TT8 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: TT8
Official Full Name: basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Primary source: TAIR:AT4G09820
Locus tag: AT4G09820
See related: Araport:AT4G09820
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtTT8; BHLH42; F17A8.170; F17A8_170; TRANSPARENT TESTA 8
Summary: TT8 is a regulation factor that acts in a concerted action with TT1, PAP1 and TTG1 on the regulation of flavonoid pathways, namely proanthocyanidin and anthocyanin biosynthesis. Affects dihydroflavonol 4-reductase gene expression. It is thought that a ternary complex composed of TT2, TT8 and TTG1 is necessary for correct expression of BAN in seed endothelium. Also important for important for marginal trichome development. It binds the promoter of both AT3G26790 and AT1G28300.
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Genomic context

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See TT8 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 7

Sequence:: Chromosome: 4; NC_003075.7 (6181881..6186523)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the C-terminal domain of the bHLH members (GLABRA3 [GL3], ENHANCER OF GLABRA3 [EGL3] and TRANSPARENT TESTA8 [TT8]) interacted with JAZs.
Title: The C-terminal domains of Arabidopsis GL3/EGL3/TT8 interact with JAZ proteins and mediate dimeric interactions.
The transcription factor TT8 is an integrator of secondary metabolism and stress response.
Title: Coordinate Regulation of Metabolite Glycosylation and Stress Hormone Biosynthesis by TT8 in Arabidopsis.
Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26Sproteasome system
Title: Proteolytic degradation of the flavonoid regulators, TRANSPARENT TESTA8 and TRANSPARENT TESTA GLABRA1, in Arabidopsis is mediated by the ubiquitin/26Sproteasome system.
These results provide a molecular model consistent with the specific and highly regulated expression of TT8.
Title: Regulation of flavonoid biosynthesis involves an unexpected complex transcriptional regulation of TT8 expression, in Arabidopsis.
Nitrogen regulation of anthocyanin biosynthesis in Arabidopsis thaliana red cells undergoes a mechanism by which nitrogen controls the expression of genes encoding both main components of the TTG1-GL3/TT8-PAP1 complex and negative regulators.
Title: Regulation of anthocyanin biosynthesis by nitrogen in TTG1-GL3/TT8-PAP1-programmed red cells of Arabidopsis thaliana.
TT8 expression is controlled by different combinations of MYB and bHLH factors in planta.
Title: TT8 controls its own expression in a feedback regulation involving TTG1 and homologous MYB and bHLH factors, allowing a strong and cell-specific accumulation of flavonoids in Arabidopsis thaliana.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_117050.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IEA Inferred from Electronic Annotation more info
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within jasmonic acid mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of anthocyanin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of flavonoid biosynthetic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within regulation of proanthocyanidin biosynthetic process	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within seed coat development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within seed development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within trichome differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: basic helix-loop-helix (bHLH) DNA-binding superfamily protein

NP_192720.2

TRANSPARENT TESTA 8 (TT8); FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: trichome differentiation, regulation of flavonoid biosynthetic process, regulation of proanthocyanidin biosynthetic process; LOCATED IN: nucleus; EXPRESSED IN: stem, hypocotyl, micropylar endosperm; EXPRESSED DURING: C globular stage; CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G63650.3); Has 2828 Blast hits to 2555 proteins in 185 species: Archae - 0; Bacteria - 2; Metazoa - 59; Fungi - 73; Plants - 2682; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

6181881..6186523

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_117050.3 → NP_192720.2 basic helix-loop-helix (bHLH) DNA-binding superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_192720.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A0MF61, Q1PEA5, Q9FT81, Q9SZ97

UniProtKB/TrEMBL

A0A5S9XR40

Conserved Domains (3) summary

smart00353
Location:366 → 414

HLH; helix loop helix domain

pfam14215
Location:20 → 201

bHLH-MYC_N; bHLH-MYC and R2R3-MYB transcription factors N-terminal

cl09141
Location:446 → 509

ACT; ACT domains are commonly involved in specifically binding an amino acid or other small ligand leading to regulation of the enzyme