Wnk Wnk kinase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Wnkprovided by FlyBase
Official Full Name: Wnk kinaseprovided by FlyBase
Primary source: FLYBASE:FBgn0037098
Locus tag: Dmel_CG7177
See related: AllianceGenome:FB:FBgn0037098
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: cg7177; CG7177; Dmel\CG7177; DmWNK; dWnk; dWNK; DWNK; wnk; WNK
Summary: Enables molecular condensate scaffold activity and protein kinase activity. Involved in several processes, including cellular hyperosmotic response; positive regulation of canonical Wnt signaling pathway; and positive regulation of potassium ion import across plasma membrane. Predicted to be active in cytoplasm. Used to study pseudohypoaldosteronism. Human ortholog(s) of this gene implicated in Prieto syndrome; hereditary sensory and autonomic neuropathy type 2A; hereditary sensory neuropathy; hypertension; and pseudohypoaldosteronism. Orthologous to several human genes including WNK1 (WNK lysine deficient protein kinase 1); WNK2 (WNK lysine deficient protein kinase 2); and WNK3 (WNK lysine deficient protein kinase 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 78D5-78D6; 3-47 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (21543850..21554928, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (21536950..21548028, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Unanticipated domain requirements for Drosophila Wnk kinase in vivo.
Title: Unanticipated domain requirements for Drosophila Wnk kinase in vivo.
SIK3 and Wnk converge on Fray to regulate glial K+ buffering and seizure susceptibility.
Title: SIK3 and Wnk converge on Fray to regulate glial K+ buffering and seizure susceptibility.
Axon morphogenesis and maintenance require an evolutionary conserved safeguard function of Wnk kinases antagonizing Sarm and Axed.
Title: Axon morphogenesis and maintenance require an evolutionary conserved safeguard function of Wnk kinases antagonizing Sarm and Axed.
WNKs are potassium-sensitive kinases.
Title: WNKs are potassium-sensitive kinases.
The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system.
Title: The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system.
The Drosophila WNK-SPAK/OSR1-NKCC cascade is an essential molecular pathway for osmoregulation.
Title: Hypotonicity stimulates potassium flux through the WNK-SPAK/OSR1 kinase cascade and the Ncc69 sodium-potassium-2-chloride cotransporter in the Drosophila renal tubule.
Drosophila WNK mutant caused defects in axon guidance during embryogenesis.
Title: WNK signaling is involved in neural development via Lhx8/Awh expression.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables molecular condensate scaffold activity	IDA Inferred from Direct Assay more info	PubMed
enables potassium channel inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell volume homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular hyperosmotic response	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in membraneless organelle assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in monoatomic ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of sodium ion transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of canonical Wnt signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of potassium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of potassium ion import across plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in wing disc development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: Wnk kinase

Names: CG7177-PL; CG7177-PM; CG7177-PN; CG7177-PO; WNK homolog; WNK kinase; Wnk-PL; Wnk-PM; Wnk-PN; Wnk-PO; with No lysine kinase; with-no-lysine kinase

NP_001262171.2

EC 2.7.11.1

non-AUG (AUU) translation initiation

NP_001262172.3

EC 2.7.11.1

non-AUG (AUU) translation initiation

NP_001262173.2

EC 2.7.11.1

NP_001262174.3

EC 2.7.11.1

non-AUG (AUU) translation initiation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

21543850..21554928 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001275242.2 → NP_001262171.2 Wnk kinase, isoform M [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

M9PD81, M9PG54, M9PGC5, M9PIH3, Q8MTA4

UniProtKB/TrEMBL

A0A075M5N0

Conserved Domains (3) summary

smart00220
Location:471 → 729

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd13983
Location:469 → 729

STKc_WNK; Catalytic domain of the Serine/Threonine kinase, With No Lysine (WNK) kinase

pfam12202
Location:754 → 791

OSR1_C; Oxidative-stress-responsive kinase 1 C terminal
NM_001275243.3 → NP_001262172.3 Wnk kinase, isoform N [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A075M5N0

Conserved Domains (3) summary

smart00220
Location:471 → 729

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd13983
Location:469 → 729

STKc_WNK; Catalytic domain of the Serine/Threonine kinase, With No Lysine (WNK) kinase

pfam12202
Location:754 → 791

OSR1_C; Oxidative-stress-responsive kinase 1 C terminal
NM_001275245.3 → NP_001262174.3 Wnk kinase, isoform L [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A075M5N0

Conserved Domains (3) summary

smart00220
Location:471 → 729

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd13983
Location:469 → 729

STKc_WNK; Catalytic domain of the Serine/Threonine kinase, With No Lysine (WNK) kinase

pfam12202
Location:754 → 791

OSR1_C; Oxidative-stress-responsive kinase 1 C terminal
NM_001275244.2 → NP_001262173.2 Wnk kinase, isoform O [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A075M5N0

Conserved Domains (3) summary

smart00220
Location:273 → 531

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd13983
Location:271 → 531

STKc_WNK; Catalytic domain of the Serine/Threonine kinase, With No Lysine (WNK) kinase

pfam12202
Location:556 → 593

OSR1_C; Oxidative-stress-responsive kinase 1 C terminal