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    MAP65-4 microtubule-associated protein 65-4 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 825255, updated on 18-Sep-2024

    Summary

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    Official Symbol
    MAP65-4
    Official Full Name
    microtubule-associated protein 65-4
    Primary source
    TAIR:AT3G60840
    Locus tag
    AT3G60840
    See related
    Araport:AT3G60840
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    microtubule-associated protein 65-4
    Summary
    Encodes MAP65-4, a non-motor microtubule associated protein (MAP) that belongs to the evolutionarily conserved MAP65 family. MAP65-4 specifically associates with the forming mitotic spindle during prophase and with the kinetochore fibers from prometaphase to the end of anaphase. MAP65-4 cross-links microtubules and promotes microtubule bundle elongation.
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    Genomic context

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    See MAP65-4 in Genome Data Viewer
    Location:
    chromosome: 3
    Exon count:
    13
    Sequence:
    Chromosome: 3; NC_003074.8 (22477573..22480980, complement)

    Chromosome 3 - NC_003074.8Genomic Context describing neighboring genes Neighboring gene DUF1499 family protein Neighboring gene N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein Neighboring gene actin-related protein 7 Neighboring gene uncharacterized protein Neighboring gene SEC7-like guanine nucleotide exchange family protein

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003074.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Arabidopsis MAP65-4 plays a role in phragmoplast microtubule organization and marks the cortical cell division site. Li H, et al. New Phytol, 2017 Jul. PMID 28370001
    2. Arabidopsis kinetochore fiber-associated MAP65-4 cross-links microtubules and promotes microtubule bundle elongation. Fache V, et al. Plant Cell, 2010 Nov. PMID 21119057, Free PMC Article
    3. MAP65/Ase1 promote microtubule flexibility. Portran D, et al. Mol Biol Cell, 2013 Jun. PMID 23615441, Free PMC Article
    4. MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis. Caillaud MC, et al. Plant Cell, 2008 Feb. PMID 18263774, Free PMC Article
    5. The C-terminal variable region specifies the dynamic properties of Arabidopsis microtubule-associated protein MAP65 isotypes. Smertenko AP, et al. Plant Cell, 2008 Dec. PMID 19060108, Free PMC Article

    See all (8) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MAP65-4 has a role in engaging and bundling anti-parallel MTs in the phragmoplast.
      Title: Arabidopsis MAP65-4 plays a role in phragmoplast microtubule organization and marks the cortical cell division site.
    2. Data indicate that microtubules (MTs) bundle flexibility is differently regulated, depending on MAP65 cross-linker.
      Title: MAP65/Ase1 promote microtubule flexibility.
    3. Data show that Arabidopsis thaliana MAP65-4 specifically associates with the forming mitotic spindle during prophase and with the kinetochore fibers from prometaphase to the end of anaphase.
      Title: Arabidopsis kinetochore fiber-associated MAP65-4 cross-links microtubules and promotes microtubule bundle elongation.
    4. Subcellular localization of MAP65-3 and analysis of microtubule organization in the mutant demonstrated that MAP65-3 played a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs.
      Title: MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis.
    Submit: New GeneRIF Correction

    General gene information

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    NM_001340060.1

    Gene Ontology Provided by TAIR

    Process Evidence Code Pubs
    acts_upstream_of_or_within microtubule bundle formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in kinetochore microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    located_in phragmoplast HDA PubMed 
    located_in phragmoplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plant-type vacuole HDA PubMed 
    located_in preprophase band IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in spindle HDA PubMed 

    General protein information

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    Preferred Names
    microtubule-associated protein 65-4
    NP_001319808.1
    • microtubule-associated protein 65-4 (MAP65-4); INVOLVED IN: anaphase; LOCATED IN: nucleus, vacuole, preprophase band, phragmoplast, spindle; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Microtubule-associated protein, MAP65/ASE1-type (InterPro:IPR007145); BEST Arabidopsis thaliana protein match is: Microtubule associated protein (MAP65/ASE1) family protein (TAIR:AT5G51600.1); Has 9353 Blast hits to 7282 proteins in 721 species: Archae - 305; Bacteria - 1033; Metazoa - 4598; Fungi - 860; Plants - 701; Viruses - 22; Other Eukaryotes - 1834 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003074.8 Reference assembly

      Range
      22477573..22480980 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001340060.1 → NP_001319808.1  microtubule-associated protein 65-4 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      F4JD03, Q9LZY0
      UniProtKB/TrEMBL
      A0A178VIY1
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9LZY0.2

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