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    CPNIFS chloroplastic NIFS-like cysteine desulfurase [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 837370, updated on 18-Sep-2024

    Summary

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    Official Symbol
    CPNIFS
    Official Full Name
    chloroplastic NIFS-like cysteine desulfurase
    Primary source
    TAIR:AT1G08490
    Locus tag
    AT1G08490
    See related
    Araport:AT1G08490
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATCPNIFS; ATNFS2; ATSUFS; chloroplastic NIFS-like cysteine desulfurase; CYSTEINE DESULFURASE; SUFS; T27G7.17; T27G7_17
    Summary
    Chloroplastic NifS-like protein that can catalyze the conversion of cysteine into alanine and elemental sulfur (S(0)) and of selenocysteine into alanine and elemental Se (Se(0)). Overexpression enhances selenium tolerance and accumulation.
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    Genomic context

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    See CPNIFS in Genome Data Viewer
    Location:
    chromosome: 1
    Exon count:
    9
    Sequence:
    Chromosome: 1; NC_003070.9 (2685659..2688658, complement)

    Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene Plant-specific transcription factor YABBY family protein Neighboring gene strictosidine synthase-like 3 Neighboring gene succinate dehydrogenase subunit Neighboring gene early nodulin-like protein 18 Neighboring gene fatty acyl-ACP thioesterases B

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003070.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Roret T, et al. Acta Crystallogr F Struct Biol Commun, 2014 Sep. PMID 25195888, Free PMC Article
    2. Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS. Van Hoewyk D, et al. Proc Natl Acad Sci U S A, 2007 Mar 27. PMID 17372218, Free PMC Article
    3. Overexpression of AtCpNifS enhances selenium tolerance and accumulation in Arabidopsis. Van Hoewyk D, et al. Plant Physiol, 2005 Nov. PMID 16244144, Free PMC Article
    4. The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin. Ye H, et al. Planta, 2005 Feb. PMID 15480755
    5. Characterization of a NifS-like chloroplast protein from Arabidopsis. Implications for its role in sulfur and selenium metabolism. Pilon-Smits EA, et al. Plant Physiol, 2002 Nov. PMID 12427997, Free PMC Article

    See all (8) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. overexpression of this plant NifS-like protein had a pronounced effect on plant Se metabolism
      Title: Overexpression of AtCpNifS enhances selenium tolerance and accumulation in Arabidopsis.
    2. CpNifS is necessary for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth
      Title: Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS.
    3. CpNifS was sufficient for Fe-S cluster formation in ferredoxin in the presence of cysteine and a ferrous iron salt. [CpNifS]
      Title: The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    NM_100722.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables cysteine desulfurase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables pyridoxal phosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables selenocysteine lyase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cysteine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within iron incorporation into metallo-sulfur cluster IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to selenium ion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within selenium compound metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within sulfur compound metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chloroplast ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    chloroplastic NIFS-like cysteine desulfurase
    NP_172325.2
    • chloroplastic NIFS-like cysteine desulfurase (CPNIFS); FUNCTIONS IN: transaminase activity, selenocysteine lyase activity, cysteine desulfurase activity; INVOLVED IN: iron incorporation into metallo-sulfur cluster, response to selenium ion, sulfur metabolic process, selenium metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Cysteine desulfurase, SufS (InterPro:IPR010970), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: nitrogen fixation S (NIFS)-like 1 (TAIR:AT5G65720.1); Has 22660 Blast hits to 22653 proteins in 2897 species: Archae - 350; Bacteria - 15408; Metazoa - 379; Fungi - 404; Plants - 268; Viruses - 1; Other Eukaryotes - 5850 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003070.9 Reference assembly

      Range
      2685659..2688658 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_100722.5 → NP_172325.2  chloroplastic NIFS-like cysteine desulfurase [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_172325.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q93WX6, Q9SJE3
      UniProtKB/TrEMBL
      A0A178WGK3, A0A5S9TC32
      Conserved Domains (1) summary
      PLN02855
      Location:48 → 463
      PLN02855; Bifunctional selenocysteine lyase/cysteine desulfurase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q93WX6.1

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