MSI1 Transducin/WD40 repeat-like superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: MSI1
Official Full Name: Transducin/WD40 repeat-like superfamily protein
Primary source: TAIR:AT5G58230
Locus tag: AT5G58230
See related: Araport:AT5G58230
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS MULTICOPY SUPRESSOR OF IRA1; ATMSI1; MATERNAL EFFECT EMBRYO ARREST 70; MCK7.10; MCK7_10; MEE70; MULTICOPY SUPRESSOR OF IRA1
Summary: Encodes a WD-40 repeat containing protein that functions in chromatin assembly as part of the CAF1 and FIE complex. Mutants exhibit parthenogenetic development that includes proliferation of unfertilized endosperm and embryos. In heterozygous plants 50% of embryos abort. Of the aborted embryos the early aborted class are homozygous and the later aborting lass are heterozygotes in which the defective allele is maternally transmitted. Other phenotypes include defects in ovule morphogenesis and organ initiation,as well as increased levels of heterochromatic DNA. MSI1 is needed for the transition to flowering. In Arabidopsis, the three CAF-1 subunits are encoded by FAS1, FAS2 and, most likely, MSI1, respectively. Mutations in FAS1 or FAS2 lead to increased frequency of homologous recombination and T-DNA integration in Arabidopsis. In the ovule, the MSI1 transcripts are accumulated at their highest level before fertilization and gradually decrease after fertilization. MSI is biallelically expressed, the paternall allele is expressed in the endosperm and embryo and is not imprinted. MSI1 forms a complex with RBR1 that is required for activation of the imprinted genes FIS2 and FWA. This activation is mediated by MSI1/RBR1 mediated repression of MET1.
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Genomic context

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See MSI1 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 6

Sequence:: Chromosome: 5; NC_003076.8 (23556012..23558245)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MSI1 and HDA6 function interdependently to control flowering time via chromatin modifications.
Title: MSI1 and HDA6 function interdependently to control flowering time via chromatin modifications.
Ultraviolet-B light downregulates the expression of MSI1 and CLF, two of the components of the polycomb repressive complex 2, which in consequence drives a decrease in H3K27me3 histone methylation of MIR156 and FLC genes.[MSI1]
Title: UV-B radiation delays flowering time through changes in the PRC2 complex activity and miR156 levels in Arabidopsis thaliana.
These results reveal the presence of an MSI1-HDA19 complex that fine-tunes abscissic acid signaling in Arabidopsis.
Title: The WD40 Domain Protein MSI1 Functions in a Histone Deacetylase Complex to Fine-Tune Abscisic Acid Signaling.
MSI1 serves to link PRC2 to LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), a protein that binds H3K27me3 in vitro and in vivo and is required for a functional polycomb group system.
Title: Arabidopsis MSI1 connects LHP1 to PRC2 complexes.
CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting.
Title: The Arabidopsis CUL4-DDB1 complex interacts with MSI1 and is required to maintain MEDEA parental imprinting.
Data report that MSI1 can bind to the chromatin of the drought-inducible downstream target RD20 and suggest a new role for MSI1 in the negative regulation of the Arabidopsis drought-stress response.
Title: Arabidopsis MSI1 is required for negative regulation of the response to drought stress.
Rb-dependent activation is mediated by direct transcriptional repression of MET1 during female gametogenesis.
Title: Retinoblastoma and its binding partner MSI1 control imprinting in Arabidopsis.
Data suggest that the Polycomb group protein MSI1 functions in the female gametophyte to determine seed development in plants.
Title: Polycomb group proteins function in the female gametophyte to determine seed development in plants.
These results reveal that the functions of FAS1, FAS2 and MSI1 are not restricted to meristems, but are also needed to control genome replication at multiple steps of development.
Title: Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development.
MSI1 is a novel gene which regulates flowering.
Title: Regulation of flowering time by Arabidopsis MSI1.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_125208.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription corepressor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within embryo development ending in seed dormancy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heterochromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within leaf development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within multicellular organism development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase III	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within pollen development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pollen development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of flower development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within seed coat development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within seed development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within trichome differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cul4-RING E3 ubiquitin ligase complex	ISS Inferred from Sequence or Structural Similarity more info	PubMed
part_of DRM complex	IDA Inferred from Direct Assay more info	PubMed
part_of chromatin silencing complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info
located_in nucleus	TAS Traceable Author Statement more info	PubMed
part_of protein-containing complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: Transducin/WD40 repeat-like superfamily protein

NP_200631.1

MULTICOPY SUPRESSOR OF IRA1 (MSI1); FUNCTIONS IN: protein binding; INVOLVED IN: in 13 processes; LOCATED IN: nucleus, chloroplast, chromatin remodeling complex, CUL4 RING ubiquitin ligase complex; EXPRESSED IN: 28 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Histone-binding protein RBBP4 (InterPro:IPR022052), WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), G-protein beta WD-40 repeat, region (InterPro:IPR020472), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: Transducin family protein / WD-40 repeat family protein (TAIR:AT2G16780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

23556012..23558245

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_125208.3 → NP_200631.1 Transducin/WD40 repeat-like superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_200631.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O22467

UniProtKB/TrEMBL

A0A178UPV1, A0A5S9YF26

Conserved Domains (3) summary

sd00039
Location:129 → 176

7WD40; WD40 repeat [structural motif]

pfam12265
Location:19 → 88

CAF1C_H4-bd; Histone-binding protein RBBP4 or subunit C of CAF1 complex

cl29593
Location:124 → 404

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...