PIN4 Auxin efflux carrier family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: PIN4
Official Full Name: Auxin efflux carrier family protein
Primary source: TAIR:AT2G01420
Locus tag: AT2G01420
See related: Araport:AT2G01420
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS PIN-FORMED 4; ATPIN4; AUXIN TRANSPORTER SPLICE VARIANT B; F10A8.27; PIN-FORMED 4
Summary: Encodes a putative auxin efflux carrier that is localized in developing and mature root meristems. It is involved in the maintenance of embryonic auxin gradients. A role for AtPIN4 in generating a sink for auxin below the quiescent center of the root meristem that is essential for auxin distribution and patterning is proposed. In the root, PIN4 is detected around the quiescent center and cells surrounding it, and localizes basally in provascular cells. PIN4 expression is upregulated in brassinosteroid-insensitive mutant (PMID 16141452).
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Genomic context

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See PIN4 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 6

Sequence:: Chromosome: 2; NC_003071.7 (180033..183764, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PIN4 role in shoot branching.
Title: Cytokinin Targets Auxin Transport to Promote Shoot Branching.
ABA treatment reduced the expression levels of the PIN-FORMEDs (PIN) auxin efflux carriers, PIN1, PIN3, PIN4, and PIN7, to a greater extent in the root meristems of arf2-101 mutant than in the wild type. ARF2 positively mediates the transcripts of transcription factor PLETHORA 1 (PLT1) gene but negatively mediates PLT2 at protein level in root meristems.
Title: ARF2 coordinates with PLETHORAs and PINs to orchestrate ABA-mediated root meristem activity in Arabidopsis .
Functional interplay between protein kinase CK2 and salicylic acid sustains PIN4 transcriptional expression.
Title: Functional interplay between protein kinase CK2 and salicylic acid sustains PIN transcriptional expression and root development.
Data show that phototropic hypocotyl bending is strongly dependent on the activity of D6PKs and the PIN-FORMED (PIN) auxin efflux facilitators PIN3, PIN4, and PIN7.
Title: D6PK AGCVIII kinases are required for auxin transport and phototropic hypocotyl bending in Arabidopsis.
NOV is required for cell type-specific expression of PIN4 in the root.
Title: NO VEIN mediates auxin-dependent specification and patterning in the Arabidopsis embryo, shoot, and root.
PP2A and PINOID both partially colocalize with PINs and act antagonistically on the phosphorylation state of their central hydrophilic loop, hence mediating PIN apical-basal polar targeting.
Title: Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux.
Data suggest that auxin transport activity, in particular that of the PIN-FORMED1 (PIN1) and PIN4 proteins, is a major factor in the maintenance of auxin gradients.
Title: Maintenance of embryonic auxin distribution for apical-basal patterning by PIN-FORMED-dependent auxin transport in Arabidopsis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MASC_STS14466 (e-PCR)
- UniSTS:258716

NM_001335061.1

Supported by ESTs:

NM_126203.3

Supported by ESTs:

NM_179592.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables auxin efflux transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in auxin export across the plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within auxin polar transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pattern specification process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within root development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell periphery	ISS Inferred from Sequence or Structural Similarity more info
located_in mitochondrion	HDA more info	PubMed
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: Auxin efflux carrier family protein

NP_565261.1

PIN-FORMED 4 (PIN4); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity; INVOLVED IN: root development, pattern specification process, auxin polar transport; LOCATED IN: endomembrane system, integral to membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1811 Blast hits to 1602 proteins in 498 species: Archae - 31; Bacteria - 1046; Metazoa - 12; Fungi - 10; Plants - 485; Viruses - 0; Other Eukaryotes - 227 (source: NCBI BLink).

NP_849923.1

PIN-FORMED 4 (PIN4); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity; INVOLVED IN: root development, pattern specification process, auxin polar transport; LOCATED IN: endomembrane system, integral to membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1823 Blast hits to 1616 proteins in 502 species: Archae - 31; Bacteria - 1046; Metazoa - 18; Fungi - 10; Plants - 487; Viruses - 2; Other Eukaryotes - 229 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

180033..183764 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_126203.3 → NP_565261.1 Auxin efflux carrier family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_565261.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A5S9WWA4, A0A654ER02

Conserved Domains (1) summary

pfam03547
Location:451 → 607

Mem_trans; Membrane transport protein
NM_179592.2 → NP_849923.1 Auxin efflux carrier family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_849923.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8RWZ6, Q9M7Q6, Q9MAS3

UniProtKB/TrEMBL

A0A178VZS2, A0A5S9WWA4, A0A654ER02

Conserved Domains (1) summary

pfam03547
Location:455 → 611

Mem_trans; Membrane transport protein
NM_001335061.1 → NP_001323732.1 Auxin efflux carrier family protein [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A178W185, A0A1P8AY30

Conserved Domains (2) summary

pfam03547
Location:9 → 190

Mem_trans; Membrane transport protein

cl22894
Location:455 → 517

Mem_trans; Membrane transport protein