AGO4 Argonaute family protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: AGO4
Official Full Name: Argonaute family protein
Primary source: TAIR:AT2G27040
Locus tag: AT2G27040
See related: Araport:AT2G27040
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARGONAUTE 4; OCP11; OVEREXPRESSOR OF CATIONIC PEROXIDASE 11; T20P8.9; T20P8_9
Summary: AGO4 is a member of a class of PAZ/PIWI domain containing proteins involved in siRNA mediated gene silencing.Loss of function mutations have reduced site specific CpNpG and CpHpH methylation and increased susceptibility to bacterial pathogens.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See AGO4 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 23

Sequence:: Chromosome: 2; NC_003071.7 (11536502..11542391, complement)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation.
Title: The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation.
The canonical RdDM pathway mediates the control of seed germination timing under salinity.
Title: The canonical RdDM pathway mediates the control of seed germination timing under salinity.
We show that canonical miRNAs predominantly associate with a high molecular weight (HMW) RISC which co-migrates with AGO1. By providing HTS data we show that the 24-nt siRNAs are mainly associated with a low molecular weight (LMW) RISC co-migrating with AGO4
Title: AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loading.
DDB2 forms a complex with ROS1 and AGO4 and that they act at the ROS1 locus to modulate levels of DNA methylation and therefore ROS1 expression. DDB2 acts as a critical regulator of ROS1-mediated active DNA demethylation.
Title: Dual control of ROS1-mediated active DNA demethylation by DNA damage-binding protein 2 (DDB2).
These results indicate an important role for AGO4 in targeting plant RNA viruses as well as demonstrating novel mechanisms of regulation of and by AGO4, independent of its canonical role in regulating gene expression by RNA-dependent DNA methylation (RdDM).
Title: Antiviral Defense Involves AGO4 in an Arabidopsis-Potexvirus Interaction.
Data suggest that AGO4-dependent siRNAs are secondary siRNAs dependent on the prior activity of the RdDM pathway at certain loci.
Title: AGO4 is specifically required for heterochromatic siRNA accumulation at Pol V-dependent loci in Arabidopsis thaliana.
demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine-tryptophan/tryptophan-glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L.
Title: Evidence for ARGONAUTE4-DNA interactions in RNA-directed DNA methylation in plants.
Findings reported here highlight an involvement of AGO4 during meiosis by ensuring accurate chromosome segregation at anaphase I.
Title: Accurate Chromosome Segregation at First Meiotic Division Requires AGO4, a Protein Involved in RNA-Dependent DNA Methylation in Arabidopsis thaliana.
Members of the AGO4 clade cooperatively participate in preventing the abnormal specification of multiple premeiotic gametophytic precursors during early ovule development.
Title: A Multigenic Network of ARGONAUTE4 Clade Members Controls Early Megaspore Formation in Arabidopsis.
Our results indicate that AtMBD6 is involved in RNA-mediated gene silencing and it binds to RNA binding proteins like AtRPS2C, AtAGO4 and AtNTF2. AtMBD6 also interacts with histone deacetylase AtHDA6 that might have a role in chromatin condensation at the targets of RdDM
Title: AtMBD6, a methyl CpG binding domain protein, maintains gene silencing in Arabidopsis by interacting with RNA binding proteins.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

MASC_STS16977 (e-PCR)
- UniSTS:258917

NM_001202684.1

Supported by ESTs:

NM_128262.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to virus	IDA Inferred from Direct Assay more info	PubMed
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulatory ncRNA-mediated heterochromatin formation	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within siRNA processing	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within siRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in siRNA-mediated long-distance post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Cajal body	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info
NOT located_in cytosol	RCA inferred from Reviewed Computational Analysis more info	PubMed
located_in euchromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: Argonaute family protein

NP_001189613.1

ARGONAUTE 4 (AGO4); CONTAINS InterPro DOMAIN/s: Domain of unknown function DUF1785 (InterPro:IPR014811), Stem cell self-renewal protein Piwi (InterPro:IPR003165), Argonaute/Dicer protein, PAZ (InterPro:IPR003100), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337); BEST Arabidopsis thaliana protein match is: Argonaute family protein (TAIR:AT5G21150.1).

NP_565633.1

ARGONAUTE 4 (AGO4); FUNCTIONS IN: siRNA binding; INVOLVED IN: in 6 processes; LOCATED IN: nuclear euchromatin, nucleolus, Cajal body; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Domain of unknown function DUF1785 (InterPro:IPR014811), Stem cell self-renewal protein Piwi (InterPro:IPR003165), Argonaute/Dicer protein, PAZ (InterPro:IPR003100), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337); BEST Arabidopsis thaliana protein match is: Argonaute family protein (TAIR:AT5G21150.1); Has 1929 Blast hits to 1875 proteins in 205 species: Archae - 0; Bacteria - 0; Metazoa - 1009; Fungi - 290; Plants - 478; Viruses - 0; Other Eukaryotes - 152 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

11536502..11542391 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_128262.4 → NP_565633.1 Argonaute family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_565633.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q93VG2, Q9ZVD5

UniProtKB/TrEMBL

A0A178VSD8

Conserved Domains (1) summary

PLN03202
Location:45 → 924

PLN03202; protein argonaute; Provisional
NM_001202684.1 → NP_001189613.1 Argonaute family protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001189613.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q93VG2, Q9ZVD5

UniProtKB/TrEMBL

A0A178VSD8

Conserved Domains (1) summary

PLN03202
Location:45 → 924

PLN03202; protein argonaute; Provisional