U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    PHO2 phosphate 2 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 817943, updated on 18-Sep-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    PHO2
    Official Full Name
    phosphate 2
    Primary source
    TAIR:AT2G33770
    Locus tag
    AT2G33770
    See related
    Araport:AT2G33770
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATUBC24; phosphate 2; T1B8.8; T1B8_8; UBC24; UBIQUITIN-CONJUGATING ENZYME 24
    Summary
    Encodes a ubiquitin-conjugating E2 enzyme. UBC24 mRNA accumulation is suppressed by miR399f, miR399b and miR399c. Involved in phosphate starvation response and mediates degradation of PHO1 and PHT1s at endomembrane.
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    See PHO2 in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    9
    Sequence:
    Chromosome: 2; NC_003071.7 (14277558..14283113, complement)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Nitrogen regulatory PII-like, alpha/beta Neighboring gene purine permease 2 Neighboring gene Pentatricopeptide repeat (PPR) superfamily protein Neighboring gene miscRNA Neighboring gene ncRNA Neighboring gene ralf-like 19

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Regulatory network of microRNA399 and PHO2 by systemic signaling. Lin SI, et al. Plant Physiol, 2008 Jun. PMID 18390805, Free PMC Article
    2. pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene. Aung K, et al. Plant Physiol, 2006 Jul. PMID 16679417, Free PMC Article
    3. Uptake and translocation of phosphate by pho2 mutant and wild-type seedlings of Arabidopsis thaliana. Dong B, et al. Planta, 1998 Jun. PMID 9637070
    4. Regulation of stomatal development by stomatal lineage miRNAs. Zhu J, et al. Proc Natl Acad Sci U S A, 2020 Mar 17. PMID 32123075, Free PMC Article
    5. NITROGEN LIMITATION ADAPTATION recruits PHOSPHATE2 to target the phosphate transporter PT2 for degradation during the regulation of Arabidopsis phosphate homeostasis. Park BS, et al. Plant Cell, 2014 Jan. PMID 24474629, Free PMC Article

    See all (14) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Study shows that miR399-mediated PHO2 regulation, involved in phosphate homeostasis, contributes to the control of stomatal development in Arabidopsis.
      Title: Regulation of stomatal development by stomatal lineage miRNAs.
    2. PHR1 is a key factor for metabolic reprogramming during phosphorus (P) limitation. The effects of pho2 or microRNA399 overexpression were comparatively minor.
      Title: Identification of primary and secondary metabolites with phosphorus status-dependent abundance in Arabidopsis, and of the transcription factor PHR1 as a major regulator of metabolic changes during phosphorus limitation.
    3. NLA is an E3 ligase that specifically requires UBC24 for polyubiquitination in Arabidopsis thaliana.
      Title: NITROGEN LIMITATION ADAPTATION recruits PHOSPHATE2 to target the phosphate transporter PT2 for degradation during the regulation of Arabidopsis phosphate homeostasis.
    4. PHO2 negatively regulates the protein abundance of PHF1 and PHT1 proteins under Pi-sufficient conditions
      Title: Identification of downstream components of ubiquitin-conjugating enzyme PHOSPHATE2 by quantitative membrane proteomics in Arabidopsis roots.
    5. The phosphate-accumulating mutants siz1 and pho2 reduce the uptake and accumulation of Na(+), leading to enhanced salt tolerance, and that, genetically, SIZ1 and PHO2 are likely independent of SOS3-dependent salt signaling.
      Title: Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2.
    6. PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis.
      Title: PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis.
    7. Expression of TSF was increased in miR399b-overexpressing plants and pho2 mutants at 23 degrees C, suggesting that their early flowering phenotype is associated with TSF upregulation.
      Title: The role of the miR399-PHO2 module in the regulation of flowering time in response to different ambient temperatures in Arabidopsis thaliana.
    8. IPS1 overexpression results in increased accumulation of the miR-399 target PHO2 mRNA and, concomitantly, in reduced shoot Pi content; engineering of IPS1 to be cleavable abolishes its inhibitory activity on miR-399.
      Title: Target mimicry provides a new mechanism for regulation of microRNA activity.
    9. Map-based cloning identified PHO2 as At2g33770. miR399 and PHO2 are in a branch of the Pi-signaling network downstream of PHR1.
      Title: PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants.
    10. A previously identified Pi overaccumulator, pho2, is caused by a single nucleotide mutation resulting in early termination within the UBC24 gene.
      Title: pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help
    NM_179887.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin conjugating enzyme activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin-protein transferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within cellular response to phosphate starvation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within phosphate ion homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within phosphate ion transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within regulation of phosphate transmembrane transport IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Golgi apparatus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    phosphate 2
    NP_850218.1
    • phosphate 2 (PHO2); CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 23 (TAIR:AT2G16920.1); Has 6405 Blast hits to 6302 proteins in 368 species: Archae - 0; Bacteria - 0; Metazoa - 2716; Fungi - 1263; Plants - 1493; Viruses - 17; Other Eukaryotes - 916 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      14277558..14283113 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_179887.3 → NP_850218.1  phosphate 2 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_850218.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      P93012, Q0WLN6, Q0WM96, Q8VY10
      UniProtKB/TrEMBL
      A0A5S9X3R7, A0A654EZY1
      Conserved Domains (1) summary
      cd00195
      Location:664 → 817
      UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8VY10.1

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Molecular Biology Databases
    Research Materials
    Tools