nucleus. Is expressed in central nervous system; genitourinary system; heart; and otocyst. Orthologous to human FHL5 (four and a half LIM domains 5). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward testis adult (RPKM 61.6) See more
Orthologs: human all
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Genomic context

Location:: 4 A3; 4 10.58 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (25199907..25242852, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (25199907..25242852, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
Title: Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
Fhl5 is not essential for testicular gene expression to a level detected by microarray-based measurements.
Title: Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.
Results indicate that numerous postmeiotic genes under CREM (cAMP-responsive element modulator) control require the coactivator function of ACT (activator of CREM) in testis.
Title: Abnormal sperm in mice with targeted deletion of the act (activator of cAMP-responsive element modulator in testis) gene.
ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells; The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT
Title: CREM-dependent transcription in male germ cells controlled by a kinesin.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: four and a half LIM domains protein 5

Names: FHL-5; LIM-only protein ACT; activator of CREM in testis; activator of cAMP-responsive element modulator in testis

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001355498.2 → NP_001342427.1 four and a half LIM domains protein 5

Status: VALIDATED

Description

Transcript Variant: This variant (2) encodes the same protein as variants 1 and 3.

Source sequence(s)

AL772199, AL935267

Consensus CDS

CCDS18009.1

UniProtKB/Swiss-Prot

Q9WTX7

Conserved Domains (4) summary

cd09343
Location:37 → 95

LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)

cd09347
Location:222 → 277

LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)

cd09428
Location:102 → 155

LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)

cl02475
Location:163 → 214

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
NM_001379528.1 → NP_001366457.1 four and a half LIM domains protein 5

Status: VALIDATED

Description

Transcript Variant: This variant (3) represents the longest transcript. All three variants encode the same protein.

Source sequence(s)

AL772199, AL935267

UniProtKB/Swiss-Prot

Q9WTX7

Related

ENSMUSP00000103839.2, ENSMUST00000108204.2

Conserved Domains (4) summary

cd09343
Location:37 → 95

LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)

cd09347
Location:222 → 277

LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)

cd09428
Location:102 → 155

LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)

cl02475
Location:163 → 214

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers
NM_021318.5 → NP_067293.1 four and a half LIM domains protein 5

See identical proteins and their annotated locations for NP_067293.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3.

Source sequence(s)

AL772199, AL935267

Consensus CDS

CCDS18009.1

UniProtKB/Swiss-Prot

Q9WTX7

Related

ENSMUSP00000029922.8, ENSMUST00000029922.14

Conserved Domains (4) summary

cd09343
Location:37 → 95

LIM1_FHL; The first LIM domain of Four and a half LIM domains protein (FHL)

cd09347
Location:222 → 277

LIM4_FHL; The fourth LIM domain of Four and a half LIM domains protein (FHL)

cd09428
Location:102 → 155

LIM2_FHL5; The second LIM domain of Four and a half LIM domains protein 5 (FHL5)

cl02475
Location:163 → 214

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers