PHS1 dual specificity protein phosphatase family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PHS1
Official Full Name: dual specificity protein phosphatase family protein
Primary source: TAIR:AT5G23720
Locus tag: AT5G23720
See related: Araport:AT5G23720
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: MQM1.1; MQM1_1; PROPYZAMIDE-HYPERSENSITIVE 1
Summary: Encodes a protein tyrosine phosphatase Propyzamide-Hypersensitive 1 (PHS1). One of the mutant alleles, phs1-1, is hypersensitive to the microtubule-destabilizing drug propyzamide, suggesting that PHS1 may be involved in phosphorylation cascades that control the dynamics of cortical microtubules in plant cells. A second allele, phs1-3, is hypersensitive to abscisic acid, indicating a possible involvement of PHS1 in ABA signalling.
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Genomic context

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See PHS1 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 11

Sequence:: Chromosome: 5; NC_003076.8 (7998197..8002848)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results indicate that plastidal phosphorylase (PHS1) and starch synthase 4 (SS4) are differently involved in starch granule formation and do not act in series.
Title: Starch Synthase 4 and Plastidal Phosphorylase Differentially Affect Starch Granule Number and Morphology.
Reduced starch granule number per chloroplast in the DPE2/PHS1 mutant is dependent on initiation of starch degradation via GWD3.
Title: Reduced starch granule number per chloroplast in the dpe2/phs1 mutant is dependent on initiation of starch degradation.
PHS1 plays a dual role in flowering, in the photoperiodic and autonomous pathways, by modulating both CO and FLC proteins mRNA levels.
Title: The mitogen-activated protein kinase phosphatase PHS1 regulates flowering in Arabidopsis thaliana.
When grown in a light-dark regime, mesophyll chloroplasts of dpe2-1xphs1a contain a single starch granule but under continuous illumination more granules per chloroplast are formed.
Title: Double knockout mutants of Arabidopsis grown under normal conditions reveal that the plastidial phosphorylase isozyme participates in transitory starch metabolism.
PHS1 was found to encode a protein containing a deaminase-reductase domain that is involved in the riboflavin pathway.
Title: The photosensitive phs1 mutant is impaired in the riboflavin biogenesis pathway.
A nuclear export signal identified in the C-terminal tail also contributes to the robust cytoplasmic retention of PHS1.
Title: Mitogen-activated protein kinase phosphatase PHS1 is retained in the cytoplasm by nuclear extrusion signal-dependent and independent mechanisms.
Data show that the product of the PHS1 gene is a cytoplasmic protein that functions by controlling transport of RAD50 from cytoplasm to the nucleus.
Title: PHS1 regulates meiotic recombination and homologous chromosome pairing by controlling the transport of RAD50 to the nucleus.
PHS1, which encodes a dual-specificity PTP, is a negative regulator of ABA signalling.
Title: The phs1-3 mutation in a putative dual-specificity protein tyrosine phosphatase gene provokes hypersensitive responses to abscisic acid in Arabidopsis thaliana.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001036856.2

Supported by ESTs:

NM_122277.2

Supported by ESTs:

NM_180735.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine phosphatase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cortical microtubule organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cortical microtubule organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of stomatal movement	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to abscisic acid	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to abscisic acid	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: dual specificity protein phosphatase family protein

NP_001031933.1

PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1); FUNCTIONS IN: protein tyrosine/serine/threonine phosphatase activity, phosphoprotein phosphatase activity; INVOLVED IN: cortical microtubule organization, regulation of gene expression, response to abscisic acid stimulus, regulation of stomatal movement; LOCATED IN: cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Zinc finger, C2H2-like (InterPro:IPR015880), Dual-specific/protein-tyrosine phosphatase, conserved region (InterPro:IPR000387), Dual specificity phosphatase, subgroup, catalytic domain (InterPro:IPR020422), Protein kinase-like domain (InterPro:IPR011009), Protein-tyrosine phosphatase, active site (InterPro:IPR016130), Actin-fragmin kinase, catalytic (InterPro:IPR015275), Dual specificity phosphatase, catalytic domain (InterPro:IPR000340); BEST Arabidopsis thaliana protein match is: dual specificity protein phosphatase 1 (TAIR:AT3G23610.2); Has 3765 Blast hits to 3755 proteins in 331 species: Archae - 11; Bacteria - 94; Metazoa - 2032; Fungi - 332; Plants - 347; Viruses - 172; Other Eukaryotes - 777 (source: NCBI BLink).

NP_197761.2

PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1); FUNCTIONS IN: protein tyrosine/serine/threonine phosphatase activity, phosphoprotein phosphatase activity; INVOLVED IN: cortical microtubule organization, regulation of gene expression, response to abscisic acid stimulus, regulation of stomatal movement; LOCATED IN: cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein-tyrosine phosphatase, active site (InterPro:IPR016130), Dual-specific/protein-tyrosine phosphatase, conserved region (InterPro:IPR000387), Actin-fragmin kinase, catalytic (InterPro:IPR015275), Dual specificity phosphatase, catalytic domain (InterPro:IPR000340), Dual specificity phosphatase, subgroup, catalytic domain (InterPro:IPR020422), Protein kinase-like domain (InterPro:IPR011009); BEST Arabidopsis thaliana protein match is: dual specificity protein phosphatase 1 (TAIR:AT3G23610.2); Has 3732 Blast hits to 3722 proteins in 327 species: Archae - 11; Bacteria - 92; Metazoa - 2029; Fungi - 326; Plants - 343; Viruses - 172; Other Eukaryotes - 759 (source: NCBI BLink).

NP_851066.2

PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1); FUNCTIONS IN: protein tyrosine/serine/threonine phosphatase activity, phosphoprotein phosphatase activity; INVOLVED IN: cortical microtubule organization, regulation of gene expression, response to abscisic acid stimulus, regulation of stomatal movement; LOCATED IN: cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Zinc finger, C2H2-like (InterPro:IPR015880), Dual-specific/protein-tyrosine phosphatase, conserved region (InterPro:IPR000387), Dual specificity phosphatase, subgroup, catalytic domain (InterPro:IPR020422), Protein kinase-like domain (InterPro:IPR011009), Protein-tyrosine phosphatase, active site (InterPro:IPR016130), Actin-fragmin kinase, catalytic (InterPro:IPR015275), Dual specificity phosphatase, catalytic domain (InterPro:IPR000340); BEST Arabidopsis thaliana protein match is: dual specificity protein phosphatase 1 (TAIR:AT3G23610.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

7998197..8002848

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_180735.4 → NP_851066.2 dual specificity protein phosphatase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_851066.2

Status: REVIEWED

UniProtKB/Swiss-Prot

F4KE97, Q75QN6, Q9FFA8

UniProtKB/TrEMBL

A0A654G3I7, A0A7G2F9M4

Conserved Domains (2) summary

cd14498
Location:704 → 841

DSP; dual-specificity phosphatase domain

pfam09192
Location:111 → 426

Act-Frag_cataly; Actin-fragmin kinase, catalytic
NM_122277.2 → NP_197761.2 dual specificity protein phosphatase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_197761.2

Status: REVIEWED

UniProtKB/TrEMBL

Q8L613

Conserved Domains (2) summary

cd00127
Location:619 → 759

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam09192
Location:27 → 342

Act-Frag_cataly; Actin-fragmin kinase, catalytic
NM_001036856.2 → NP_001031933.1 dual specificity protein phosphatase family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031933.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A7G2F9M4, F4KE96

Conserved Domains (2) summary

cd00127
Location:694 → 834

DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...

pfam09192
Location:102 → 417

Act-Frag_cataly; Actin-fragmin kinase, catalytic