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    MAP65-1 microtubule-associated proteins 65-1 [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 835616, updated on 18-Sep-2024

    Summary

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    Official Symbol
    MAP65-1
    Official Full Name
    microtubule-associated proteins 65-1
    Primary source
    TAIR:AT5G55230
    Locus tag
    AT5G55230
    See related
    Araport:AT5G55230
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ATMAP65-1; MCO15.18; MCO15_18; microtubule-associated proteins 65-1
    Summary
    Binds and bundles microtubules. Plays a role in stabilizing anti-parallel microtubules in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. The timing with which the MAP65-1 was targeted to the spindle appears to be regulated by a phosphorylation sensitive switch. Enhances microtubule polymerization, promotes nucleation and stabilizes microtubules against cold treatment and dilution.
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    Genomic context

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    See MAP65-1 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    10
    Sequence:
    Chromosome: 5; NC_003076.8 (22401927..22405568)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene Co-chaperone GrpE family protein Neighboring gene uncharacterized protein Neighboring gene trigger factor type chaperone family protein Neighboring gene PEROXYGENASE 2 Neighboring gene IAA carboxylmethyltransferase 1

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. MAP65-1 is required for the depolymerization and reorganization of cortical microtubules in the response to salt stress in Arabidopsis. Zhou S, et al. Plant Sci, 2017 Nov. PMID 28969791
    2. The Arabidopsis microtubule-associated protein AtMAP65-1: molecular analysis of its microtubule bundling activity. Smertenko AP, et al. Plant Cell, 2004 Aug. PMID 15273298, Free PMC Article
    3. Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression. Boruc J, et al. Plant Physiol, 2017 Jan. PMID 27879390, Free PMC Article
    4. In vivo dynamics and differential microtubule-binding activities of MAP65 proteins. Van Damme D, et al. Plant Physiol, 2004 Dec. PMID 15557096, Free PMC Article
    5. Modulated targeting of GFP-AtMAP65-1 to central spindle microtubules during division. Mao G, et al. Plant J, 2005 Aug. PMID 16098102

    See all (24) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Work reveals that cross-linking of microtubules by MAP65-1 confers resistance to severing by inhibiting the binding of KTN1 and identifies the structural features of MAP65-1 that are important for this activity.
      Title: Microtubule bundling by MAP65-1 protects against severing by inhibiting the binding of katanin.
    2. MAP65-1 plays a critical role in the response to salt stress and is required for regulating the rapid depolymerization and reorganization of cortical microtubules (MTs).
      Title: MAP65-1 is required for the depolymerization and reorganization of cortical microtubules in the response to salt stress in Arabidopsis.
    3. MAP65-1 is subject to multiple phosphorylation events by Aurora kinases during consecutive stages of the cell cycle.
      Title: Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression.
    4. Data indicate that microtubules (MTs) bundle flexibility is differently regulated, depending on MAP65 cross-linker.
      Title: MAP65/Ase1 promote microtubule flexibility.
    5. PLDalpha1-derived phosphatidic acid (PA) binds to MAP65-1, thus mediating microtubule stabilization and salt tolerance. It reveals a functional connection between membrane lipids and the cytoskeleton in environmental stress signaling.
      Title: Phosphatidic acid regulates microtubule organization by interacting with MAP65-1 in response to salt stress in Arabidopsis.
    6. MAP65-1 and MAP65-2 play a critical role in the microtubule-dependent mechanism for specifying axial cell growth in the expanding hypocotyl, independent of any mechanical role in microtubule array organization.
      Title: Microtubule-associated proteins MAP65-1 and MAP65-2 positively regulate axial cell growth in etiolated Arabidopsis hypocotyls.
    7. MAP65-1 and MAP65-2 play a critical role in root growth by promoting cell proliferation and axial extension.
      Title: MAP65-1 and MAP65-2 promote cell proliferation and axial growth in Arabidopsis roots.
    8. Results also show that MAP65-1 and MAP65-3 may share redundant functions in MT stabilization.
      Title: Arabidopsis microtubule-associated protein MAP65-3 cross-links antiparallel microtubules toward their plus ends in the phragmoplast via its distinct C-terminal microtubule binding domain.
    9. the rod domain of MAP65-1 acts both as a spacer and as a structural element that specifies the microtubule encounter angles that are conducive for bundling
      Title: Single-molecule analysis of the microtubule cross-linking protein MAP65-1 reveals a molecular mechanism for contact-angle-dependent microtubule bundling.
    10. MAP65-1 binds cortical microtubules.
      Title: [Localization of microtubule-associated protein AtMAP65-1 in guard cells of arabidopsis].
    Submit: New GeneRIF Correction See all GeneRIFs (16)

    General gene information

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    NM_001203613.1
    NM_001345120.1
    NM_124905.5

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables microtubule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein dimerization activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cell growth IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in cortical microtubule organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cytokinesis by cell plate formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within microtubule bundle formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within microtubule nucleation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within microtubule polymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of microtubule polymerization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within post-embryonic root development IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cortical microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cortical microtubule, transverse to long axis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm HDA PubMed 
    located_in microtubule IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitotic spindle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  
    located_in phragmoplast HDA PubMed 
    located_in phragmoplast IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plastid HDA PubMed 
    located_in preprophase band IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in spindle HDA PubMed 
    located_in spindle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    microtubule-associated proteins 65-1
    NP_001190542.1
    • microtubule-associated proteins 65-1 (MAP65-1); FUNCTIONS IN: microtubule binding; INVOLVED IN: in 6 processes; LOCATED IN: in 7 components; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Microtubule-associated protein, MAP65/ASE1-type (InterPro:IPR007145); BEST Arabidopsis thaliana protein match is: microtubule-associated protein 65-2 (TAIR:AT4G26760.1).
    NP_200334.1
    • microtubule-associated proteins 65-1 (MAP65-1); FUNCTIONS IN: microtubule binding; INVOLVED IN: in 6 processes; LOCATED IN: in 7 components; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Microtubule-associated protein, MAP65/ASE1-type (InterPro:IPR007145); BEST Arabidopsis thaliana protein match is: microtubule-associated protein 65-2 (TAIR:AT4G26760.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      22401927..22405568
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_124905.5 → NP_200334.1  microtubule-associated proteins 65-1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_200334.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9FLP0
      UniProtKB/TrEMBL
      A0A178UKF6, A0A5S9YEW0
      Conserved Domains (1) summary
      pfam03999
      Location:56 → 550
      MAP65_ASE1; Microtubule associated protein (MAP65/ASE1 family)

    2. NM_001345120.1 → NP_001332196.1  microtubule-associated proteins 65-1 [Arabidopsis thaliana]

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9FLP0
      UniProtKB/TrEMBL
      A0A178UKF6, A0A5S9YEW0
      Conserved Domains (1) summary
      pfam03999
      Location:56 → 550
      MAP65_ASE1; Microtubule associated protein (MAP65/ASE1 family)

    3. NM_001203613.1 → NP_001190542.1  microtubule-associated proteins 65-1 [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_001190542.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      A0A5S9YEW0, F4K3E4
      Conserved Domains (1) summary
      pfam03999
      Location:56 → 616
      MAP65_ASE1; Microtubule associated protein (MAP65/ASE1 family)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9FLP0.1

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