PCS2 phytochelatin synthase 2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PCS2
Official Full Name: phytochelatin synthase 2
Primary source: TAIR:AT1G03980
Locus tag: AT1G03980
See related: Araport:AT1G03980
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATPCS2; F21M11.9; F21M11_9; phytochelatin synthase 2
Summary: Encodes a protein with phytochelatin synthase activity which binds Cd2+ and Cd-glutathione complexes with high affinity. The protein has been postulated to be involved in Cd2+ tolerance. AtPCS2 expression appears to be less than that of AtPCS1, explaining the inability of endogenous AtPCS2 to substitute for AtPCS1 in the cad1-3 mutant (AtPCS1 null).
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Genomic context

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See PCS2 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 9

Sequence:: Chromosome: 1; NC_003070.9 (1019311..1022108, complement)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Arabidopsis transcription factor WRKY45 confers cadmium tolerance via activating PCS1 and PCS2 expression.
Title: Arabidopsis transcription factor WRKY45 confers cadmium tolerance via activating PCS1 and PCS2 expression.
AtPCS2 plays a positive role in seed germination and seedling growth under salt stress through a series of indirect effects that are likely involved in H2O2 scavenging, regulation of osmotic adjustment and ion homeostasis.
Title: Overexpression of phytochelatin synthase AtPCS2 enhances salt tolerance in Arabidopsis thaliana.
Constitutive AtPCS2-dependent phytochelatin synthesis suggests a physiological role of AtPCS2 other than metal detoxification.
Title: Arabidopsis thaliana phytochelatin synthase 2 is constitutively active in vivo and can rescue the growth defect of the PCS1-deficient cad1-3 mutant on Cd-contaminated soil.
expression of AtPCS2 is weak compared to AtPCS1 in both roots and shoots of transgenic Arabidopsis; it was not capable of fully complementing the AtPCS1-defective cad1-3 mutant for cadmium resistance
Title: Expression of Arabidopsis phytochelatin synthase 2 is too low to complement an AtPCS1-defective Cad1-3 mutant.
papain Cys protease superfamily member which contains an auxiliary metal-sensing C-terminal domain that undergoes secondary gamma-Glu-Cys acylation
Title: Mutagenic definition of a papain-like catalytic triad, sufficiency of the N-terminal domain for single-site core catalytic enzyme acylation, and C-terminal domain for augmentative metal activation of a eukaryotic phytochelatin synthase.

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Pathways from PubChem

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General gene information

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Homology

Orthologs from OrthoDB

NM_001331453.1

Supported by ESTs:

NM_001331455.1

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables glutathione gamma-glutamylcysteinyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutathione gamma-glutamylcysteinyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within phytochelatin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phytochelatin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in response to metal ion	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: phytochelatin synthase 2

NP_001318916.1

phytochelatin synthase 2 (PCS2); CONTAINS InterPro DOMAIN/s: Phytochelatin synthase, C-terminal (InterPro:IPR015407), Phytochelatin synthase (InterPro:IPR007719); BEST Arabidopsis thaliana protein match is: phytochelatin synthase 1 (PCS1) (TAIR:AT5G44070.1); Has 342 Blast hits to 342 proteins in 145 species: Archae - 0; Bacteria - 138; Metazoa - 36; Fungi - 7; Plants - 117; Viruses - 0; Other Eukaryotes - 44 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

1019311..1022108 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_100279.5 → NP_171894.1 phytochelatin synthase 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_171894.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9ZWB7

UniProtKB/TrEMBL

A0A178WLT1, A0A5S9SI26

Conserved Domains (2) summary

pfam05023
Location:7 → 212

Phytochelatin; Phytochelatin synthase

pfam09328
Location:230 → 433

Phytochelatin_C; Domain of unknown function (DUF1984)
NM_001331454.1 → NP_001322971.1 phytochelatin synthase 2 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AVN4
NM_001331453.1 → NP_001318916.1 phytochelatin synthase 2 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A2H1ZEA1
NM_001331455.1 → NP_001322970.1 phytochelatin synthase 2 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8AVJ0