RPT2a regulatory particle AAA-ATPase 2A [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: RPT2a
Official Full Name: regulatory particle AAA-ATPase 2A
Primary source: TAIR:AT4G29040
Locus tag: AT4G29040
See related: Araport:AT4G29040
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: 26S PROTEASOME AAA-ATPASE SUBUNIT RPT2A; F19B15.70; F19B15_70; regulatory particle AAA-ATPase 2A
Summary: RPT2a encodes the 26S proteasome subunit. It is required for root meristem maintenance, and regulates gametogenesis. RPT2a is also shown to regulate gene silencing via DNA methylation.
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Genomic context

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See RPT2a in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 6

Sequence:: Chromosome: 4; NC_003075.7 (14312283..14314600)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RPT2a enhances transgene post-transcriptional gene silencing by promoting the accumulation of transgene-derived short interfering RNAs without affecting their biogenesis.
Title: Proteasome subunit RPT2a promotes PTGS through repressing RNA quality control in Arabidopsis.
The authors showed a novel mechanism that a SUMO ligase AtMMS21 regulates activity of the 26S proteasome in root development of Arabidopsis. The in vitro and in vivo data supported that AtMMS21 interacts with RPT2a, a subunit of the 26S proteasome.
Title: A SUMO ligase AtMMS21 regulates activity of the 26S proteasome in root development.
Both the transgene and endogenous RD29A promoter regions were hypermethylated at CG and non-CG contexts in the rpt2a mutant.
Title: Arabidopsis RPT2a, 19S proteasome subunit, regulates gene silencing via DNA methylation.
Loss of RPT2a destabilizes the 26S proteasome.
Title: The RPT2 subunit of the 26S proteasome directs complex assembly, histone dynamics, and gametophyte and sporophyte development in Arabidopsis.
RPT2a and RPT2b contribute differently to the proteasome activity required for root apical meristem growth and gametophyte development.
Title: Arabidopsis RPT2a encoding the 26S proteasome subunit is required for various aspects of root meristem maintenance, and regulates gametogenesis redundantly with its homolog, RPT2b.
RPT2a and RPT2b, the subunits of the 26S proteasome AAA-ATPase, are UNI-interacting proteins. uni-1D-induced morphological phenotypes during early vegetative stage were suppressed by loss of function of RPT2a, but not RPT2b.
Title: RPT2a, a 26S proteasome AAA-ATPase, is directly involved in Arabidopsis CC-NBS-LRR protein uni-1D-induced signaling pathways.
RPT2a and RPT5a are involved in Zn deficiency-tolerance, possibly through alleviation of oxidative stresses and/or processing of poly-ubiquitinated proteins.
Title: Arabidopsis thaliana 26S proteasome subunits RPT2a and RPT5a are crucial for zinc deficiency-tolerance.
Data suggest that RPT2a functions to negatively regulate endocycle progression following completion of the third endoreduplication step mediated by RHL1.
Title: Control of endoreduplication of trichome by RPT2a, a subunit of the 19S proteasome in Arabidopsis.
RPT2a has a specific role in the regulation of leaf organ size that involves control of endoreduplication and cell size, rather than alteration of leaf cell number.
Title: Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_119048.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IGI Inferred from Genetic Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within female gamete generation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within inflorescence development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lateral root formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within leaf morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within maintenance of root meristem identity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within male gamete generation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within meristem growth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meristem structural organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phyllome development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within proteasome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in proteasome core complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to misfolded protein	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within root cap development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within root development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within root morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within seedling development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within shoot system development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
NOT located_in cytosol	RCA inferred from Reviewed Computational Analysis more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info
located_in nucleus	TAS Traceable Author Statement more info	PubMed
located_in plasma membrane	HDA more info	PubMed
part_of proteasome complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome regulatory particle, base subcomplex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: regulatory particle AAA-ATPase 2A

NP_194633.1

regulatory particle AAA-ATPase 2A (RPT2a); FUNCTIONS IN: ATPase activity; INVOLVED IN: in 17 processes; LOCATED IN: proteasome regulatory particle, base subcomplex, proteasome complex, nucleus, membrane; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: AAA-type ATPase family protein (TAIR:AT2G20140.1); Has 31112 Blast hits to 28760 proteins in 3112 species: Archae - 1431; Bacteria - 10308; Metazoa - 4877; Fungi - 3744; Plants - 3214; Viruses - 41; Other Eukaryotes - 7497 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

14312283..14314600

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_119048.4 → NP_194633.1 regulatory particle AAA-ATPase 2A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_194633.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9SEI5, Q9SZD4

UniProtKB/TrEMBL

A0A178V3V3, A0A5S9WZI7, A0A5S9XWV4

Conserved Domains (1) summary

PTZ00361
Location:7 → 443

PTZ00361; 26 proteosome regulatory subunit 4-like protein; Provisional