smo smoothened, frizzled class receptor [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: smoprovided by ZNC
Official Full Name: smoothened, frizzled class receptorprovided by ZNC
Primary source: ZFIN:ZDB-GENE-980526-89
See related: Ensembl:ENSDARG00000002952 AllianceGenome:ZFIN:ZDB-GENE-980526-89
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: smu; smoh; CHUNP6862; wu:fb70b01; wu:fc39h03
Summary: Enables oxysterol binding activity. Involved in exocrine pancreas development. Acts upstream of or within several processes, including circulatory system development; embryonic organ development; and nervous system development. Located in cilium. Is expressed in several structures, including brain; mesoderm; pectoral fin bud; tail bud; and trunk. Human ortholog(s) of this gene implicated in basal cell carcinoma; hepatocellular carcinoma; malignant pleural mesothelioma; pancreatic cancer; and pancreatic ductal carcinoma. Orthologous to human SMO (smoothened, frizzled class receptor). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 4

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	4	NC_007115.7 (14971496..14977525)
105	previous assembly	GRCz10 (GCF_000002035.5)	4	NC_007115.6 (14972741..14978770)

Chromosome 4 - NC_007115.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The development of zebrafish pancreas affected by deficiency of Hedgehog signaling.
Title: The development of zebrafish pancreas affected by deficiency of Hedgehog signaling.
Our study implicates PDE4 as a target for Hh inhibition, suggests an improved strategy for Hh-dependent cancer therapy, and identifies a unique probe of downstream-of-Smo Hh modulation.
Title: An in vivo chemical genetic screen identifies phosphodiesterase 4 as a pharmacological target for hedgehog signaling inhibition.
The cysteine-rich SMO domain is required for signaling in response to native Hedgehog ligands, showing that it is an important regulatory module for Smo activation.
Title: Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling.
Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling.
Title: Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling.
Fine coordination of smoothened activity by the miR-30 family allows the correct specification and differentiation of distinct muscle cell types during zebrafish embryonic development.
Title: The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development.
These data indicate that the extracellular domain, previously thought to be dispensable for vertebrate Smoothened function, both regulates Smo ciliary localization and is essential for high-level Hedgehog signaling.
Title: The extracellular domain of Smoothened regulates ciliary localization and is required for high-level Hh signaling.
neither maternal nor zygotic Smo is required for PGC migration in zebrafish embryo. Cyclopamine instead acts independently of Smo to decrease motility of PGCs, in part by dysregulating cell adhesion and uncoupling cell polarization and translocation.
Title: Germ cell migration in zebrafish is cyclopamine-sensitive but Smoothened-independent.
Data suggest that a G protein-coupled receptor kinase, perhaps GRK2 or 3, functions as a vertebrate kinase for Smoothened, promoting Hedgehog signal transduction during early development.
Title: Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase.
Hhip regulates muscle development both by sequestering Hedgehog and by modulating localization of Smoothened
Title: Hhip regulates zebrafish muscle development by both sequestering Hedgehog and modulating localization of Smoothened.

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General gene information

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Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables oxysterol binding	IDA Inferred from Direct Assay more info	PubMed
enables patched binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase A catalytic subunit binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein sequestering activity	ISS Inferred from Sequence or Structural Similarity more info
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within adenohypophysis development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within angioblast cell migration from lateral mesoderm to midline	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within anterior/posterior pattern specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within arterial endothelial cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cardiac ventricle development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cardioblast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cartilage development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within caudal fin morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell surface receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in central nervous system development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within central nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cerebellum morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in commissural neuron axon guidance	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within commissural neuron axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within diencephalon development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within dorsal aorta morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic camera-type eye development	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within embryonic digestive tract morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic neurocranium morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic pattern specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic viscerocranium morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endocardial cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endocrine pancreas development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endocrine system development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within epithelial tube formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within exocrine pancreas development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocrine pancreas development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within floor plate formation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within forebrain development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within forebrain dorsal/ventral pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glial cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within habenula development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart looping	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hindbrain development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inner ear development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inner ear morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within muscle cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within muscle organ development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within myofibril assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural crest cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural plate morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural plate pattern specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within oligodendrocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within otolith morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pancreas development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pattern specification process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within receptor clustering	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within retina development in camera-type eye	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within retinal ganglion cell axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within semicircular canal morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within skeletal muscle fiber development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within smooth muscle tissue development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in smoothened signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in smoothened signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within somatic motor neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within somite development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spinal cord motor neuron cell fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spinal cord motor neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within striated muscle cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within swim bladder development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within swim bladder morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within telencephalon development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vasculogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
is_active_in cilium	IBA Inferred from Biological aspect of Ancestor more info
located_in cilium	IDA Inferred from Direct Assay more info	PubMed
is_active_in cilium	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cilium	ISS Inferred from Sequence or Structural Similarity more info	PubMed
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: protein smoothened

Names: slow muscle omitted; smoothened homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_131027.2 → NP_571102.2 protein smoothened precursor

Status: VALIDATED

Source sequence(s)

BX571796, CT735752

UniProtKB/Swiss-Prot

A0A8N1Z117, Q5RH73, Q90X26

Related

ENSDARP00000013486.5, ENSDART00000005985.7

Conserved Domains (2) summary

cd07451
Location:44 → 175

CRD_SMO; Cysteine-rich domain of the smoothened receptor (Smo) integral membrane protein

pfam01534
Location:200 → 528

Frizzled; Frizzled/Smoothened family membrane region