PUMP1 plant uncoupling mitochondrial protein 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PUMP1
Official Full Name: plant uncoupling mitochondrial protein 1
Primary source: TAIR:AT3G54110
Locus tag: AT3G54110
See related: Araport:AT3G54110
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1; ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1; ATPUMP1; ATUCP1; plant uncoupling mitochondrial protein 1; UCP; UCP1; UNCOUPLING PROTEIN 1
Summary: Member of Uncoupling protein PUMP2 family. Encodes a mitochondrial uncoupling protein AtUCP1 involved in maintain the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. Disruption of UCP1 results in a photosynthetic phenotype. Specifically there is a restriction in photorespiration with a decrease in the rate of oxidation of photorespiratory glycine in the mitochondrion. This change leads to an associated reduced photosynthetic carbon assimilation rate.
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Genomic context

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See PUMP1 in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 9

Sequence:: Chromosome: 3; NC_003074.8 (20038670..20041210)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

High-throughput analysis reveals disturbances throughout the cell caused by Arabidopsis UCP1 and UCP3 double knockdown.
Title: High-throughput analysis reveals disturbances throughout the cell caused by Arabidopsis UCP1 and UCP3 double knockdown.
UCP1 and AOX1a contribute to regulation of carbon and nitrogen metabolism and yield in Arabidopsis under low nitrogen stress.
Title: UCP1 and AOX1a contribute to regulation of carbon and nitrogen metabolism and yield in Arabidopsis under low nitrogen stress.
Knockdown of Mitochondrial Uncoupling Proteins 1 and 2 (AtUCP1 and 2) in Arabidopsis thaliana Impacts Vegetative Development and Fertility.
Title: Knockdown of Mitochondrial Uncoupling Proteins 1 and 2 (AtUCP1 and 2) in Arabidopsis thaliana Impacts Vegetative Development and Fertility.
Similar to the expression and activity of alternative oxidase, the transcript level of UCP4, UCP5 and the UCP activity increased due to Pseudomonas syringae harpin protein treatment and the consequential oxidative burst.
Title: Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst.
AtUCP1 and AtUCP2 are the first reported mitochondrial carriers in Arabidopsis to transport aspartate and glutamate
Title: Uncoupling proteins 1 and 2 (UCP1 and UCP2) from Arabidopsis thaliana are mitochondrial transporters of aspartate, glutamate, and dicarboxylates.
The transcriptome of overexpressing plants revealed a broad induction of stress-responsive genes not strictly related to the mitochondrial antioxidant machinery, suggesting that overexpression of AtUCP1 imposes a strong stress response within the cell.
Title: Overexpression of mitochondrial uncoupling protein 1 (UCP1) induces a hypoxic response in Nicotiana tabacum leaves.
Foliar NO3 (-) assimilation was enhanced in both aox1a and ucp1 compared with the wild-type, suggesting that foliar NO3 (-) assimilation is probably driven by a decreased capacity of mAET and an increase in reductant within the cytosol.
Title: Disruption of the mitochondrial alternative oxidase (AOX) and uncoupling protein (UCP) alters rates of foliar nitrate and carbon assimilation in Arabidopsis thaliana.
Overexpression of UCP1 in the mitochondrial inner membrane induced increased uncoupling respiration, decreased reactive pxygen species accumulation under abiotic stresses, and diminished cellular ATP content.
Title: Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response.
Cys28 substitution reduced ATP inhibitory effect, while Tyr269Phe mutant exhibited 2.8-fold increase in sensitivity to ATP.
Title: Mutational analysis of Arabidopsis thaliana plant uncoupling mitochondrial protein.
The main physiological role of UCP1 in Arabidopsis leaves is related to maintaining the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. [AtUCP1]
Title: Mitochondrial uncoupling protein is required for efficient photosynthesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_115271.5

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables amino acid transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables oxidative phosphorylation uncoupler activity	IDA Inferred from Direct Assay more info	PubMed
enables oxidative phosphorylation uncoupler activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within amino acid transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within photorespiration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within photosynthesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	HDA more info	PubMed
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
colocalizes_with mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISM Inferred from Sequence Model more info
located_in plant-type vacuole	HDA more info	PubMed
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: plant uncoupling mitochondrial protein 1

NP_190979.1

plant uncoupling mitochondrial protein 1 (PUMP1); FUNCTIONS IN: oxidative phosphorylation uncoupler activity, binding; INVOLVED IN: transport, photosynthesis, photorespiration; LOCATED IN: mitochondrion, mitochondrial inner membrane, plasma membrane, chloroplast; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Mitochondrial substrate carrier (InterPro:IPR001993), Mitochondrial brown fat uncoupling protein (InterPro:IPR002030), Mitochondrial substrate/solute carrier (InterPro:IPR018108); BEST Arabidopsis thaliana protein match is: uncoupling protein 2 (TAIR:AT5G58970.1); Has 26567 Blast hits to 13376 proteins in 459 species: Archae - 0; Bacteria - 2; Metazoa - 11203; Fungi - 7876; Plants - 4909; Viruses - 3; Other Eukaryotes - 2574 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

20038670..20041210

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_115271.5 → NP_190979.1 plant uncoupling mitochondrial protein 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_190979.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O65623, O81845, Q56Z57

UniProtKB/TrEMBL

A0A178V8U1, A0A5S9XKV5

Conserved Domains (1) summary

pfam00153
Location:110 → 206

Mito_carr; Mitochondrial carrier protein