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    DEG8 Trypsin family protein with PDZ domain-containing protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 833979, updated on 18-Sep-2024

    Summary

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    Official Symbol
    DEG8
    Official Full Name
    Trypsin family protein with PDZ domain-containing protein
    Primary source
    TAIR:AT5G39830
    Locus tag
    AT5G39830
    See related
    Araport:AT5G39830
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    DEG PROTEASE 8; DEGP8; degradation of periplasmic proteins 8; K13H13.1; K13H13_1
    Summary
    Encodes DEG8. Forms a hexamer with DEG5 in the thylakoid lumen. Involved in the cleavage of photodamaged D2 protein of photosystem II (PSII). Recombinant DEG8 is proteolytically active toward both a model substrate (beta-casein) and photodamaged D1 protein of photosystem II.
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    Genomic context

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    See DEG8 in Genome Data Viewer
    Location:
    chromosome: 5
    Exon count:
    13
    Sequence:
    Chromosome: 5; NC_003076.8 (15942764..15945897)

    Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene AGAMOUS-like 98 Neighboring gene NAC domain containing protein 94 Neighboring gene ATP-dependent RNA helicase Neighboring gene Ribosomal protein S4

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003076.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana Deg8. Shan X, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2013 Jan 1. PMID 23295491, Free PMC Article
    2. The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases. Sun W, et al. Acta Crystallogr D Biol Crystallogr, 2013 May. PMID 23633592, Free PMC Article
    3. Differential Roles of the Thylakoid Lumenal Deg Protease Homologs in Chloroplast Proteostasis. Butenko Y, et al. Plant Physiol, 2018 Nov. PMID 30237207, Free PMC Article
    4. Formation of DEG5 and DEG8 complexes and their involvement in the degradation of photodamaged photosystem II reaction center D1 protein in Arabidopsis. Sun X, et al. Plant Cell, 2007 Apr. PMID 17449806, Free PMC Article
    5. Cooperative D1 degradation in the photosystem II repair mediated by chloroplastic proteases in Arabidopsis. Kato Y, et al. Plant Physiol, 2012 Aug. PMID 22698923, Free PMC Article

    See all (11) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. This work suggests that differences in protein abundance and proteolytic activity underlie the differential importance of Deg1 and Deg5-Deg8 protease complexes observed in vivo.
      Title: Differential Roles of the Thylakoid Lumenal Deg Protease Homologs in Chloroplast Proteostasis.
    2. crystal structure reveals that Deg8 protein contains one PDZ domain
      Title: The structures of Arabidopsis Deg5 and Deg8 reveal new insights into HtrA proteases.
    3. X-ray crystallographic analysis of Deg8 protein structure
      Title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana Deg8.
    4. These data demonstrate in vivo cooperative degradation of D1, in which Deg cleavage assists FtsH processive degradation under photoinhibitory conditions.
      Title: Cooperative D1 degradation in the photosystem II repair mediated by chloroplastic proteases in Arabidopsis.
    5. DEG5 and DEG8 have a synergistic function in the primary cleavage of the CD loop of the PSII reaction center protein D1. [DEG8]
      Title: Formation of DEG5 and DEG8 complexes and their involvement in the degradation of photodamaged photosystem II reaction center D1 protein in Arabidopsis.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    NM_123346.5
    NM_203134.2

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables peptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables serine-type endopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within photosystem II repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in chloroplast HDA PubMed 
    located_in chloroplast thylakoid HDA PubMed 
    located_in chloroplast thylakoid lumen HDA PubMed 
    located_in chloroplast thylakoid lumen IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  
    located_in plastid HDA PubMed 
    located_in thylakoid HDA PubMed 
    located_in thylakoid lumen HDA PubMed 

    General protein information

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    Preferred Names
    Trypsin family protein with PDZ domain-containing protein
    NP_568575.1
    • DEG8; FUNCTIONS IN: serine-type peptidase activity, peptidase activity; INVOLVED IN: photosystem II repair, proteolysis; LOCATED IN: thylakoid, thylakoid lumen, chloroplast thylakoid lumen, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Serine/cysteine peptidase, trypsin-like (InterPro:IPR009003), Peptidase S1C, HrtA/DegP2/Q/S (InterPro:IPR001940), Peptidase S1/S6, chymotrypsin/Hap (InterPro:IPR001254), PDZ/DHR/GLGF (InterPro:IPR001478); BEST Arabidopsis thaliana protein match is: DegP protease 1 (TAIR:AT3G27925.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    NP_974863.1
    • DEG8; FUNCTIONS IN: serine-type peptidase activity, peptidase activity; INVOLVED IN: photosystem II repair, proteolysis; LOCATED IN: thylakoid, chloroplast thylakoid lumen, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Serine/cysteine peptidase, trypsin-like (InterPro:IPR009003), Peptidase S1C, HrtA/DegP2/Q/S (InterPro:IPR001940), Peptidase S1/S6, chymotrypsin/Hap (InterPro:IPR001254), PDZ/DHR/GLGF (InterPro:IPR001478); BEST Arabidopsis thaliana protein match is: DegP protease 1 (TAIR:AT3G27925.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003076.8 Reference assembly

      Range
      15942764..15945897
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_123346.5 → NP_568575.1  Trypsin family protein with PDZ domain-containing protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_568575.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9LU10
      UniProtKB/TrEMBL
      A0A178ULP8, A0A5S9YAI1
      Conserved Domains (1) summary
      TIGR02037
      Location:119 → 445
      degP_htrA_DO; periplasmic serine protease, Do/DeqQ family

    2. NM_203134.2 → NP_974863.1  Trypsin family protein with PDZ domain-containing protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_974863.1

      Status: REVIEWED

      UniProtKB/TrEMBL
      F4KFV6
      Conserved Domains (2) summary
      cd00987
      Location:332 → 427
      PDZ_serine_protease; PDZ domain of trypsin-like serine proteases, such as DegP/HtrA, which are oligomeric proteins involved in heat-shock response, chaperone function, and apoptosis. May be responsible for substrate recognition and/or binding, as most PDZ domains bind ...
      pfam13365
      Location:155 → 306
      Trypsin_2; Trypsin-like peptidase domain
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9LU10.1

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