SFR2 Glycosyl hydrolase superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: SFR2
Official Full Name: Glycosyl hydrolase superfamily protein
Primary source: TAIR:AT3G06510
Locus tag: AT3G06510
See related: Araport:AT3G06510
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATSFR2; F5E6.16; F5E6_16; SENSITIVE TO FREEZING 2
Summary: Encodes a protein with beta-glucosidase and galactosyltransferase activity, mutants show increased sensitivity to freezing. Though it is classified as a family I glycosyl hydrolase, it has no hydrolase activity in vitro.
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Genomic context

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See SFR2 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 10

Sequence:: Chromosome: 3; NC_003074.8 (2016246..2020103)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

SFR2 activation during protoplast isolation correlates with cytosolic acidification.
Title: An Arabidopsis protoplast isolation method reduces cytosolic acidification and activation of the chloroplast stress sensor SENSITIVE TO FREEZING 2.
SFR2 is activated by changes in cytosolic pH and Mg(2+).
Title: Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2.
SFR2 and TAG biosynthetic enzyme activity lead to removal of monogalactolipids from chloroplast envelope membrane, changing ratio of bilayer- to non-bilayer-forming membrane lipids; SFR2-based mechanism stabilizes membranes during freezing
Title: Freezing tolerance in plants requires lipid remodeling at the outer chloroplast membrane.
SFR2 protects chloroplasts from freeze-induced damage, and is localized to the chloroplast envelope.
Title: A role for SENSITIVE TO FREEZING2 in protecting chloroplasts against freeze-induced damage in Arabidopsis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001125119.2

Supported by ESTs:

NM_111527.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables 1,4-alpha-glucan branching enzyme activity	IEA Inferred from Electronic Annotation more info
enables beta-glucosidase activity	IDA Inferred from Direct Assay more info	PubMed
enables cellobiose glucosidase activity	IDA Inferred from Direct Assay more info	PubMed
enables galactolipid galactosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables galactosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glycosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
NOT enables hydrolase activity, acting on glycosyl bonds	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to cold	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to cold	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to freezing	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to freezing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	HDA more info	PubMed
located_in chloroplast envelope	HDA more info	PubMed
located_in chloroplast envelope	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast outer membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in plasma membrane	ISM Inferred from Sequence Model more info
located_in plastid	HDA more info	PubMed

General protein information

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Preferred Names: Glycosyl hydrolase superfamily protein

NP_001118591.1

SENSITIVE TO FREEZING 2 (SFR2); FUNCTIONS IN: beta-glucosidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to freezing; LOCATED IN: chloroplast outer membrane, chloroplast, chloroplast envelope; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 1 (InterPro:IPR001360), Glycoside hydrolase, family 1, active site (InterPro:IPR018120), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta glucosidase 27 (TAIR:AT3G60120.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_566285.1

SENSITIVE TO FREEZING 2 (SFR2); FUNCTIONS IN: beta-glucosidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to freezing; LOCATED IN: chloroplast outer membrane, chloroplast, chloroplast envelope; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 1 (InterPro:IPR001360), Glycoside hydrolase, family 1, active site (InterPro:IPR018120), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta glucosidase 27 (TAIR:AT3G60120.1); Has 11582 Blast hits to 10595 proteins in 1458 species: Archae - 144; Bacteria - 8179; Metazoa - 842; Fungi - 193; Plants - 1310; Viruses - 0; Other Eukaryotes - 914 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

2016246..2020103

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_111527.3 → NP_566285.1 Glycosyl hydrolase superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_566285.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8LAN2, Q8LGU4, Q8LGU5, Q8W578, Q93Y07, Q9C8Z2, Q9SDL5

UniProtKB/TrEMBL

A0A178VES5, A0A5S9XA22

Conserved Domains (1) summary

cl40694
Location:161 → 512

BglB; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase [Carbohydrate transport and metabolism]
NM_001125119.2 → NP_001118591.1 Glycosyl hydrolase superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001118591.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q93Y07

Conserved Domains (1) summary

cl40694
Location:161 → 546

BglB; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase [Carbohydrate transport and metabolism]